Affinity purified from pooled serum. Learn more.

Synaptotagmin (Thr202) Antibody

Rabbit polyclonal antibody

Catalog #: p1570-202 Categories: , Datasheet:

$119.00$380.00

  • SizePrice
Clear
Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Rat
Expected Reactivity:
Mouse, Bovine, Canine, Chicken, Human, Non-human primate, Zebra fish
Applications:
WB 1:1000ICC 1:400 Don't see your application?
Host Species:
Rabbit
Gene Name:
SYT1
Molecular Weight:
~62 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1570-202, RRID:AB_2492252
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr202 of synaptotagmin.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns

Biological Significance: ExpandCollapse

Synaptotagmin is widely regarded as the primary calcium sensor for synaptic vesicle exocytosis (Fernandez-Chacon et al., 2001; Wang et al., 2003). Moreover, recent studies indicate that the protein also plays a key role in endocytosis (Poskanzer et al., 2003). Synaptotagmin can be phosphorylated by multiple protein kinases and this may play a key role in modulation of synaptotagmin’s ability to influence both the exocytotic and endocytotic components of synaptic transmission (Hilfiker et al., 1999; Lee et al., 2004).

Synonyms: ExpandCollapse

• DKFZp781D2042 antibody
• FLJ42519 antibody
• P65 antibody
• SVP65 antibody
• synaptotagmin 1 antibody
• Synaptotagmin I antibody
• SYT antibody
• SYT1 antibody
• SytI antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

General References

Fernandez-Chacon, R., Königstorfer, A., Gerber, S.H., García, J., Matos, M.F., Stevens, C.F., Brose, N., Rizo, J., Rosenmund, C. and Südhof, T.C., 2001. Synaptotagmin I functions as a calcium regulator of release probability. Nature, 410(6824), p.41-49. PMID: 11242035

Hilfiker, S., Pieribone, V.A., Nordstedt, C., Greengard, P. and Czernik, A.J., 1999. Regulation of synaptotagmin I phosphorylation by multiple protein kinases. Journal of Neurochemistry, 73(3), pp.921-932. PMID: 10461881

Lee, B.H., Min, X., Heise, C.J., Xu, B.E., Chen, S., Shu, H., Luby-Phelps, K., Goldsmith, E.J. and Cobb, M.H., 2004. WNK1 phosphorylates synaptotagmin 2 and modulates its membrane binding. Molecular Cell, 15(5), pp.741-751. PMID: 15350218

Poskanzer, K.E., Marek, K.W., Sweeney, S.T. and Davis, G.W., 2003. Synaptotagmin I is necessary for compensatory synaptic vesicle endocytosis in vivo. Nature, 426(6966), p.559-563. PMID: 14634669

Wang, C.T., Lu, J.C., Bai, J., Chang, P.Y., Martin, T.F., Chapman, E.R. and Jackson, M.B., 2003. Different domains of synaptotagmin control the choice between kiss-and-run and full fusion. Nature, 424(6951), p.943-947. PMID: 12931189

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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