Optimized to fully solubilize proteins in preparation for Western blotting. Nonionic detergents such as NP-40 or triton fail to solubilize many cellular proteins involved in cell signaling. This problem is particularly acute in brain where synaptic junctions are known to be insoluble in nonionic detergents. 1% SDS completely solubilizes membrane and other hard to solubilize proteins including synaptic junction proteins. Suitable for both organs and tissue culture cells.
Lysis Buffer
SDS Lysis Buffer
Product Specific References
PMID | Publication |
35879879 | Jablon, K.L., et al. 2022. Isolation and Short-Term Culturing of Primary Lymphatic Endothelial Cells from Collecting Lymphatics: A Techniques Study. Microcirculation, e12778. |
35331847 | Lloyd, J.T., et al. 2022. Dopamine Dysregulation and Altered Responses to Drugs Affecting Dopaminergic Transmission in a New Dopamine Transporter Knockout (DAT-KO) Rat Model. Neuroscience, 491, pp43-64. |
35276206 | Cerqueira, S.R., et al. 2022. BET protein inhibition promotes non-myeloid cell mediated neuroprotection after rodent spinal cord contusion. Experimental Neurology, 352, 114035. |