Affinity purified from pooled serum. Learn more.

DARPP 32 (Thr34) Antibody

Catalog #: p1025-34 Categories: , Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Mouse, Rat
Expected Reactivity:
Human, Bovine, Chicken, Non-human primate, Canine
Applications:
WB 1:1000 Don't see your application?
Species:
Rabbit
Gene Name:
PPP1R1B
Molecular Weight:
~32 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1025-34, RRID:AB_2492068
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr34 of rat DARPP-32.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

DARPP-32 is a dopamine (DA) and cAMP-regulated ~32k phosphoprotein that is associated with dopaminoceptive neurons (Fienberg et al., 1998). The protein inhibits protein phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA (Bibb et al., 1999). Phosphorylation of DARPP-32 is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac® (Maldve et al., 2002; Lindskog et al., 2002; Svenningsson et al., 2002).

Synonyms: ExpandCollapse
  • DARPP32 antibody
  • DARPP-32 antibody
  • Dopamine and cAMP regulated neuronal phosphoprotein 32 antibody
  • Dopamine and cAMP regulated neuronal phosphoprotein antibody
  • Dopamine and cAMP regulated phosphoprotein antibody
  • Dopamine and cAMP regulated phosphoprotein DARPP 32 antibody
  • Dopamine and cAMP regulated phosphoprotein DARPP32 antibody
  • Dopamine- and cAMP-regulated neuronal phosphoprotein antibody
  • FLJ20940 antibody
  • IPPD antibody
  • Neuronal phosphoprotein DARPP 32 antibody
  • PPP1R1B antibody
  • PPR1B_HUMAN antibody
  • Protein phosphatase 1 regulatory (inhibitor) subunit 1B antibody
  • Protein phosphatase 1 regulatory inhibitor subunit 1B antibody
  • Protein phosphatase 1 regulatory subunit 1B antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Western Blot: Mouse


Western Blot: Mouse
PMID Dilution Publication
26947946 not listed Bonito-Olivia, A., et al. 2016 Differential regulation of the phosphorylation of Trimethyl-lysine27 histone H3 at serine 28 in distinct populations of striatal projection neurons. Neuropharmacology, 107, pp.89-99.
26657176 not listed Bonito-Olivia, A., et al. 2016 Involvement of the striatal medium spiny neurons of the direct pathway in the motor stimulant effects of phencyclidine. International Journal of Neuropsychopharmacology, 19(6).
26459714 not listed Del’Guidice, T., et al. 2015 Selective disruption of dopamine D2-receptors/beta-arrestin2 signaling by mood stabilizers. Journal of Receptors and Signal Transduction, 1-9. Jun; 35(3):224-32.
26320830 not listed Beaulieu, J.M., et al. 2016 In vivo veritas, the next frontier for functionally selective GPCR ligands. Methods, 92, pp.64-71.
26240334 not listed Del’Guidice, T., et al. 2015 FXR1P is a GSK3β substrate regulating mood and emotion processing. Proceedings of the National Academy of Sciences, 112(33), pp.E4610-E4619.
21946266 not listed Errico, F., et al. 2011 Higher free D-aspartate and N-methyl-D-aspartate levels prevent striatal depotentiation and anticipate L-DOPA-induced dyskinesia. Experimental neurology, 232(2), pp.240-250.
21372109 not listed Marazziti, D., et al. 2011 Absence of the GPR37/PAEL receptor impairs striatal Akt and ERK2 phosphorylation, ΔFosB expression, and conditioned place preference to amphetamine and cocaine. The FASEB Journal, 25(6), pp.2071-2081.
20720111 1:1000 Napolitano, F., et al. 2010 Role of aberrant striatal dopamine D1 receptor/cAMP/protein kinase A/DARPP32 signaling in the paradoxical calming effect of amphetamine. Journal of Neuroscience, 30(33), pp.11043-11056.
19181855 not listed Maiya, R., et al. 2009 Tissue plasminogen activator modulates the cellular and behavioral response to cocaine. Proceedings of the National Academy of Sciences, 106(6), pp.1983-1988.
18234897 1:1000 Niculescu, M., et al. 2008 Trk: a neuromodulator of age-specific behavioral and neurochemical responses to cocaine in mice. Journal of Neuroscience, 28(5), pp.1198-1207.
16954211 not listed Beaulieu, J.M., et al. 2006 Paradoxical striatal cellular signaling responses to psychostimulants in hyperactive mice. Journal of Biological Chemistry, 281(43), pp.32072-32080.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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DARPP 32 thr34 Antibody
Western blot of rat striatal lysate showing specific immunolabeling of the ~32 kDa DARPP-32 phosphorylated at Thr34 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).

Click here to view our Western blotting and lysate preparation protocols.

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