Mitochondria Antibody

Catalog #: 1155-MIT Categories: , , Datasheet:

$99.00$310.00

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Reacts specifically with human cells.

 

Formulation:
Protein A purified
Species Tested:
Human
Applications:
WB 1:1000IHC 1:100-1:800ICC 1:100-1:800 Don't see your application?
Species:
Mouse monoclonal
Molecular Weight:
~60 kDa
Cite This Antibody:
PhosphoSolutions Cat# 1155-MIT, RRID:AB_2492154
Antigen/Purification: ExpandCollapse

The antigen is a human cell homogenate.

The antibody is Protein A purified.

Biological Significance: ExpandCollapse

Mitochondria are most commonly known as the power plants of the cell as they produce ATP, but they are also involved in many other important cellular processes such as cell signaling, growth and differentiation (McBride et al., 2006). In addition, mitochondria have been shown to play a role in apoptosis (Green 1998). This antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells.

Storage

100 µl liquid. Adequate amount of material to conduct 10-mini Western Blots.

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Product Specific Protocols

Immunocytochemistry

HeLa Cells.

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

General References

McBride HM, Neuspiel M, Wasiak S (2006) Mitochondria: More than just a powerhouse. Curr Biol., 16(14):R551-60. PMID: 16860735

Green DR (1998) Apoptotic pathways: the roads to ruin. Cell, 94(6):695-8. PMID: 9753316

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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Mitochondria Antibody
Western blot of HeLa cell lysate showing specific immunolabeling of the ~ 60 kDa mitochondrial protein.

Click here to view our Western blotting and lysate preparation protocols.
Mitochondria Antibody
Immunofluorescence of HeLa cells labeled with anti-mitochondria antibody(1:100, green).
Download the protocol used for this image:

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