Our Anti-Mitochondrial Fission Factor (Mff) primary antibody from PhosphoSolutions is rabbit polyclonal. It detects bovine, human, and mouse Mitochondrial Fission Factor (Mff) and is neat serum. It is great for use in WB, ICC.
Western blot of mouse whole brain lysate showing specific immunolabeling of the ~38 kDa Mff protein.
Mitochondrial fission factor, also known as Mff, is a tail-anchored, outer mitochondrial membrane protein that is part of a complex process controlling mitochondrial and peroxisomal fission in conjunction with Drp1 and Fis1 (Schrader and Yoon, 2007). The rate of mitochondrial fission and fusion balance each other for cell growth and survival of mitochondria. Fission can be greatly accelerated when cytochrome c is released during apoptosis (Desagher and Martinou, 2000). Mff was identified as an important component of the process through siRNA transfected cells, isolating the protein in the P2 pellet and demonstrating that Mff is exposed to the cytosol (Gandre-Babbe and van der Bliek 2008). Mff has been identified at different stages of the fission process working alongside, rather than in complex with, Fis1 suggesting that Mff contributes to fission independent of the Fis1 complex (Gandre-Babbe and van der Bliek 2008).
Neat Serum
Polyclonal
IgG
ICC, WB
Rabbit
MFF
38 kDa
Human recombinant protein
Bovine, Human, Mouse
AB_2736925
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
Liquid
Neat sera
Neat serum + 0.09% NaN3
WB: 1:1000
WB Brain: 1:1000
ICC: 1:200
Unconjugated
Specific for endogenous levels of the ~38 kDa Mff protein.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Gandre-Babbe, S., et al. 2008. The novel tail-anchored membrane protein Mff controls mitochondrial and peroxisomal fission in mammalian cells. Molecular Biology of the Cell, 19(6), pp.2402-2412.
Gandre-Babbe, S., et al. 2008. The novel tail-anchored membrane protein Mff controls mitochondrial and peroxisomal fission in mammalian cells. Molecular Biology of the Cell, 19(6), pp.2402-2412.
Bulk Order Anti-Mitochondrial Fission Factor (Mff) Antibody
