Western Blot Protocol
Broken down into 5 sections: sample prep, SDS PAGE, protein transfer, immunolabeling, and imaging, our WB protocol explains and highlights various factors that should be considered in your experiment.
Lysate Preparation Protocol
Learn how to properly lyse organs and tissue culture cells when focusing on preserving protein phosphorylation, including detailed differences in lysing adherent and suspension tissue culture cells.
Protein Dephosphorylation Methods
This step-by-step method is broken down into 2 major sections on how to dephosphorylate proteins the right way. Within each section there is a detailed list of steps, buffers, specific materials, and calculation examples.