Lysate Preparation Protocol
PhosphoSolutions’ step-by-step protocol shows how to properly lyse organs and tissue culture cells when focusing on preserving protein phosphorylation, including detailed differences in lysing adherent and suspension tissue culture cells. Within each section there is a detailed list of steps, buffers, and specific materials to help researchers through this process. Additionally, helpful technical tips are offered throughout the protocol to provide insight about various factors that should be considered when lysing organs and tissue culture cells. Our goal is to provide essential techniques or tools to help researchers obtain publishable and reproducible results.