Mitochondria Antibody

Our mitochondria antibody reacts specifically with human cells. It is mouse monoclonal and is Protein A purified. Excellent for use in WB, IHC, and IF.

Catalog #: 1155-MIT Categories: , , , Datasheet:


  • SizePrice
Protein A purified
Species Tested:
WB 1:1000IHC 1:100-1:800ICC 1:100-1:800 Don't see your application?
Host Species:
Mouse Monoclonal
Molecular Weight:
~60 kDa
Cite This Antibody:
PhosphoSolutions Cat# 1155-MIT, RRID:AB_2492154
Antigen/Purification: ExpandCollapse

The antigen is a human cell homogenate.

The antibody is Protein A purified.

Biological Significance: ExpandCollapse

Mitochondria are most commonly known as the power plants of the cell as they produce ATP, but they are also involved in many other important cellular processes such as cell signaling, growth and differentiation (McBride et al., 2006). In addition, mitochondria have been shown to play a role in apoptosis (Green 1998). This antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells.


100 µl liquid. Adequate amount of material to conduct 10-mini Western Blots.

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Product Specific Protocols


HeLa Cells.

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

General References

McBride HM, Neuspiel M, Wasiak S (2006) Mitochondria: More than just a powerhouse. Curr Biol., 16(14):R551-60. PMID: 16860735

Green DR (1998) Apoptotic pathways: the roads to ruin. Cell, 94(6):695-8. PMID: 9753316

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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