Affinity purified from pooled serum. Learn more.

NMDA Receptor NR2B Subunit (Tyr1336) Antibody

Catalog #: p1516-1336 Categories: , Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Mouse, Rat
Expected Reactivity:
Human, Non-human primate
Applications:
WB 1:1000IHC 1:400 Don't see your application?
Species:
Rabbit
Gene Name:
GRIN2B
Molecular Weight:
~180 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1516-1336, RRID:AB_2492181
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Tyr1336 of the NR2B subunit of the rat NMDA receptor.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits (Ishii et al., 1993). Phosphorylation of Tyr1336 is thought to potentiate NMDA receptor-dependent influx of calcium (Takasu et al., 2002) and ischemia may also increase the phosphorylation of this site (Takagi et al., 2003).

Synonyms: ExpandCollapse

• AW490526 antibody
• EIEE27 antibody
• Glutamate [NMDA] receptor subunit epsilon 2 antibody
• Glutamate [NMDA] receptor subunit epsilon-2 antibody
• Glutamate Receptor Ionotropic N Methyl D Aspartate 2B antibody
• Glutamate Receptor Ionotropic N Methyl D Aspartate subunit 2B antibody
• Glutamate receptor ionotropic NMDA2B antibody
• Glutamate receptor subunit epsilon 2 antibody
• Glutamate receptor, ionotropic, NMDA2B (epsilon 2) antibody
• GRIN 2B antibody
• GRIN2B antibody
• hNR 3 antibody
• hNR3 antibody
• MGC142178 antibody
• MGC142180 antibody
• MRD6 antibody
• N methyl D asparate receptor channel subunit epsilon 2 antibody
• N methyl D aspartate receptor subtype 2B antibody
• N methyl D aspartate receptor subunit 2B antibody
• N methyl D aspartate receptor subunit 3 antibody
• N-methyl D-aspartate receptor subtype 2B antibody
• N-methyl-D-aspartate receptor subunit 3 antibody
• NMDA NR2B antibody
• NMDA R2B antibody
• NMDAR2B antibody
• NMDE2 antibody
• NMDE2_HUMAN antibody
• NME2 antibody
• NR2B antibody
• NR3 antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Immunohistochemistry: Mouse

Western Blot: Mouse | Rat


Immunohistochemistry: Mouse
PMID Dilution Publication
31235881 1:400 Pallas-Bazarra, N., et al. 2019. Tau is required for the function of extrasynaptic NMDA receptors. Scientific Reports, 9(1), p.9116.
29101031 1:400 Holmes, A., et al. 2018. A deficiency of the GluN2C subunit of the N-Methyl-d-Aspartate receptor is neuroprotective in a mouse model of ischemic stroke. Biochemical and Biophysical Research Communications. Jan 1;495(1):136-144.
28993142 1:400 Berrout, L., et al. 2018. Ghrelin upregulates the phosphorylation of the GluN2B subunit of the NMDA receptor by activating GHSR1a and Fyn in the rat hippocampus. Brain research, 1678, pp.20-26.


Western Blot: Mouse
PMID Dilution Publication
31235881 1:1000 Pallas-Bazarra, N., et al. 2019. . Tau is required for the function of extrasynaptic NMDA receptors. Scientific Reports, 9(1), p.9116.
30504838 not listed Minnella, A.M., et al. 2018. Excitotoxic superoxide production and neuronal death require both ionotropic and non-ionotropic NMDA receptor signaling. Scientific reports, 8(1), p.17522.
27094400 1:1000 Zamzow, D.R., et al. 2016. Higher levels of phosphorylated Y1472 on GluN2B subunits in the frontal cortex of aged mice are associated with good spatial reference memory, but not cognitive flexibility. AGE, 38(3), 1-17.
25158771 not listed Knox, R., et al. 2014. NR2B phosphorylation at tyrosine 1472 contributes to brain injury in a rodent model of neonatal hypoxia-ischemia. Stroke, 45(10), pp.3040-3047.
21464302 1:1000 Ticklin, T.R., et al. 2011 Alcohol inhibition of the NMDA receptor function, long-term potentiation, and fear learning requires striatal-enriched protein tyrosine phosphatase. PNAS, Apr 2011; 108: 6650 – 6655.
17687025 not listed Chernova, T., et al. 2007. Neurite Degeneration Induced by Heme Deficiency Mediated via Inhibition of NMDA Receptor-Dependent Extracellular Signal-Regulated Kinase 1/2 Activation. J. Neurosci., 27: 8475 – 8485.


Western Blot: Rat
PMID Dilution Publication
21464302 1:1000 Ticklin, T.R., et al. 2011 Alcohol inhibition of the NMDA receptor function, long-term potentiation, and fear learning requires striatal-enriched protein tyrosine phosphatase. PNAS, Apr 2011; 108: 6650 – 6655.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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NMDA Receptor NR2B Subunit tyr1336 Antibody
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2B subunit phosphorylated at Tyr1336 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).

Click here to view our Western blotting and lysate preparation protocols.
NMDA Receptor NR2B Subunit tyr1336 Antibody
Immunostaining of mouse dentate gyrus 48 hour post TMT treatment showing NR2B when phosphorylated at Tyr1336 (green, 1:400). The blue is staining nuclei using DAPI. Photo Courtesy of Rob Wine.

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