Optimized to fully solubilize proteins in preparation for Western blotting. Nonionic detergents such as NP-40 or triton fail to solubilize many cellular proteins involved in cell signaling. This problem is particularly acute in brain where synaptic junctions are known to be insoluble in nonionic detergents. 1% SDS completely solubilizes membrane and other hard to solubilize proteins including synaptic junction proteins. Suitable for both organs and tissue culture cells.

Lysis Buffer
SDS Lysis Buffer

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10X Lysis Buffer
SKU: 100-LYS

Volume: 15 mL
Sale price$105

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Product Specific References

PMID Publication
35879879Jablon, K.L., et al. 2022. Isolation and Short-Term Culturing of Primary Lymphatic Endothelial Cells from Collecting Lymphatics: A Techniques Study. Microcirculation, e12778.
35331847 Lloyd, J.T., et al. 2022. Dopamine Dysregulation and Altered Responses to Drugs Affecting Dopaminergic Transmission in a New Dopamine Transporter Knockout (DAT-KO) Rat Model. Neuroscience, 491, pp43-64.
35276206 Cerqueira, S.R., et al. 2022. BET protein inhibition promotes non-myeloid cell mediated neuroprotection after rodent spinal cord contusion. Experimental Neurology, 352, 114035.

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