Affinity purified from pooled serum. Learn more.

DENND3 (Thr450) Antibody

We are the original manufacturer of our DENND (Thr450) rabbit polyclonal phosphospecific antibody, affinity purified from pooled serum. Optimized in WB.

Catalog #: p1030-450 Categories: , Datasheet:

$119.00$380.00

  • SizePrice
Clear
Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Human
Applications:
WB 1:1000 Don't see your application?
Host Species:
Rabbit Polyclonal
Gene Name:
DENND3
Molecular Weight:
~142 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1030-450, RRID:AB_2715755
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr450 of human DENND3.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

The DENN (differentially expressed in normal and neoplastic cells) domain (DENND) is a poorly characterized protein module conserved throughout evolution (McPherson et al., 2011). Proteins bearing a DENN domain have recently emerged as the largest family of Rab GEFs. Among these DENN domain proteins, DENND3 is a GEF for Rab12; promoting the exchange of GDP to GTP, converting inactive GDP-bound RAB12 into its active GTP-bound form (McPherson and Xu, 2015; Matsui et al., 2011).

Synonyms: ExpandCollapse
  • DENN Domain Containing 3
  • DENN Domain-Containing Protein 3
  • DENN/MADD Domain Containing 3
  • KIAA0870 DENN/MADD domain containing 3
  • KIAA0870DENN domain-containing protein 3

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

General References

Xu, J. and McPherson, P.S., 2015. DENND3: a signaling/trafficking interface in autophagy. Cell Cycle, 14(17), p.2717. PMID: 26177209

Matsui, T., Itoh, T. and Fukuda, M., 2011. Small GTPase Rab12 regulates constitutive degradation of transferrin receptor. Traffic, 12(10), pp.1432-1443. PMID: 21718402

Marat, A.L., Dokainish, H. and McPherson, P.S., 2011. DENN domain proteins: regulators of Rab GTPases. Journal of Biological Chemistry, 286(16), pp.13791-13800. PMID: 21330364

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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