Rabbit polyclonal antibodyPooled Serum
- Affinity Purified from Pooled Serum
- Bovine, Human, Mouse, Non-human Primate, Pig, Rat, Sheep
- WB 1:1000
- Rabbit Polyclonal
- Molecular Reference:
- ~135 kDa
- Cite This Antibody:
- PhosphoSolutions Cat# p1551-1007, RRID:AB_2716769
The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr1007 of mouse KCC2.
The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and non-phosphopeptide affinity columns.
Biological Significance: ExpandCollapse
KCC2 is widely thought to be expressed exclusively in neurons where it is responsible for maintaining low intracellular chloride concentration to drive hyperpolarizing post-synaptic responses to the inhibitory neurotransmitters GABA and glycine (Lee et al., 2007). KCC2 is expressed in most adult neurons, and expression levels correlate well with the maturation state of neurons. N-ethylmaleimide (NEM) has been show to increase phosphorylation of the Ser940 residue, while decreasing phosphorylation of T1007 residue. Dephosphorylation of residues T906 and T1007 correlates with increased KCC2 activity (Moss et al., 2017).