Affinity purified from pooled serum. Learn more.

DARPP 32 (Thr34) Antibody

Catalog #: p1025-34 Category: Datasheet:

$109.00$365.00

  • SizePrice
Clear

Rabbit polyclonal antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Reactivity:
Rat, Mouse, Human, Bovine, Chicken, Non-human primate, Canine
Applications:
WB 1:1000
Species:
Rabbit
Gene Name:
PPP1R1B
Molecular Reference:
~32 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1025-34, RRID:AB_2492068
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr34 of rat DARPP-32.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

DARPP-32 is a dopamine (DA) and cAMP-regulated ~32k phosphoprotein that is associated with dopaminoceptive neurons (Fienberg et al., 1998). The protein inhibits protein phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA (Bibb et al., 1999). Phosphorylation of DARPP-32 is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac® (Maldve et al., 2002; Lindskog et al., 2002; Svenningsson et al., 2002).

Synonyms: ExpandCollapse
  • DARPP32 antibody
  • DARPP-32 antibody
  • Dopamine and cAMP regulated neuronal phosphoprotein 32 antibody
  • Dopamine and cAMP regulated neuronal phosphoprotein antibody
  • Dopamine and cAMP regulated phosphoprotein antibody
  • Dopamine and cAMP regulated phosphoprotein DARPP 32 antibody
  • Dopamine and cAMP regulated phosphoprotein DARPP32 antibody
  • Dopamine- and cAMP-regulated neuronal phosphoprotein antibody
  • FLJ20940 antibody
  • IPPD antibody
  • Neuronal phosphoprotein DARPP 32 antibody
  • PPP1R1B antibody
  • PPR1B_HUMAN antibody
  • Protein phosphatase 1 regulatory (inhibitor) subunit 1B antibody
  • Protein phosphatase 1 regulatory inhibitor subunit 1B antibody
  • Protein phosphatase 1 regulatory subunit 1B antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.

Product Specific References

Bonito-Oliva, A., Södersten, E., Spigolon, G., Hu, X., Hellysaz, A., Falconi, A., Gomes, A.L., Broberger, C., Hansen, K., & Fisone, G. (2016). Differential regulation of the phosphorylation of Trimethyl-lysine27 histone H3 at serine 28 in distinct populations of striatal projection neurons. Neuropharmacology, 107, 89-99.  PMID: 26947946

Beaulieu, J. M. (2015). In vivo veritas, the next frontier for functionally selective GPCR ligands. Methods. Aug 28. PMID: 26320830

Bonito-Oliva, A., DuPont, C., Madjid, N., Ögren, S. O., & Fisone, G. (2015). Involvement of the striatal medium spiny neurons of the direct pathway in the motor stimulant effects of phencyclidine. The international journal of neuropsychopharmacology/(CINP). PMID: 26657176

Del’Guidice, T., & Beaulieu, J. M. (2015). Selective disruption of dopamine D2-receptors/beta-arrestin2 signaling by mood stabilizers. Journal of Receptors and Signal Transduction, 1-9. Jun;35(3):224-32. PMID: 26459714

Thomas Del’Guidice, Camille Latapy, Antonio Rampino, Jivan Khlghatyan, Morgane Lemasson, Barbara Gelao, Tiziana Quarto, Giuseppe Rizzo, Annie Barbeau, Claude Lamarre, Alessandro Bertolino, Giuseppe Blasi, and Jean-Martin Beaulieu (2015) FXR1P is a GSK3β substrate regulating mood and emotion processing. PNAS, Aug 18;112(33):E4610-9. PMID: 26240334

Errico, F., Bonito-Oliva, A., Bagetta, V., Vitucci, D., Romano, R., Zianni, E., Napolitano, F., Marinucci, S., Di Luca, M., Calabresi, P. and Fisone, G., 2011. Higher free D-aspartate and N-methyl-D-aspartate levels prevent striatal depotentiation and anticipate L-DOPA-induced dyskinesia. Experimental neurology232(2), pp.240-250. PMID: 21946266

Daniela Marazziti, Chiara Di Pietro, Silvia Mandillo, Elisabetta Golini, Rafaele Matteoni, and Glauco P. Tocchini-Valentini (2011) Absence of the GPR37/PAEL receptor impairs striatal Akt and ERK2 phosphorylation, FosB expression, and conditioned place preference to amphetamine and cocaine. FASEB J, 25: 2071 – 2081. PMID: 21372109

Napolitano, F., Bonito-Oliva, A., Federici, M., Carta, M., Errico, F., Magara, S., Martella, G., Nistico, R., Centonze, D., Pisani, A. and Gu, H.H., 2010. Role of aberrant striatal dopamine D1 receptor/cAMP/protein kinase A/DARPP32 signaling in the paradoxical calming effect of amphetamine. Journal of Neuroscience, 30(33), pp.11043-11056. PMID: 20720111

Rajani Maiya, Yan Zhou, Erin H. Norris, Mary Jeanne Kreek, and Sidney Strickland (2009) Tissue plasminogen activator modulates the cellular and behavioral response to cocaine. PNAS, 106: 1983 – 1988. PMID: 19181855

Michelle Niculescu, Shane A. Perrine, Jonathan S. Miller, Michelle E. Ehrlich, and Ellen M. Unterwald (2008) Trk: A Neuromodulator of Age-Specific Behavioral and Neurochemical Responses to Cocaine in Mice. J. Neurosci., Jan 2008; 28: 1198 – 1207. PMID: 18234897

Jean-Martin Beaulieu, Tatyana D. Sotnikova, Raul R. Gainetdinov, and Marc G. Caron (2006) Paradoxical Striatal Cellular Signaling Responses to Psychostimulants in Hyperactive Mice. J. Biol. Chem., Oct 2006; 281: 32072 – 32080. PMID: 16954211

Reviews

There are no reviews yet.

Be the first to review “DARPP 32 (Thr34) Antibody”

Your email address will not be published. Required fields are marked *

This site uses Akismet to reduce spam. Learn how your comment data is processed.

DARPP 32 thr34 Antibody
Western blot of rat striatal lysate showing specific immunolabeling of the ~32 kDa DARPP-32 phosphorylated at Thr34 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).

You may also like…