Affinity purified from pooled serum. Learn more.

GABAB R2 (Ser783) Antibody

Catalog #: p1148-783 Category: Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Mouse, Rat
Species Reactivity:
Bovine, Canine, Chicken, Human, Non-human primate, Xenopus
Applications:
WB 1:1000IHC 1:500ICC 1:500
Species:
Rabbit
Gene Name:
GABBR2
Molecular Weight:
~102 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1148-783, RRID:AB_2492117
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser783 of GABAB.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system. There are two major classes of GABA receptors: the GABAA and the GABAB subtype of receptors. GABAB receptors are heterodimeric G protein-coupled receptors that mediate slow synaptic inhibition in the central nervous system. It has recently been demonstrated that AMPK binds directly to GABAB receptors and phosphorylates S783 in the cytoplasmic tail of the R2 subunit and that S783 plays a critical role in enhancing neuronal survival after ischemia as phosphorylation of S783 is evident in many brain regions and is increased dramatically after ischemic injury to the brain (Kuramoto et al., 2007).

Synonyms: ExpandCollapse

• BcDNA:GH07312 antibody
• CG6706 antibody
• CT20836 antibody
• D Gaba2 antibody
• FLJ36928 antibody
• G protein coupled receptor 51 antibody
• G-protein coupled receptor 51 antibody
• GAB B R2 antibody
• GABA B R2 antibody
• GABA B receptor 2 antibody
• GABA-B receptor 2 antibody
• GABA-B-R2 antibody
• GABA-BR2 antibody
• GABAB R2 antibody
• GABABR 2 antibody
• GABABR2 antibody
• GABB R2 antibody
• GABBR 2 antibody
• Gabbr2 antibody
• GABR2_HUMAN antibody
• Gamma aminobutyric acid B receptor 2 antibody
• Gamma aminobutyric acid GABA B receptor 2 antibody
• Gamma aminobutyric acid type B receptor subunit 2 antibody
• Gamma-aminobutyric acid type B receptor subunit 2 antibody
• Gb 2 antibody
• Gb2 antibody
• GH07312 antibody
• GPR 51 antibody
• GPR51 antibody
• GPRC 3B antibody
• GPRC3B antibody
• HG 20 antibody
• HG20 antibody
• HRIHFB2099 antibody
• Metabotropic GABA B receptor subtype 2 antibody
• OTTHUMP00000021776 antibody
• OTTHUMP00000063797 antibody
• R2 SUBUNIT antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Immunocytochemistry: Rat
Immunohistochemistry: Rat
Western Blot: Mouse | Rat


Immunocytochemistry: Rat
PMID Dilution Publication
17224405 1:500 Kuramoto, N., et al. 2007. Phospho-dependent functional modulation of GABA(B) receptors by the metabolic sensor AMP-dependent protein kinase. Neuron, 53:233-247.


Immunohistochemistry: Rat
PMID Dilution Publication
17224405 1:500 Kuramoto, N., et al. 2007. Phospho-dependent functional modulation of GABA(B) receptors by the metabolic sensor AMP-dependent protein kinase. Neuron, 53:233-247.


Western Blot: Mouse
PMID Dilution Publication
25843643 1:200 Marron Fernandez de Velasco, E., et al. 2015. Sex differences in GABA B R-GIRK signaling in layer 5/6 pyramidal neurons of the mouse prelimbic cortex. Neuropharmacology. Aug;95:353-60.
25261019 1:500 Terunuma, M., et al. 2015. Purinergic receptor activation facilitates astrocytic GABAB receptor calcium signaling. Neuropharmacology 88: 74-81.
24431439 1:500 Terunuma, M., et al. 2014. Postsynaptic GABAB receptor activity regulates excitatory neuronal architecture and spatial memory. J Neurosci. 34: 804-816.
22405207 1:500 Padgett, C.L., et al. 2012. Postsynaptic GABAB receptor activity regulates excitatory neuronal architecture and spatial memory. J Neurosci. 34: 804-816.


Western Blot: Rat
PMID Dilution Publication
29079139 1:1000 Kim, S.H., et al. 2018. The antipsychotic agent clozapine induces autophagy via the AMPK-ULK1-Beclin1 signaling pathway in the rat frontal cortex. Progress in Neuro-Psychopharmacology and Biological Psychiatry. Feb 2;81:96-104.
20643948 1:500 Terunuma, M., et al. 2010. Prolonged activation of NMDA receptors promotes dephosphorylation and alters postendocytic sorting of GABAB receptors. Proc Natl Acad Sci USA 107: 13918-13923.
17224405 1:500 Kuramoto, N., et al. 2007. Phospho-dependent functional modulation of GABA(B) receptors by the metabolic sensor AMP-dependent protein kinase. Neuron, 53:233-247.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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GABA B R2 ser783 Antibody
Western blot of rat synaptic membrane lysate showing specific immunolabeling of the ~102 kDa GABAB R2 protein phosphorylated at Ser783 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).

Click here to view our Western blotting and lysate preparation protocols.

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