Affinity purified from pooled serum. Learn more.

Potassium Chloride Cotransporter 2 (KCC2) (Thr1007) Antibody

Catalog #: p1551-1007 Category: Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Specificity:
Bovine, Human, Mouse, Non-human Primate, Pig, Rat, Sheep
Applications:
WB 1:1000
Species:
Rabbit Polyclonal
Molecular Reference:
~135 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1551-1007, RRID:AB_2716769
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr1007 of mouse KCC2.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and non-phosphopeptide affinity columns.

Biological Significance: ExpandCollapse

KCC2 is widely thought to be expressed exclusively in neurons where it is responsible for maintaining low intracellular chloride concentration to drive hyperpolarizing post-synaptic responses to the inhibitory neurotransmitters GABA and glycine (Lee et al., 2007). KCC2 is expressed in most adult neurons, and expression levels correlate well with the maturation state of neurons. N-ethylmaleimide (NEM) has been show to increase phosphorylation of the Ser940 residue, while decreasing phosphorylation of T1007 residue. Dephosphorylation of residues T906 and T1007 correlates with increased KCC2 activity (Moss et al., 2017).

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.

Product Specific References

Conway, L.C., Cardarelli, R.A., Moore, Y.E., Jones, K., McWilliams, L.J., Baker, D.J., Burnham, M.P., Bürli, R.W., Wang, Q., Brandon, N.J. and Moss, S.J., 2017. N-ethylmaleimide increases KCC2 activity by modulating transporter phosphorylation. Journal of Biological Chemistry, pp.jbc-M117.

Western blot of Potassium Choline Cotransprter Antibody
Western blot of rat hippocampal lysate showing specific labeling of the ~135 kDa KCC2 protein phosphorylated at Thr1007 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).

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