Affinity purified from pooled serum. Learn more.

GABAA Receptor α2 Antibody

Catalog #: 822-GA2CL** Category: Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

**This antibody is a replacement for our original catalog #822-GA2C. It was
produced by the same methods, using the same fusion protein antigen
in new animals.

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Reactivity:
Rat, Mouse, Human, Bovine, Non-human primate, Zebra fish, Canine
Applications:
WB 1:1000ICC 1:1000
Species:
Rabbit
Gene Name:
GABRA2
Molecular Weight:
~51 kDa
Cite This Antibody:
PhosphoSolutions Cat# 822-GA2CL, RRID:AB_2492101
Antigen/Purification: ExpandCollapse

The antigen is a fusion protein from the cytoplasmic loop of the ?2-subunit of rat GABAA receptor.

The antibody is prepared from rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled.

Biological Significance: ExpandCollapse

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl− channelassociated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six αs, four βs and four γs, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for α- and β-subunitsresults in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a γ-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different ?- subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Pöltl et al., 2003).

Synonyms: ExpandCollapse

• GABA A receptor subunit alpha 2 antibody
• GABA antibody
• GABA(A) receptor subunit alpha 2 antibody
• GABA(A) receptor subunit alpha-2 antibody
• GABR A2 antibody
• GABRA 2 antibody
• GABRA2 antibody
• GABRA2 protein antibody
• Gamma aminobutyric acid (GABA) A receptor alpha 2 antibody
• Gamma aminobutyric acid A receptor alpha 2 antibody
• Gamma aminobutyric acid receptor alpha 2 subunit antibody
• Gamma aminobutyric acid receptor subunit alpha 2 antibody
• Gamma-aminobutyric acid receptor subunit alpha-2 antibody
• GBRA2_HUMAN antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific References for Applications and Species

Immunohistochemistry: Mouse
Immunocytochemistry: Mouse
Western Blot: Mouse


Immunocytochemistry: Mouse
PMID Dilution Publication
27044742*** 1:1000 Nakamura, Y., et al. 2016. Proteomic characterization of inhibitory synapses using a novel pHluorin-tagged γ-aminobutyric acid receptor, type A (GABAA), α2 subunit knock-in mouse. Journal of Biological Chemistry, 291(23), pp.12394-12407.


Immunohistochemistry: Mouse
PMID Dilution Publication
26446222*** not listed Engin, E., et al. 2015. Tonic Inhibitory Control of Dentate Gyrus Granule Cells by α5-Containing GABAA Receptors Reduces Memory Interference. The Journal of Neuroscience, 35(40), 13698-13712.


Western blot: Mouse
PMID Dilution Publication
30984232 1:500 Mulligan, M.K., et al. 2019. Identification of a functional non-coding variant in the GABAA receptor α2 subunit of the C57BL/6J mouse reference genome: Major implications for neuroscience research. Frontiers in genetics, 10, p.188

***Product specific references for previous product # 822-GA2C which has been depleted and replaced with our product # 822-GA2CL which was produced by the same methods, using the same fusion protein antigen in new animals.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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GABA A Receptor alpha 2 Antibody
Western blot of mouse brain lysates from wild type (WT) and α2-knockout (K/O) animals showing specific immunolabeling of the ~51 kDa γ2-subunit of the GABAA-R. The labeling was absent from a lysate prepared from α2-knockout animals.

Click here to view our Western blotting and lysate preparation protocols.

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