Affinity purified from pooled serum. Learn more.

FANCI (Ser559) Antibody

Catalog #: p171-559 Categories: , , Datasheet:

$119.00$380.00

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Rabbit Polyclonal Antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Human
Expected Reactivity:
Non-human primate
Applications:
WB 1:250 Don't see your application?
Species:
Rabbit Polyclonal
Gene Name:
FANCI
Molecular Weight:
~150 kDa
Cite This Antibody:
PhosphoSolutions Cat# p171-559, RRID:AB_2636808
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho Ser559 of human FANCI.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.

Biological Significance: ExpandCollapse

Fanconi anemia, FA, is a rare disorder where cells cannot prevent, repair, or tolerate DNA damage, leading to cancer, progressive bone marrow failure and developmental abnormalities (Ishiai et al., 2008).  16 genes have been implicated in FA, and their products constitute a common FA pathway (Walden and Deans 2014).  FANCI (Fanconi anemia complementation group I), is one of two substrates for monoubiquitnation by the FANCL-containing core complex, and is crucial for DNA repair via FAP and intrastrand cross-links (Walden and Deans, 2014). Phosphorylation of ser556 and ser559 are highly conserved and have been determined to be ATM/ATR kinase substrates for the FA-DNA pathway (Smogorzewska et al., 2007).  Furthermore, the phosphorylation of ser556 and ser559 have been shown to play key roles in FANCI physically associating with FANCD2, stabilizing the ID2 complex, and supporting its DNA binding and subsequent D2-ubiquitination (Walden and Deans, 2014).  Research continues to determine if one site plays more of a role in the DNA repair/damage checkpoint of the FA pathway and stabilization of the ID2 complex.

Synonyms: ExpandCollapse

• FANCI antibody
• FANCI gene antibody
• FANCI_HUMAN antibody
• Fanconi anemia group I protein antibody
• Fanconi anemia, complementation group I antibody
• FLJ10719 antibody
• FLJ14658 antibody
• KIAA1794 antibody
• Protein FACI antibody
• Protein FANCI antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Western Blot: Human


Western Blot: Human
PMID Dilution Publication
28636932 not listed Cheung, R., et al. 2017 Ubiquitination-linked phosphorylation of the FANCI S/TQ cluster contributes to activation of the Fanconi anemia I/D2 complex. Cell Reports, 19(12), pp.2432-2440.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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