Affinity purified from pooled serum. Learn more.

GABAA Receptor α2 Antibody

Our rabbit polyclonal GABAA Receptor α2 total protein antibody is affinity purified from pooled serum. It is excellent for use in WB and ICC.

Catalog #: 822-GA2CL** Categories: , Datasheet:

$119.00$380.00

  • SizePrice
Clear

**This antibody is a replacement for our original catalog #822-GA2C. It was produced by the same methods, using the same fusion protein antigen in new animals.

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Mouse
Expected Reactivity:
Rat, Human, Bovine, Non-human primate, Zebra fish, Canine
Applications:
WB 1:1000ICC 1:1000 Don't see your application?
Host Species:
Rabbit Polyclonal
Gene Name:
GABRA2
Molecular Weight:
~51 kDa
Cite This Antibody:
PhosphoSolutions Cat# 822-GA2CL, RRID:AB_2492101
Antigen/Purification: ExpandCollapse

The antigen is a fusion protein from the cytoplasmic loop of the α2-subunit of rat GABAA receptor.

The antibody is prepared from rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled.

Biological Significance: ExpandCollapse

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl− channelassociated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six αs, four βs and four γs, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for α- and β-subunitsresults in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a γ-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different α- subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Pöltl et al., 2003).

Synonyms: ExpandCollapse

• GABA A receptor subunit alpha 2 antibody
• GABA antibody
• GABA(A) receptor subunit alpha 2 antibody
• GABA(A) receptor subunit alpha-2 antibody
• GABR A2 antibody
• GABRA 2 antibody
• GABRA2 antibody
• GABRA2 protein antibody
• Gamma aminobutyric acid (GABA) A receptor alpha 2 antibody
• Gamma aminobutyric acid A receptor alpha 2 antibody
• Gamma aminobutyric acid receptor alpha 2 subunit antibody
• Gamma aminobutyric acid receptor subunit alpha 2 antibody
• Gamma-aminobutyric acid receptor subunit alpha-2 antibody
• GBRA2_HUMAN antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Immunohistochemistry: Mouse
Immunocytochemistry: Mouse
Western Blot: Mouse


Immunocytochemistry: Mouse
PMID Dilution Publication
***27044742 1:1000 Nakamura, Y., et al. 2016. Proteomic characterization of inhibitory synapses using a novel pHluorin-tagged γ-aminobutyric acid receptor, type A (GABAA), α2 subunit knock-in mouse. Journal of Biological Chemistry, 291(23), pp.12394-12407.


Immunohistochemistry: Mouse
PMID Dilution Publication
***26446222 not listed Engin, E., et al. 2015. Tonic Inhibitory Control of Dentate Gyrus Granule Cells by α5-Containing GABAA Receptors Reduces Memory Interference. The Journal of Neuroscience, 35(40), 13698-13712.


Western blot: Mouse
PMID Dilution Publication
32620552 1:500 Nakamura, Y., et al. 2020. Phosphorylation on Ser 359 of the α2 subunit in GABA type A receptors down-regulates their density at inhibitory synapses. Journal of Biological Chemistry, pp.jbc-RA120.
30984232 1:500 Mulligan, M.K., et al. 2019. Identification of a functional non-coding variant in the GABAA receptor α2 subunit of the C57BL/6J mouse reference genome: Major implications for neuroscience research. Frontiers in genetics, 10, p.188

***Product specific references for previous product # 822-GA2C which has been depleted and replaced with our product # 822-GA2CL which was produced by the same methods, using the same fusion protein antigen in new animals.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
Leave a Reply

Your email address will not be published. Required fields are marked *

This site uses Akismet to reduce spam. Learn how your comment data is processed.

You may also like…