Affinity purified from pooled serum. Learn more.

DOPA Decarboxylase Antibody

$109.00$365.00

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Rabbit polyclonal antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Specificity:
Rat, Bovine
Applications:
WB 1:1,000
Species:
Rabbit
Gene Name:
DDC
Molecular Reference:
~55 kDa
Cite This Antibody:
PhosphoSolutions Cat# 417-DDC, RRID:AB_2492072
Antigen/Purification: ExpandCollapse

The antigen is an SDS denatured, recombinant bovine aromatic DOPA decarboxylase (DDC) expressed in E. coli and purified from inclusion bodies.

The antibody is prepared from pooled rabbit serum by affinity purification using an AminoLink® Plus column matrix to which purified, recombinant bovine DDC was coupled.

Biological Significance: ExpandCollapse

DOPA decarboxylase (aromatic L-amino acid decarboxylase, AADC; DDC) catalyzes the second reaction in the biosynthesis of catecholamines and serotonin (Waymire and Haycock, 2002; Berry et al., 1996; Haycock et al., 2003). It is also involved in the biosynthesis of trace amines. DDC antibodies can therefore be used as markers for dopaminergic, noradrenergic and serotonergic neurons in a variety of applications including depression, schizophrenia, Parkinson’s disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999).

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.

Product Specific References

Haycock JW, Becker L, Ang L, Furukawa Y, Hornykiewicz O, Kish SJ (2003) Marked disparity between age-related changes in dopamine and other presynaptic dopaminergic markers in human striatum. J Neurochem 87:574-585. PMID: 14535941

DOPA Decarboxylase Antibody
Western blot of rat adrenal medulla showing specific immunolabeling of the ~55 kDa DDC protein.

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