Rabbit polyclonal antibodyPooled Serum
- Affinity Purified from Pooled Serum
- 100% Homology:
- Human, Bovine, Non-human primate, Sheep, Goat
- WB 1:1,000
- Gene Name:
- Molecular Reference:
- ~150 kDa
- Cite This Antibody:
- PhosphoSolutions Cat# p171-556, RRID:AB_2492092
The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho Ser556 of human FANCI.
The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.
Biological Significance: ExpandCollapse
Fanconi anemia, FA, is a rare disorder where cells cannot prevent, repair, or tolerate DNA damage, leading to cancer, progressive bone marrow failure and developmental abnormalities (Ishiai et al, 2008). 16 genes have been implicated in FA, and their products constitute a common FA pathway (Walden and Deans 2014). FANCI (Fanconi anemia complementation group I), is one of two substrates for monoubiquitination by the FANCL-containing core complex, and is crucial for DNA repair via FAP and intrastrand cross-links (Walden and Deans, 2014). Phosphorylation at Ser556 has been shown to play a key role in FANCI physically associating with FANCD2, stabilizing the ID2 complex, and supporting its DNA binding and subsequent D2-ubiquitination (Walden and Deans, 2014).