Affinity purified from pooled serum. Learn more.

DOPA Decarboxylase Antibody

We are the original manufacturer of our DOPA decarboxylase rabbit polyclonal total protein antibody, affinity purified from pooled serum. Optimized in WB.

Catalog #: 417-DDC Categories: , , , , Datasheet:

$119.00$380.00

  • SizePrice
Clear
Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Human, Mouse, Rat
Expected Reactivity:
Bovine
Applications:
WB 1:1000 Don't see your application?
Host Species:
Rabbit Polyclonal
Gene Name:
DDC
Molecular Weight:
~55 kDa
Cite This Antibody:
PhosphoSolutions Cat# 417-DDC, RRID:AB_2492072
Antigen/Purification: ExpandCollapse

The antigen is an SDS denatured, recombinant bovine aromatic DOPA decarboxylase (DDC) expressed in E. coli and purified from inclusion bodies.

The antibody is prepared from pooled rabbit serum by affinity purification using an AminoLink® Plus column matrix to which purified, recombinant bovine DDC was coupled.

Biological Significance: ExpandCollapse

DOPA decarboxylase (aromatic L-amino acid decarboxylase, AADC; DDC) catalyzes the second reaction in the biosynthesis of catecholamines and serotonin (Waymire and Haycock, 2002; Berry et al., 1996; Haycock et al., 2003). It is also involved in the biosynthesis of trace amines. DDC antibodies can therefore be used as markers for dopaminergic, noradrenergic and serotonergic neurons in a variety of applications including depression, schizophrenia, Parkinson’s disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999).

Synonyms: ExpandCollapse
  • AADC antibody
  • Aromatic L Amino Acid Decarboxylase antibody
  • Aromatic-L-amino-acid decarboxylase antibody
  • DDC antibody
  • DDC_HUMAN antibody
  • DOPA decarboxylase (aromatic L-amino acid decarboxylase) antibody
  • DOPA decarboxylase antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Western Blot: Human


Western Blot: Human
PMID Dilution Publication
14535941 1.2ug/ml Haycock, J.W., et al. 2003 Marked disparity between age‐related changes in dopamine and other presynaptic dopaminergic markers in human striatum. Journal of Neurochemistry, 87(3), pp.574-585.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
Leave a Reply

Your email address will not be published. Required fields are marked *

This site uses Akismet to reduce spam. Learn how your comment data is processed.

You may also like…