Affinity purified from pooled serum. Learn more.

Tau (Ser416) Antibody

Catalog #: p1573-416 Category: Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Reactivity:
Rat, Mouse, Human, Bovine, Non-human primate, Canine
Applications:
WB 1:1000IHC 0.180555556
Species:
Rabbit
Gene Name:
MAPT
Molecular Weight:
~59, 65, 68 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1573-416, RRID:AB_2492257
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser416 of rat tau.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

Tau is a key microtubule-associated protein that plays an important role in the formation of microtubules in axons (Binder et al. 1985). Six tau isoforms have been identified as products of a single gene produced by alternative mRNA splicing (Goedert 1990). Tau mutations have been implicated in many neurodegenerative disorders such as Alzheimer’s disease (AD), Pick’s disease and progressive supranuclear palsy. It has been well documented that hyperphosphorylated tau is a major component of paired helical filaments in AD brain (Lee 1995). Serine 416 has been demonstrated to be a major phosphorylation site in vitro by CaM kinase II (Steiner at al. 1990).

Synonyms: ExpandCollapse

• AI413597 antibody
• AW045860 antibody
• DDPAC antibody
• FLJ31424 antibody
• FTDP 17 antibody
• G protein beta1/gamma2 subunit interacting factor 1 antibody
• MAPT antibody
• MAPTL antibody
• MGC134287 antibody
• MGC138549 antibody
• MGC156663 antibody
• Microtubule associated protein tau antibody
• Microtubule associated protein tau isoform 4 antibody
• Microtubule-associated protein tau antibody
• MSTD antibody
• Mtapt antibody
• MTBT1 antibody
• MTBT2 antibody
• Neurofibrillary tangle protein antibody
• Paired helical filament tau antibody
• Paired helical filament-tau antibody
• PHF tau antibody
• PHF-tau antibody
• PPND antibody
• PPP1R103 antibody
• Protein phosphatase 1, regulatory subunit 103 antibody
• pTau antibody
• RNPTAU antibody
• TAU antibody
• TAU_HUMAN antibody
• Tauopathy and respiratory failure, included antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

General References

Binder LI, Frankfurter A, Rebhun LI (1985) The distribution of tau in the mammalian central nervous system. J Cell Bio Oct; 101(4):1371-8.

Lee V.M.Y. (1995) Disruption of the cytoskeleton in Alzheimer’s disease. Curr. Opin. Neurobiol. 5, 663-668.

Goedert M. and Jakes R. (1990) Expression of separate isoforms of human tau protein: correlation with the tau pattern in brain and effects on tubulin polymerization. EMBO J 9, 4225-4230.

Steiner B., Mandelkow E.M., Biernat J. et al. (1990) Phosphorylation of microtubuleassociated protein tau: identification of the site for Ca2+-calmodulin dependent kinase and relationship with tau phosphorylation in Alzheimer tangles. EMBO J. 9, 3539-3544.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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Tau ser416 Antibody
Western blot of rat brain homogenate showing specific immunolabeling of the ~59 kDa, ~65 kDa, ~68 kDa Tau isoforms phosphorylated at Ser416 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide (not shown).
Immunostaining of formalin-fixed paraffin embedded human hippocampus showing specific immunolabeling of tau isoforms phosphorylated at Ser416 (red). Photo courtesy of Benecia Hong-Goka, Indiana Univ. SOM)

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