Affinity purified from pooled serum. Learn more.

Synapsin I (Ser62,67) Antibody

We are the original manufacturer of our Synapsin I (Ser62,67) rabbit polyclonal phosphospecific antibody, affinity purified from pooled serum. Excellent in WB.

Catalog #: p1560-6267 Categories: , Datasheet:

$119.00$380.00

  • SizePrice
Clear
Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Human, Mouse, Rat
Expected Reactivity:
Bovine
Applications:
WB 1:1000 Don't see your application?
Host Species:
Rabbit Polyclonal
Gene Name:
SYN1
Molecular Weight:
~78 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1560-6267, RRID:AB_2492245
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser62,67 of synapsin I.

The antibody is  prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

Synapsin I plays a key role in synaptic plasticity in brain (Feng et al., 2002; Nayak et al., 1996). This effect is due in large part to the ability of the synapsins to regulate the availability of synaptic vesicles for release. The role of synapsin in synaptic plasticity and in synaptogenesis is regulated by phosphorylation (Jovanovic et al., 2001; Kao et al., 2002). Ser549 along with Ser62 and Ser67 are the sites of Synapsin I that are phosphorylated by MAP kinase (Czernik et al., 1987; Jovanovic et al., 1996).

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Western Blot: Human | Mouse | Rat


Western Blot: Human
PMIDDilutionPublication
28052246not listedVargas, K.J., et al. 2017 Synucleins Have Multiple Effects on Presynaptic Architecture. Cell Reports, 18(1), pp.161-173.


Western Blot: Mouse
PMIDDilutionPublication
28052246not listedVargas, K.J., et al. 2017 Synucleins Have Multiple Effects on Presynaptic Architecture. Cell Reports, 18(1), pp.161-173.
19004831not listedTallent, M.K., et al. 2009 In Vivo Modulation of O-GlcNAc Levels Regulates Hippocampal Synaptic Plasticity through Interplay with Phosphorylation. Journal of Biological Chemistry, Jan 2009; 284: 174 – 181.


Western Blot: Rat
PMIDDilutionPublication
250377021:2000Chanaday, N.L., et al. 2015 Glutamate Release Machinery Is Altered in the Frontal Cortex of Rats with Experimental Autoimmune Encephalomyelitis. Molecular Neurobiology, 51(3), 1353-1367.
18519737not listedLeal-Ortiz, S., et al. 2008 Piccolo modulation of Synapsin1a dynamics regulates synaptic vesicle exocytosis. Journal of Cell Biology, 181: 831 – 846.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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