Rabbit polyclonal antibody
- Affinity Purified from Pooled Serum
- Species Reactivity:
- Rat, Mouse, Bovine
- WB 1:1000
- Gene Name:
- Molecular Weight:
- ~78 kDa
- Cite This Antibody:
- PhosphoSolutions Cat# p1560-6267, RRID:AB_2492245
The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser62,67 of synapsin I.
The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.
Synapsin I plays a key role in synaptic plasticity in brain (Feng et al., 2002; Nayak et al., 1996). This effect is due in large part to the ability of the synapsins to regulate the availability of synaptic vesicles for release. The role of synapsin in synaptic plasticity and in synaptogenesis is regulated by phosphorylation (Jovanovic et al., 2001; Kao et al., 2002). Ser549 along with Ser62 and Ser67 are the sites of Synapsin I that are phosphorylated by MAP kinase (Czernik et al., 1987; Jovanovic et al., 1996).
100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Product Specific References
Vargas, K.J., Schrod, N., Davis, T., Fernandez-Busnadiego, R., Taguchi, Y.V., Laugks, U., Lucic, V. and Chandra, S.S., 2017. Synucleins Have Multiple Effects on Presynaptic Architecture. Cell Reports, 18(1), pp.161-173. PMID: 28052246
Chanaday, N. L., Vilcaes, A. A., de Paul, A. L., Torres, A. I., Degano, A. L., & Roth, G. A. (2015). Glutamate Release Machinery Is Altered in the Frontal Cortex of Rats with Experimental Autoimmune Encephalomyelitis. Molecular neurobiology, 51(3), 1353-1367. PMID: 25037702
Melanie K. Tallent, Neal Varghis, Yuliya Skorobogatko, Lisa Hernandez-Cuebas, Kelly Whelan, David J. Vocadlo, and Keith Vosseller (2009) In Vivo Modulation of O-GlcNAc Levels Regulates Hippocampal Synaptic Plasticity through Interplay with Phosphorylation. J. Biol. Chem., Jan 2009; 284: 174 – 181. PMID: 19004831
Sergio Leal-Ortiz, Clarissa L. Waites, Ryan Terry-Lorenzo, Pedro Zamorano, Eckart D. Gundelfinger, and Craig C. Garner (2008) Piccolo modulation of Synapsin1a dynamics regulates synaptic vesicle exocytosis. J. Cell Biol., 181: 831 – 846. PMID: 18519737