10X Lysis Buffer

Name: 10X Lysis Buffer
Brand: PhosphoSolutions
SKU: 100-LYS
Price: 105.0 USD
Availability: InStock

from PhosphoSolutions Datasheet

Optimized to fully solubilize proteins in preparation for Western blotting. Nonionic detergents such as NP-40 or triton fail to solubilize many cellular proteins involved in cell signaling. This problem is particularly acute in brain where synaptic junctions are known to be insoluble in nonionic detergents. 1% SDS completely solubilizes membrane and other hard to solubilize proteins including synaptic junction proteins. Suitable for both organs and tissue culture cells.

Product Type

Lysis Buffer

Format

SDS Lysis Buffer

10X Lysis Buffer

Volume: 15 mL

Variant

Price:

$105

Quantity:

Format

SDS Lysis Buffer

Storage

Storage at room temperature is recommended for both 10X and 1X solutions to prevent SDS from precipitating out of solution.

Physical State

Liquid

Buffer

10% (w/v) SDS, 100 mM TRIS, 10 mM EDTA, pH 8.0. Dilute to 1X with dH2O before use.

Usage Statement

This is a research laboratory reagent and is not for use as diagnostic or therapeutic reagents in humans.

Shipping

Ambient

PMID	Publication
35879879	Jablon, K.L., et al. 2022. Isolation and Short-Term Culturing of Primary Lymphatic Endothelial Cells from Collecting Lymphatics: A Techniques Study. Microcirculation, e12778.
35331847	Lloyd, J.T., et al. 2022. Dopamine Dysregulation and Altered Responses to Drugs Affecting Dopaminergic Transmission in a New Dopamine Transporter Knockout (DAT-KO) Rat Model. Neuroscience, 491, pp43-64.
35276206	Cerqueira, S.R., et al. 2022. BET protein inhibition promotes non-myeloid cell mediated neuroprotection after rodent spinal cord contusion. Experimental Neurology, 352, 114035.