Optimized to fully solubilize proteins in preparation for Western blotting. Nonionic detergents such as NP-40 or triton fail to solubilize many cellular proteins involved in cell signaling. This problem is particularly acute in brain where synaptic junctions are known to be insoluble in nonionic detergents. 1% SDS completely solubilizes membrane and other hard to solubilize proteins including synaptic junction proteins. Suitable for both organs and tissue culture cells.
Product Specific References
|35331847||Lloyd, J.T., et al. 2022. Dopamine Dysregulation and Altered Responses to Drugs Affecting Dopaminergic Transmission in a New Dopamine Transporter Knockout (DAT-KO) Rat Model. Neuroscience, 491, pp43-64.|
|35276206||Cerqueira, S.R., et al. 2022. BET protein inhibition promotes non-myeloid cell mediated neuroprotection after rodent spinal cord contusion. Experimental Neurology, 352, 114035.|