Mitochondrial Fission Factor (Mff) Antibody

Excellent mitochondrial marker! Our Mff rabbit polyclonal antibody is neat serum. Excellent for use in WB and IHC.

Catalog #: 1157-MFF Categories: , , Datasheet:

$109.00$325.00

  • SizePrice
Clear
Formulation:
Neat serum
Species Tested:
Bovine, Human, Mouse
Applications:
WB 1:1000ICC 1:200 Don't see your application?
Host Species:
Rabbit Polyclonal
Gene Name:
MFF
Molecular Weight:
~ 38 kDa
Cite This Antibody:
PhosphoSolutions Cat# 1157-MFF, RRID:AB_2736925
Antigen/Purification: ExpandCollapse

The antigen is human recombinant protein expressed in and purified from E. coli.

Biological Significance: ExpandCollapse

Mitochondrial fission factor, also known as Mff, is a tail-anchored, outer mitochondrial membrane protein that is  part of a complex process controlling mitochondrial and peroxisomal fission in conjunction with Drp1 and Fis1 (Schrader and Yoon, 2007).  The rate of mitochondrial fission and fusion balance each other for cell growth and survival of mitochondria. Fission can be greatly accelerated when cytochrome c is released during apoptosis (Desagher and Martinou, 2000). Mff was identified as an important component of the process through siRNA transfected cells, isolating the protein in the P2 pellet and demonstrating that Mff is exposed to the cytosol (Gandre-Babbe and van der Bliek 2008).  Mff has been identified at different stages of the fission process working alongside, rather than in complex with, Fis1 suggesting that Mff contributes to fission independent of the Fis1 complex (Gandre-Babbe and van der Bliek 2008).

Synonyms: ExpandCollapse

• C2orf33 antibody
• Chromosome 2 open reading frame 33 antibody
• DKFZp666J168 antibody
• GL004 antibody
• Mff antibody
• MFF_HUMAN antibody
• MGC110913 antibody
• Mitochondrial fission factor antibody
• OTTHUMP00000164235 antibody

Storage

100 µl neat serum + 0.09% NaN3. Adequate amount of material to conduct 10-mini Western Blots.

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Product Specific Protocols

Immunocytochemistry

HeLa cells.

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Immunocytochemistry: Human
Western Blot: Human


Immunocytochemistry: Human
PMID Dilution Publication
18353969 1:200 Gandre-Babbe, S., et al. 2008 The novel tail-anchored membrane protein Mff controls mitochondrial and peroxisomal fission in mammalian cells. Molecular Biology of the Cell, 19(6), pp.2402-2412.


Western Blot: Human
PMID Dilution Publication
18353969 not listed Gandre-Babbe, S., et al. 2008 The novel tail-anchored membrane protein Mff controls mitochondrial and peroxisomal fission in mammalian cells. Molecular Biology of the Cell, 19(6), pp.2402-2412.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
Leave a Reply

Your email address will not be published. Required fields are marked *

This site uses Akismet to reduce spam. Learn how your comment data is processed.

You may also like…