Affinity purified from pooled serum. Learn more.

FAM129B (Ser679/683) Antibody

We are the original manufacturer of our FAM129B (Ser679/683) rabbit polyclonal phosphospecific antibody, affinity purified from pooled serum. Optimized in WB.

Catalog #: p170-679 Categories: , Datasheet:
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$119.00 – $380.00

  • SizePrice
Clear
Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Human, Mouse
Expected Reactivity:
Non-human primate
Applications:
WB 1:1000 Don't see your application?
Host Species:
Rabbit Polyclonal
Gene Name:
FAM129B
Molecular Weight:
~83 kDa
Cite This Antibody:
PhosphoSolutions Cat# p170-679, RRID:AB_2492091
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser 679/683 of the human FAM129B sequence.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

FAM129B, also known as Niban-like protein 1, belongs to a poorly characterized protein family with unknown category and function. Increased expression of the Niban gene has been observed in renal carcinomas (Adachi et al., 2004; Sun et al., 2007). Suppression of FAM129B expression in HeLa cells has been seen to promote apoptosis, suggesting that it can modulate cell death signaling, and may be involved in the ER stress response (Sun et al., 2007). FAM129B is also up-regulated in various types of thyroid tumors and Hashimoto’s thyroiditis (Matsumoto et al., 2006). It has been suggested that the MAP kinase dependent phosphorylation of FAM129B is important in controlling melanoma cells, as inhibition of B/Raf/MKK/ERK in melanoma cells represses invasion (Old et al., 2009). It is believed that phosphorylated FAM129B not only derepresses invasion, but also regulates events that promote invasion (Old et al., 2009).

Synonyms: ExpandCollapse

• bA356B19.6 antibody
• C9orf88 antibody
• chromosome 9 open reading frame 88 antibody
• DKFZP434H0820 antibody
• FAM129B antibody
• family with sequence similarity 129, member B antibody
• FLJ13518 antibody
• FLJ22151 antibody
• FLJ22298 antibody
• hypothetical protein LOC64855 antibody
• Meg 3 antibody
• Meg-3 antibody
• Meg3 antibody
• Niban like protein 1 antibody
• Niban-like protein 1 antibody
• NIBL1_HUMAN antibody
• OC58 antibody
• OTTHUMP00000022187 antibody
• OTTHUMP00000022188 antibody
• Protein FAM129B antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

General References

Adachi, H., Majima, S., Kon, S., Kobayashi, T., Kajino, K., Mitani, H., Hirayama, Y., Shiina, H., Igawa, M. and Hino, O., 2004. Niban gene is commonly expressed in the renal tumors: a new candidate marker for renal carcinogenesis. Oncogene, 23(19), p.3495. PMID: 14990989

Sun, G.D., Kobayashi, T., Abe, M., Tada, N., Adachi, H., Shiota, A., Totsuka, Y. and Hino, O., 2007. The endoplasmic reticulum stress-inducible protein Niban regulates eIF2α and S6K1/4E-BP1 phosphorylation. Biochemical and Biophysical Research Communications, 360(1), pp.181-187. PMID: 17588536

Matsumoto, F., Fujii, H., Abe, M., Kajino, K., Kobayashi, T., Matsumoto, T., Ikeda, K. and Hino, O., 2006. A novel tumor marker, Niban, is expressed in subsets of thyroid tumors and Hashimoto’s thyroiditis. Human Pathology, 37(12), pp.1592-1600. PMID: 16949643

Old, W.M., Shabb, J.B., Houel, S., Wang, H., Couts, K.L., Yen, C.Y., Litman, E.S., Croy, C.H., Meyer-Arendt, K., Miranda, J.G. and Brown, R.A., 2009. Functional proteomics identifies targets of phosphorylation by B-Raf signaling in melanoma. Molecular Cell, 34(1), pp.115-131. PMID: 19362540

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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FAM129B Ser679/683 Antibody
Western blot of 3T3 cell lysate showing specific immunolabeling of the ~83 kDa FAM129B protein phosphorylated at Ser679/683 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).

Click here to view our Western blotting and lysate preparation protocols.

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