Protocols for the Discerning Scientist

Essential techniques to help you obtain publishable and reproducible results.

Western Blot Protocol

Broken down into 5 sections: sample prep, SDS PAGE, protein transfer, immunolabeling, and imaging, our WB protocol explains and highlights various factors that should be considered in your experiment.

Lysate Preparation Protocol

Learn how to properly lyse organs and tissue culture cells when focusing on preserving protein phosphorylation, including detailed differences in lysing adherent and suspension tissue culture cells.

Protein Dephosphorylation Methods

This step-by-step method is broken down into 2 major sections on how to dephosphorylate proteins the right way. Within each section there is a detailed list of steps, buffers, specific materials, and calculation examples.