Affinity purified from pooled serum. Learn more.

FANCI (Ser556) Antibody

Catalog #: p171-556 Categories: , Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Reactivity:
Human, Bovine, Non-human primate, Sheep, Goat
Applications:
WB 1:1000
Species:
Rabbit
Gene Name:
FANCI
Molecular Weight:
~150 kDa
Cite This Antibody:
PhosphoSolutions Cat# p171-556, RRID:AB_2492092
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho Ser556 of human FANCI.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

Fanconi anemia, FA, is a rare disorder where cells cannot prevent, repair, or tolerate DNA damage, leading to cancer, progressive bone marrow failure and developmental abnormalities (Ishiai et al, 2008). 16 genes have been implicated in FA, and their products constitute a common FA pathway (Walden and Deans 2014). FANCI (Fanconi anemia complementation group I), is one of two substrates for monoubiquitination by the FANCL-containing core complex, and is crucial for DNA repair via FAP and intrastrand cross-links (Walden and Deans, 2014). Phosphorylation at Ser556 has been shown to play a key role in FANCI physically associating with FANCD2, stabilizing the ID2 complex, and supporting its DNA binding and subsequent D2-ubiquitination (Walden and Deans, 2014).

Synonyms: ExpandCollapse

• FANCI antibody
• FANCI gene antibody
• FANCI_HUMAN antibody
• Fanconi anemia group I protein antibody
• Fanconi anemia, complementation group I antibody
• FLJ10719 antibody
• FLJ14658 antibody
• KIAA1794 antibody
• Protein FACI antibody
• Protein FANCI antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific References

Cheung, R.S., Castella, M., Abeyta, A., Gafken, P.R., Tucker, N. and Taniguchi, T., 2017. Ubiquitination-Linked Phosphorylation of the FANCI S/TQ Cluster Contributes to Activation of the Fanconi Anemia I/D2 Complex. Cell Reports, 19(12), pp.2432-2440. PMID: 28636932

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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FANCI ser556 Antibody
Western blot of HeLa cell lysate showing specific immunolabeling of the ~150 kDa FANCI protein phosphorylated at Ser556 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (λ-Ptase, 800 units/1mg protein for 30 min).

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