Optimized to fully solubilize proteins in preparation for Western blotting. Nonionic detergents such as NP-40 or triton fail to solubilize many cellular proteins involved in cell signaling. This problem is particularly acute in brain where synaptic junctions are known to be insoluble in nonionic detergents. 1% SDS completely solubilizes membrane and other hard to solubilize proteins including synaptic junction proteins. Suitable for both organs and tissue culture cells.
Lysis Buffer
SDS Lysis Buffer
Product Specific References
PMID | Publication |
37245409 | Heenatigala Palliyage, G, et al. 2023. Chemotherapy-induced PDL-1 Expression in Cancer-associated Fibroblasts Promotes Chemoresistance in NSCLC. Lung Cancer, 107258. |
37141550 | Brett, JO, et al. 2023. A Gene Panel Associated With Abemaciclib Utility in ESR1-Mutated Breast Cancer After Prior Cyclin-Dependent Kinase 4/6-Inhibitor Progression. JCO Precision Oncology, e2200532. |
36988414 | Endo, Y., et al. 2023. VEGFA Promotes Skeletal Muscle Regeneration in Aging. Advanced Biology, e2200320. |
35879879 | Jablon, K.L., et al. 2022. Isolation and Short-Term Culturing of Primary Lymphatic Endothelial Cells from Collecting Lymphatics: A Techniques Study. Microcirculation, e12778. |
35331847 | Lloyd, J.T., et al. 2022. Dopamine Dysregulation and Altered Responses to Drugs Affecting Dopaminergic Transmission in a New Dopamine Transporter Knockout (DAT-KO) Rat Model. Neuroscience, 491, pp43-64. |
35276206 | Cerqueira, S.R., et al. 2022. BET protein inhibition promotes non-myeloid cell mediated neuroprotection after rodent spinal cord contusion. Experimental Neurology, 352, 114035. |
Product Specific Protocols
- Western Blot Protocol: Download