Affinity purified from pooled serum. Learn more.

FAM129B (Ser679/683) Antibody

Catalog #: p170-679 Category: Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
100% Homology:
Mouse, Human, Non-human primate
Applications:
WB 1:1,000
Species:
Rabbit
Gene Name:
FAM129B
Molecular Reference:
~83 kDa
Cite This Antibody:
PhosphoSolutions Cat# p170-679, RRID:AB_2492091
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser 679/683 of the human FAM129B sequence.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

FAM129B, also known as Niban-like protein 1, belongs to a poorly characterized protein family with unknown category and function. Increased expression of the Niban gene has been observed in renal carcinomas (Adachi et al., 2004; Sun et al., 2007). Suppression of FAM129B expression in HeLa cells has been seen to promote apoptosis, suggesting that it can modulate cell death signaling, and may be involved in the ER stress response (Sun et al., 2007). FAM129B is also up-regulated in various types of thyroid tumors and Hashimoto’s thyroiditis (Matsumoto et al., 2006). It has been suggested that the MAP kinase dependent phosphorylation of FAM129B is important in controlling melanoma cells, as inhibition of B/Raf/MKK/ERK in melanoma cells represses invasion (Old et al., 2009). It is believed that phosphorylated FAM129B not only derepresses invasion, but also regulates events that promote invasion (Old et al., 2009).

Storage

Liquid in 100 mM Glycine, 200 mM Tris, pH 8.0. Contains no preservative.

For long term storage –20° C is recommended in undiluted aliquots. Stable at –20° C for at least 1 year. Avoid freeze/thaw cycles.

General References

Adachi H, Majima S, Kon S, Kobayashi T, Kajino K, Mitani H, Hirayama Y, Shiina H, Igawa M, Hino O. Niban gene is commonly expressed in the renal tumors: a new candidate marker for renal carcinogenesis. Oncogene. 2004 Apr22;23(19): 3495-500.

Sun GD, Kobayashi T, Abe M, Tada N, Adachi H, Siota A, Totsuka, Hino O. The endoplasmic reticulum stress-inducible protein Niban regulates eIF2alpha and S6K1/4E-BP1 phosphorylation. Biochem Biophys Res Commun. 2007 Aug 17;360(1):181-7.

Matsumoto F, Fujii H, Abe M, Kajino K, Kobayashi T, Matsumoto T, Ikeda K, Hino O. A novel tumor marker, Niban, is expressed in subsets of thyroid tumors and Hashimoto’s thyroiditis. Human Pathology. 2006 Dec;37(12):1592-600.

Old W, Shabb J, Houel S, Wang H, Couts K, Yen C, Litman E, Croy C, Meyer-Arendt K, Miranda J, Brown R, Witze E, Schweppe R, Resing K, Ahn N. Functional Proteomics Identifies Target of Phosphorylation by B-Raf Signaling in Melanoma. Molecular Cell. 2009 Apr 10(34): 115-131

FAM129B Ser679/683 Antibody
Western blot of 3T3 cell lysate showing specific immunolabeling of the ~83 kDa FAM129B protein phosphorylated at Ser679/683 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).

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