Mitochondrial Fission Factor (Mff) Antibody

Catalog #: 1157-MFF Categories: , Datasheet:

$99.00$310.00

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Rabbit polyclonal antibody

Formulation:
Neat serum
Specificity:
Bovine, Human, Mouse
Applications:
WB 1:1000IHC 1:200
Species:
Rabbit Polyclonal
Gene Name:
MFF
Molecular Reference:
~ 38 kDa
Cite This Antibody:
PhosphoSolutions Cat# 1157-MFF, RRID:AB_2736925
Antigen/Purification: ExpandCollapse

The antigen is human recombinant protein expressed in and purified from E. coli.

Biological Significance: ExpandCollapse

Mitochondrial fission factor, also known as Mff, is a tail-anchored, outer mitochondrial membrane protein that is  part of a complex process controlling mitochondrial and peroxisomal fission in conjunction with Drp1 and Fis1 (Schrader and Yoon, 2007).  The rate of mitochondrial fission and fusion balance each other for cell growth and survival of mitochondria. Fission can be greatly accelerated when cytochrome c is released during apoptosis (Desagher and Martinou, 2000). Mff was identified as an important component of the process through siRNA transfected cells, isolating the protein in the P2 pellet and demonstrating that Mff is exposed to the cytosol (Gandre-Babbe and van der Bliek 2008).  Mff has been identified at different stages of the fission process working alongside, rather than in complex with, Fis1 suggesting that Mff contributes to fission independent of the Fis1 complex (Gandre-Babbe and van der Bliek 2008).

Storage

100 µl neat serum + 0.09% NaN3

For long term storage –20° C is recommended in undiluted aliquots. Stable at –20° C for at least 1 year. Avoid freeze/thaw cycles.

Product Specific References:

Gandre-Babbe S, van der Bliek AM. The Novel Tail-anchored Membrane Protein Mff Controls Mitochondrial and Peroxisomal Fission in Mammaliam Cells. Mol Biol Cell 19(6):2402-12 (2008).

Western blot of MFF antibody
Western blot of mouse whole brain lysate showing specific immunolabeling of the ~38 kDa Mff protein.