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10X Lysis Buffer
PhosphoSolutions 10X Lysis Buffer
$115
Immunostaining of salamander retina showing labeling of 14-3-3 protein when phosphorylated at Ser58(cat. p1433-58, green 1:500) in Müller glial cells. The blue is staining DNA. Photo courtesy of Alex Vila, University of Texas at Houston.Western blot of rat brainstem lysate showing specific immunolabeling of the ~29 kDa 14-3-3 protein phosphorylated at Ser58 (-). The immunolabeling is blocked by the phosphopeptide used as the antigen (+) but not by the corresponding non-phosphopeptide (not shown).
PhosphoSolutions Anti-14-3-3 (Ser58) Antibody
From $155
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Western blot of rat hippocampal lysate showing specific immunolabeling of the ~29 kDa 14-3-3 protein.
PhosphoSolutions Anti-14-3-3 Protein Antibody
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Western blot of rat cortical lysate showing specific immunolabeling of the ~80 kDa doublet of 5-LO phosphorylated at Ser523 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).
PhosphoSolutions Anti-5-Lipoxygenase (Ser523) Antibody
From $155
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Western blot of human Jurkat cell lysate. The blot was probed with mouse monoclonal anti-A-Raf (N-terminal region) antibody at 1:500 (lane 1) and 1:2000 (lane 2).
PhosphoSolutions Anti-A-Raf (N-terminal region) Antibody
$275
PhosphoSolutions Anti-AAV Antibody
$430
PhosphoSolutions Anti-AAV4 VP3 Antibody
$430
PhosphoSolutions Anti-AAV5 VP3 Antibody
$430
Immunohistochemical staining of adult mouse retina showing specific immunolabeling of the ABCA4 protein (red, 1;100). Photo courtesy of Mary Raven, University of California, Santa Barbara, CA.Western blot of 70 day old mouse retina lysate showing immunolabeling of the ~220 kDa ABCA4 protein at a dilution of 1:1000.
PhosphoSolutions Anti-ABCA4 (Rim Protein) Antibody
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Western Blot of transfected COS-7 cell lysate showing specific immunolabeling of Acinus.
PhosphoSolutions Anti-Acinus Antibody
$430
Western Blot of transfected COS-7 cells showing specific immunolabeling of Acinus. This antibody recognizes both long and short forms of Acinus protein.
PhosphoSolutions Anti-Acinus Antibody
$430
Western Blot of testis lysate showing specific immunolabeling of FHL5.
PhosphoSolutions Anti-ACT Antibody
$430
Western blot analysis of mouse C2C12 cells probed with mouse monoclonal anti-Actin (C-terminal region) antibody at 1:1000 (lane 1), 1:2000 (lane 2), or 1:4000 (lane 3).Formalin fixed, citric acid treated parafin sections of E18 mouse skeletal muscle. Sections were probed with anti-Actin (AM2021) then anti-Mouse:HRP before detection using DAB. (Images provided by Carl Hobbs and Dr. Pat Doherty at Wolfson Centre for Age-Related Diseases, King's College London).
PhosphoSolutions Anti-Actin (C-terminal region) Antibody
$275
Western blot analysis of mouse C2C12 cells untreated (lanes 1 & 3), or treated with pervanadate (1 mM) for 30 min (lanes 2 & 4). The blot was probed with anti-Actin (N-terminal) antibody (lanes 1 & 2) or anti-Actin (Tyr-53) antibody (lanes 3 & 4).Immunocytochemical labeling using anti-Actin (N-terminal) and anti-Actin (Tyr-53) polyclonal antibodies in C2C12 cells control (left) or treated with pervanadate (1 mM) for 30 min (middle). The cells were fixed in paraformaldehyde and permeabilized in acetone. Both antibodies were used in the presence of blocking peptide: Actin (N-terminal) peptide (AX1655) or phospho-Actin (Tyr-53) peptide (AX1675), respectively (right).
PhosphoSolutions Anti-Actin (N-terminal region) Antibody
$275
Western blot analysis of mouse C2C12 cells untreated (lanes 1 & 3), or treated with pervanadate (1 mM) for 30 min (lanes 2 & 4). The blot was probed with anti-Actin (N-terminal) antibody (lanes 1 & 2) or anti-Actin (Tyr-53) antibody (lanes 3 & 4).Immunocytochemical labeling using anti-Actin (N-terminal) and anti-Actin (Tyr-53) polyclonal antibodies in C2C12 cells control (left) or treated with pervanadate (1 mM) for 30 min (middle). The cells were fixed in paraformaldehyde and permeabilized in acetone. Both antibodies were used in the presence of blocking peptide: Actin (N-terminal) peptide (AX1655) or phospho-Actin (Tyr-53) peptide (AX1675), respectively (right).
PhosphoSolutions Anti-Actin (Tyr-53), Phosphospecific Antibody
$329
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~42 kDa actin protein. Immunofluorescence staining of human blood-brain barrier endothelial cells, HMEC 3, showing specific labeling of actin (cat. 125-ACT, red, 1:200) The cell nuclei are stained in blue with DAPI. Photo credit of Yancy Ferrer-Acosta/Henrique Martins, Universidad Central Del Caribe, PR.
PhosphoSolutions Anti-Actin Antibody
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PhosphoSolutions Anti-Adenovirus 3 Antibody
$430
Immunofluorescence of a section of rat hippocampus selectively labeling the neuronal cilia with anti-adenylate cyclase III (cat. 85-AC3, 1:10,000, red), labeling nuclei with anti-MECP2 (cat. 1205-MeCP2, 1:1000,green), and Hoechst staining of nuclear DNA.Immunostaining of cultured rat neurons and glia showing strong staining of neuronal cilia using our anti-adenylate cyclase III antibody (cat. 85-AC3, 1:500, red) and axonal and dendritic staining of alpha II spectrin (cat. 98-A2SM,1:500, green) revealing the submembranous cytoskeleton and DNA (blue).
PhosphoSolutions Anti-Adenylate Cyclase III Antibody
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Western blot analysis of mouse white adipose tissue (lanes 1 & 3) and human recombinant full length adiponectin (lanes 2 & 4). The blot was probed with mouse monoclonals anti-Adiponectin (C-terminal) antibody M577 (lanes 1 & 2) or anti-Adiponectin (C-terminal) antibody M576 (lanes 3 & 4).
PhosphoSolutions Anti-Adiponectin (C-terminal region) Antibody
$329
Western blot analysis of mouse white adipose tissue (lanes 1 & 3) and human recombinant full length adiponectin (lanes 2 & 4). The blot was probed with mouse monoclonals anti-Adiponectin (C-terminal) antibody M577 (lanes 1 & 2) or anti-Adiponectin (C-terminal) antibody M576 (lanes 3 & 4).
PhosphoSolutions Anti-Adiponectin (C-terminal region) Antibody
$329
PhosphoSolutions Anti-ADNP Antibody
$430
Immunohistochemistry of rat ventral spinal cord showing specific staining of Alpha 2c Adrenergic Receptor. Western Blot of 20 ug of human brain lysate showing specific immunolabeling of Alpha 2c Adrenergic Receptor.
PhosphoSolutions Anti-Adrenergic Receptor Alpha 2c Antibody
$430
Immunofluoresence of FLAG-tagged AIF1 transfected COS cells showing AIF1 staining in red (cat. 91-AIF1, 1:50) and FLAG staining in green causing positive transfected cells to appear yellow. DNA is stained blue with DAPI.Western blot of mouse spleen lysate showing specific immunolabeling of the ~17 kDa AIF1/Iba1 protein.
PhosphoSolutions Anti-AIF1/Iba1 Antibody
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Western blot image of human jurkat cells untreated (lanes 1 & 3) or treated with calyculin A (100 nM, 30 min.) (lanes 2 and 4). The blot was probed with rabbit polyclonals anti-AIFM1 (C-terminal region) at 1:500 (lanes 1 & 2) and anti-AIFM1 (Ser-116) phospho-specific antibody at 1:1000 (lanes 3 & 4).Western blotting shows KEAP1-PGAM5-AIFM1 pathway protein level regulation by ALT. SKOV-3 cells were treated with different concentrations (0, 16, 32 and 64 μM) of ALT for 24 h. Specifically AIFM1, P-AIFM1(Ser-116) (cat. AP5501), KEAP1, Nrf2, and PGAM5 levels were detected. B-Actin used as a control. Image from publication CC-BY-4.0. PMID: 37712005
PhosphoSolutions Anti-AIFM1 (Ser-116), Phosphospecific Antibody
$329

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