Protein Dephosphorylation Methods
Determining phosphospecificity of phosphospecific antibodies is serious business to PhosphoSolutions. This step-by-step method is broken down into 2 major sections on how to dephosphorylate proteins the right way. Within each section there is a detailed list of steps, buffers, specific materials, and calculation examples to help researchers through this comprehensive process. Additionally, helpful technical tips are offered throughout the protocol to provide insight about various factors that should be considered when performing protein dephosphorylation. Our goal is to provide essential techniques and tools to help researchers obtain publishable and reproducible results.