Progesterone Receptor (Ser294) Antibody

Catalog #: p205-294 Category: Datasheet:

$109.00$365.00

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Mouse monoclonal antibody

Formulation:
Protein G Purified
Species Reactivity:
Human, Non-human primate
Applications:
WB 1:1000IHC 1:10
Species:
Mouse
Gene Name:
PGR
Molecular Reference:
~90 kDa and ~120 kDa
Cite This Antibody:
PhosphoSolutions Cat# p205-294, RRID:AB_2492216
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phosphoSer294 of human progesterone receptor.

The antibody is prepared from tissue culture supernatant by ammonium sulfate precipitation followed by purification using a Protein G column.

Biological Significance: ExpandCollapse

There is accumulating evidence to suggest that progesterone plays an essential role in the regulation of growth and differentiation of mammary glands and thus may play a key role in breast cancer (Edwards, 2005). The biological response to progesterone is mediated by two distinct forms of the human progesterone receptor (PR-A and PR-B forms). In most cell contexts, the B form functions as a transcriptional activator, whereas the A form functions as a transcriptional inhibitor of steroid hormones (Attia et al., 2000; Lin et al., 2003). Recently it has been demonstrated that there is differential hormone dependent regulation of the phosphorylation of the A and B forms of the receptor (Clemm et al., 2000) . Treatment of T47D breast cancer cells with progestin agonist increases the phosphorylation of Ser190 and Ser294 with different kinetics. These phosphorylation events may differentially affect the transcriptional activity of the receptor.

Synonyms: ExpandCollapse

• NR3C3 antibody
• Nuclear receptor subfamily 3 group C member 3 antibody
• PGR antibody
• PR antibody
• PRA antibody
• PRB antibody
• PRGR_HUMAN antibody
• Progesterone receptor antibody
• Progestin receptor form A antibody
• Progestin receptor form B antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.

General References

Attia GR, Zeitoun K, Edwards D, Johns A, Carr BR, Bulun SE (2000) Progesterone receptor isoform A but not B is expressed in endometriosis. J Clin Endocrinol Metab 85:2897-2902. Clemm DL, Sherman L, Boonyara tanakornkit V, Schrader WT, Weigel NL, Edwards DP (2000) Differential hormone-dependent phosphorylation of progesterone receptor A and B forms revealed by a phosphoserine site-specific monoclonal antibody. Mol Endocrinol 14:52-65.

Edwards DP (2005) Regulation of signal transduction pathways by estrogen and progesterone. Annu Rev Physiol 67:335-376. Lin VC, Woon CT, Aw SE, Guo C (2003) Distinct molecular pathways mediate progesterone-induced growth inhibition and focal adhesion. Endocrinology 144:5650-5657

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Progesterone Receptor Ser294 Antibody
Western blot of T47D cell lysate prepared from cells that had been incubated in the presence of the synthetic progestin agonist R5020 (500 nM) showing specific immunolabeling of the ~90 kDa PR-A isoform and the ~120 kDa PR-B isoform of the progesterone receptor phosphorylated at Ser294. The immunolabeling is blocked by the phosphopeptide used as the antigen (not shown).

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