FOX1 Antibody

Catalog #: 581-FOX1 Categories: , , Datasheet:

$109.00$365.00

  • SizePrice
Clear

Excellent marker of neurons and neuronal stem cells!

 

Formulation:
Protein G Purified
Species Tested:
Bovine, Human, Mouse, Rat
Applications:
WB 1:5000IHC 1:500ICC 1:500
Species:
Mouse monoclonal
Gene Name:
RBFOX1
Molecular Weight:
~46/48 kDa
Cite This Antibody:
PhosphoSolutions Cat# 581-FOX1, RRID:AB_2492095
Antigen/Purification: ExpandCollapse

The antigen is a recombinant protein taken from the N-terminus of human FOX1 expressed in and purified from E. Coli.

The antibody is Protein G purified from culture supernatant.

Biological Significance: ExpandCollapse

FOX1, also known as ataxin-2 binding protein 1 (A2BP1), is a neuron specific RNA-binding protein that has been shown to regulate a wide range of alternative splicing events implicated in neuronal development and maturation including transcription factors and synaptic proteins (Fogel et al., 2012). Defects in FOX1 have also been associated with autism (Martin et al., 2007), attention-deficit hyperactivity disorder (Elia et al., 2010) and schizophrenia (Xu et al., 2008).

Synonyms: ExpandCollapse

• 2BP1 antibody
• A2 BP1 antibody
• A2BP 1 antibody
• A2BP antibody
• A2BP1 antibody
• Ataxin 2 binding protein 1 antibody
• Ataxin-2-binding protein 1 antibody
• Ataxin2 binding protein 1 antibody
• FOX1 antibody
• Fox-1 homolog A antibody
• fox-1-like RNA-binding protein 1 antibody
• Hexaribonucleotide binding protein 1 antibody
• Hexaribonucleotide-binding protein 1 antibody
• HRNBP 1 antibody
• HRNBP1 antibody
• RBFOX1 antibody
• RFOX1_HUMAN antibody
• RNA binding protein fox-1 homolog 1 antibody
• RNA binding protein fox-1 homolog 1, C. elegans, homolog of, 1 antibody
• RNA binding protein, fox-1 homolog (C. elegans) 1 antibody

Storage

100 µl in PBS + 10 mM sodium azide. Adequate amount of material to conduct 10-mini Western Blots.

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Product Specific Protocols

Immunocytochemistry

Cultured rat neurons

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

General References

Elia J, Gai X, Xie HM, Perin JC, Geiger E, Glessner JT, D’arcy M, deBerardinis R, Frackelton E, Kim C, Lantieri F, Muganga BM, Wang L, Takeda T, Rappaport EF, Grant SF, Berrettini W, Devoto M, Shaikh TH, Hakonarson H, White PS. (2010) Rare structural variants found in attention-deficit hyperactivity disorder are preferentially associated with neurodevelopmental genes. Mol Psychiatry. Jun;15(6):637-46.

Fogel BL, Wexler E, Wahnich A, Friedrich T, Vijayendran C, Gao F, Parikshak N, Konopka G, Geschwind DH. (2012) RBFOX1 regulates both splicing and transcriptional networks in human neuronal development. Hum Mol Genet. Oct 1;21(19):4171-86.

Martin CL, Duvall JA, Ilkin Y, Simon JS, Arreaza MG, Wilkes K, Alvarez-Retuerto A, Whichello A, Powell CM, Rao K, Cook E, Geschwind DH. (2007) Cytogenetic and molecular characterization of A2BP1/FOX1 as a candidate gene for autism. Am J Med Genet B Neuropsychiatr Genet. Oct 5;144B(7):869-76.

Xu B, Roos JL, Levy S, van Rensburg EJ, Gogos JA, Karayiorgou M. (2008) Strong association of de novo copy number mutations with sporadic schizophrenia. Nat Genet. Jul;40(7):880-5.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
Leave a Reply

Your email address will not be published. Required fields are marked *

This site uses Akismet to reduce spam. Learn how your comment data is processed.

FOX1 Antibody
Western blot of rat cortical lysate showing specific immunolabeling of the ~46/48 kDa FOX1 protein.

Click here to view our Western blotting and lysate preparation protocols.
Immunofluorescence of cultured rat neurons showing nuclear and distal cytoplasmic labeling of FOX1 (cat. # 581-FOX1, 1:500, green).

Download the protocol used for this image:

You may also like…