Affinity purified from pooled serum. Learn more.

GABA_A Receptor α₂ Antibody

Name: GABA A Receptor α 2 Antibody
SKU: 822-GA2CL*
Price: 365 USD
Availability: InStock

Catalog #: 822-GA2CL* Category: GABA Receptors Datasheet:

$109.00 – $365.00

Rabbit polyclonal antibody

*This antibody is a replacement for our original catalog #822-GA2C. It was
produced by the same methods, using the same fusion protein antigen
in new animals.

Pooled Serum

Formulation:: Affinity Purified from Pooled Serum
Species Reactivity:: Rat, Mouse, Human, Bovine, Non-human primate, Zebra fish, Canine
Applications:: WB 1:1000
Species:: Rabbit
Gene Name:: GABRA2
Molecular Reference:: ~51 kDa
Cite This Antibody:: PhosphoSolutions Cat# 822-GA2CL, RRID:AB_2492101

Antigen/Purification: ExpandCollapse

The antigen is a fusion protein from the cytoplasmic loop of the ?2-subunit of rat GABA_A receptor.

The antibody is prepared from rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled.

Biological Significance: ExpandCollapse

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl? channel associated with the GABA_A receptor (GABA_A-R) subtype. GABA_A-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABA_A-R is a multimeric subunit complex. To date six ?s, four ?s and four ?s, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for ?- and ?-subunits results in the expression of functional GABA_A-Rs sensitive to GABA. However, coexpression of a ?-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different ?- subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Pöltl et al., 2003).

Synonyms: ExpandCollapse

• GABA A receptor subunit alpha 2 antibody
• GABA antibody
• GABA(A) receptor subunit alpha 2 antibody
• GABA(A) receptor subunit alpha-2 antibody
• GABR A2 antibody
• GABRA 2 antibody
• GABRA2 antibody
• GABRA2 protein antibody
• Gamma aminobutyric acid (GABA) A receptor alpha 2 antibody
• Gamma aminobutyric acid A receptor alpha 2 antibody
• Gamma aminobutyric acid receptor alpha 2 subunit antibody
• Gamma aminobutyric acid receptor subunit alpha 2 antibody
• Gamma-aminobutyric acid receptor subunit alpha-2 antibody
• GBRA2_HUMAN antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific References

Hawkins, N.A., Zachwieja, N.J., Miller, A.R., Anderson, L.L. and Kearney, J.A., 2016. Fine mapping of a Dravet syndrome modifier locus on mouse chromosome 5 and candidate gene analysis by RNA-seq. PLoS Genet., 12(10):e1006398. PMID: 27768696

Engin, E., Zarnowska, E. D., Benke, D., Tsvetkov, E., Sigal, M., Keist, R., Bolshakov, V.Y., Pearce, R.A., & Rudolph, U. (2015). Tonic Inhibitory Control of Dentate Gyrus Granule Cells by ?5-Containing GABAA Receptors Reduces Memory Interference. The Journal of Neuroscience, 35(40), 13698-13712. PMID: 26446222

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Western blot of mouse brain lysates from wild type (WT) and ?2-knockout (K/O) animals showing specific immunolabeling of the ~51 kDa ?2-subunit of the GABA_A-R. The labeling was absent from a lysate prepared from ?2-knockout animals.

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