10X Western Blot Lysis Buffer

Catalog #: 100-LYS Category: Datasheet:

$75.00

Great for fully solubilizing proteins in preparation for Western blotting!

Nonionic detergents such as NP-40 or triton fail to solubilize many cellular proteins involved in cell signaling. This problem is particularly acute in brain where synaptic junctions are known to be insoluble in nonionic detergents. 1% SDS completely solubilizes membrane and other hard to solubilize proteins including synaptic junction proteins. Suitable for both organs and tissue culture cells.

Size:
15 mL
Formulation:
10% (w/v) SDS, 100 mM TRIS, 10 mM EDTA, pH 8.0. Dilute to 1X with dH2O before use.

Storage

Storage at room temperature is recommended for both 10X and 1X solutions to prevent SDS from precipitating out of solution.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Inconclusive
  • 1 – Didn't work
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