Affinity purified from pooled serum. Learn more.

Potassium Chloride Cotransporter 2 (KCC2) (Thr1007) Antibody

We are the original manufacturer of our rabbit polyclonal Potassium Chloride Cotransporter 2 (KCC2) (Thr1007) phosphospecific antibody, affinity purified from pooled serum. It is excellent for use in WB.

Catalog #: p1551-1007 Categories: , Datasheet:

$119.00$380.00

  • SizePrice
Clear
Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Mouse, Rat
Expected Reactivity:
Bovine, Human, Non-human Primate, Pig, Sheep
Applications:
WB 1:1000 Don't see your application?
Host Species:
Rabbit Polyclonal
Gene Name:
KCC2
Molecular Weight:
~135 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1551-1007, RRID:AB_2716769
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr1007 of mouse KCC2.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and non-phosphopeptide affinity columns.

Biological Significance: ExpandCollapse

KCC2 is widely thought to be expressed exclusively in neurons where it is responsible for maintaining low intracellular chloride concentration to drive hyperpolarizing post-synaptic responses to the inhibitory neurotransmitters GABA and glycine (Lee et al., 2007). KCC2 is expressed in most adult neurons, and expression levels correlate well with the maturation state of neurons. N-ethylmaleimide (NEM) has been show to increase phosphorylation of the Ser940 residue, while decreasing phosphorylation of T1007 residue. Dephosphorylation of residues T906 and T1007 correlates with increased KCC2 activity (Moss et al., 2017).

Synonyms: ExpandCollapse

• Electroneutral potassium chloride cotransporter 2 antibody
• Electroneutral potassium-chloride cotransporter 2 antibody
• Erythroid K Cl cotransporter 2 antibody
• Furosemide sensitive K Cl cotransporter antibody
• hKCC2 antibody
• K-Cl cotransporter 2 antibody
• KCC 2 antibody
• KCC2 antibody
• KIAA1176 antibody
• Neuronal K Cl cotransporter antibody
• Neuronal K-Cl cotransporter antibody
• Potassium Chloride Cotransporter antibody
• Potassium chloride transporter 5 antibody
• rKCC2 antibody
• S12A5 antibody
• S12A5_HUMAN antibody
• SLC12A5 antibody
• Solute carrier family 12 (potassium chloride transporter) member 5 antibody
• Solute carrier family 12 member 5 antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Western Blot: Rat


Western Blot: Rat
PMID Dilution Publication
29092909 1:1000 Conway, L.C., et al. 2017 N-Ethylmaleimide increases KCC2 cotransporter activity by modulating transporter phosphorylation. Journal of Biological Chemistry, 292(52), pp.21253-21263.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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