Nonionic detergents such as NP-40 or triton fail to solubilize many cellular proteins involved in cell signaling. This problem is particularly acute in brain where synaptic junctions are known to be insoluble in nonionic detergents. 1% SDS completely solubilizes membrane and other hard to solubilize proteins including synaptic junction proteins. Suitable for both organs and tissue culture cells.
- 15 mL
- 10% (w/v) SDS, 100 mM TRIS, 10 mM EDTA, pH 8.0. Dilute to 1X with dH2O before use.