FOX1 Antibody

Catalog #: 581-FOX1 Categories: , , , Datasheet:

$119.00$380.00

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Excellent marker of neurons and neuronal stem cells!

 

Formulation:
Protein G Purified
Species Tested:
Bovine, Human, Mouse, Rat
Applications:
WB 1:5000IHC 1:500ICC 1:500 Don't see your application?
Species:
Mouse monoclonal
Gene Name:
RBFOX1
Molecular Weight:
~46/48 kDa
Cite This Antibody:
PhosphoSolutions Cat# 581-FOX1, RRID:AB_2492095
Antigen/Purification: ExpandCollapse

The antigen is a recombinant protein taken from the N-terminus of human FOX1 expressed in and purified from E. Coli.

The antibody is Protein G purified from culture supernatant.

Biological Significance: ExpandCollapse

FOX1, also known as ataxin-2 binding protein 1 (A2BP1), is a neuron specific RNA-binding protein that has been shown to regulate a wide range of alternative splicing events implicated in neuronal development and maturation including transcription factors and synaptic proteins (Fogel et al., 2012). Defects in FOX1 have also been associated with autism (Martin et al., 2007), attention-deficit hyperactivity disorder (Elia et al., 2010) and schizophrenia (Xu et al., 2008).

Synonyms: ExpandCollapse

• 2BP1 antibody
• A2 BP1 antibody
• A2BP 1 antibody
• A2BP antibody
• A2BP1 antibody
• Ataxin 2 binding protein 1 antibody
• Ataxin-2-binding protein 1 antibody
• Ataxin2 binding protein 1 antibody
• FOX1 antibody
• Fox-1 homolog A antibody
• fox-1-like RNA-binding protein 1 antibody
• Hexaribonucleotide binding protein 1 antibody
• Hexaribonucleotide-binding protein 1 antibody
• HRNBP 1 antibody
• HRNBP1 antibody
• RBFOX1 antibody
• RFOX1_HUMAN antibody
• RNA binding protein fox-1 homolog 1 antibody
• RNA binding protein fox-1 homolog 1, C. elegans, homolog of, 1 antibody
• RNA binding protein, fox-1 homolog (C. elegans) 1 antibody

Storage

100 µl in PBS + 10 mM sodium azide. Adequate amount of material to conduct 10-mini Western Blots.

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Product Specific Protocols

Immunocytochemistry

Cultured rat neurons

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

General References

Elia, J., Gai, X., Xie, H.M., Perin, J.C., Geiger, E., Glessner, J.T., D’arcy, M., Deberardinis, R., Frackelton, E., Kim, C. and Lantieri, F., 2010. Rare structural variants found in attention-deficit hyperactivity disorder are preferentially associated with neurodevelopmental genes. Molecular Psychiatry, 15(6), p.637-46. PMID: 19546859

Fogel, B.L., Wexler, E., Wahnich, A., Friedrich, T., Vijayendran, C., Gao, F., Parikshak, N., Konopka, G. and Geschwind, D.H., 2012. RBFOX1 regulates both splicing and transcriptional networks in human neuronal development. Human Molecular Genetics, 21(19), pp.4171-4186. PMID: 22730494

Martin, C.L., Duvall, J.A., Ilkin, Y., Simon, J.S., Arreaza, M.G., Wilkes, K., Alvarez‐Retuerto, A., Whichello, A., Powell, C.M., Rao, K. and Cook, E., 2007. Cytogenetic and molecular characterization of A2BP1/FOX1 as a candidate gene for autism. American Journal of Medical Genetics Part B: Neuropsychiatric Genetics, 144(7), pp.869-876. PMID: 17503474

Xu, B., Roos, J.L., Levy, S., Van Rensburg, E.J., Gogos, J.A. and Karayiorgou, M., 2008. Strong association of de novo copy number mutations with sporadic schizophrenia. Nature Genetics, 40(7), p.880-5. PMID: 18511947

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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