Affinity purified from pooled serum. Learn more.

FANCI (Ser559) Antibody

Catalog #: p171-559 Categories: , Datasheet:

$109.00$365.00

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Rabbit Polyclonal Antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Specificity:
Human, Non-human primate
Applications:
WB 1:250
Species:
Rabbit Polyclonal
Gene Name:
FANCI
Molecular Reference:
~150 kDa
Cite This Antibody:
PhosphoSolutions Cat# p171-559, RRID:AB_2636808
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho Ser559 of human FANCI.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.

Biological Significance: ExpandCollapse

Fanconi anemia, FA, is a rare disorder where cells cannot prevent, repair, or tolerate DNA damage, leading to cancer, progressive bone marrow failure and developmental abnormalities (Ishiai et al., 2008).  16 genes have been implicated in FA, and their products constitute a common FA pathway (Walden and Deans 2014).  FANCI (Fanconi anemia complementation group I), is one of two substrates for monoubiquitnation by the FANCL-containing core complex, and is crucial for DNA repair via FAP and intrastrand cross-links (Walden and Deans, 2014). Phosphorylation of ser556 and ser559 are highly conserved and have been determined to be ATM/ATR kinase substrates for the FA-DNA pathway (Smogorzewska et al., 2007).  Furthermore, the phosphorylation of ser556 and ser559 have been shown to play key roles in FANCI physically associating with FANCD2, stabilizing the ID2 complex, and supporting its DNA binding and subsequent D2-ubiquitination (Walden and Deans, 2014).  Research continues to determine if one site plays more of a role in the DNA repair/damage checkpoint of the FA pathway and stabilization of the ID2 complex.

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.

General References

Ishiai M, Kitao H, Smogorzewska A, Tomida J, Kinomura A, Uchida E, Saberi A, Kinoshita E, Kinoshita-Kikuta E, Koike T, Tashiro S, Elledge S, Takata M (2008) FANCI phosphorylation functions as a molecular switch to turn on the Fanconi anemia pathway. Nat Struct Mol Biol Nov;15(11):1138-46.

Walden H, Deans A (2014) The Fanconi Anemia DNA Repair Pathway: Structural and Functional Insights into a Complex Disorder. Annu Rev Biophys 2014;43:257-78.

Smogorzewska A, Matsuoka S, Vinciguerra P, McDonald ER 3rd, Hurov KE, Luo J, Ballif BA, Gugi SP, Hofmann K, D’Andrea AD, Elledge SJ (2007) Identification of the Fanconi anemia (FANC) I protein, a monoubiquitinated FANCD2 paralog required for crosslink repair. Cell 129(2):289-301.

 

 

FANCI ser559 antibody
Western blot of HeLa cell lysates that had been treated with UV (~254 nm) for 0’ or 20’ showing specific immunolabeling of the ~150 kDa FANCI protein phosphorylated at Ser559.

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