Affinity purified from pooled serum. Learn more.

DENND3 (Thr450) Antibody

Catalog #: p1030-450 Category: Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Specificity:
Human
Applications:
WB 1:1000
Species:
Rabbit Polyclonal
Gene Name:
DENND3
Molecular Reference:
~142 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1030-450, RRID:AB_2715755
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr450 of human DENND3.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

The DENN (differentially expressed in normal and neoplastic cells) domain (DENND) is a poorly characterized protein module conserved throughout evolution (McPherson et al., 2011). Proteins bearing a DENN domain have recently emerged as the largest family of Rab GEFs. Among these DENN domain proteins, DENND3 is a GEF for Rab12; promoting the exchange of GDP to GTP, converting inactive GDP-bound RAB12 into its active GTP-bound form (McPherson and Xu, 2015; Matsui et al., 2011).

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage –20°C is recommended. Stable at –20°C for at least 1 year.

General References

McPherson PS, Xu J (2015). DENND3: a signaling/trafficking interface in autophagy. Cell Cycle. 17: 2717-8.

Matsui T, Itoh T, Fukuda M (2011). Small GTPase Rab12 Regulates Constitutive Degradation of Transferrin Receptor. Traffic. 10: 1432-43.

McPherson PS, Dokainish H, Marat AL (2011). DENN Domain Proteins: Regulators of Rab GTPases. J Biol Chem. 16: 13791-800.

Western blot of HeLa cell lysate showing specific immunolabeling of the ~142 kDa DENND3 protein phosphorylated at Thr450 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (λ-Ptase, 800 units/1mg protein for 30 min).

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