Affinity purified from pooled serum. Learn more.

CREB (Ser133) Antibody

Catalog #: p1010-133 Categories: , Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Mouse, Rat
Expected Reactivity:
Human, Xenopus, Bovine, Chicken, Non-human primate, Zebra fish, Sheep, Canine
Applications:
WB 1:1000IHC 1:100-1:1000
Species:
Rabbit
Gene Name:
CREB1
Molecular Weight:
~45 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1010-133, RRID:AB_2492066
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser133 of rat CREB.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

It is well known that the control of gene expression involves activation of protein kinase cascades that regulate transcription factors within the nucleus (Karin and Hunter, 1995). The cyclic AMP response element binding protein (CREB) is one of the best characterized stimulus-induced transcription factors (Montminy, 1997). This transcription factor is a component of intracellular signaling events that regulate a wide range of biological functions, from spermatogenesis to circadian rhythms and memory (Shaywitz and Greenberg, 1999; Silva et al., 1998). A variety of protein kinases including protein kinase A (PKA), mitogen-activated protein kinases (MAPKs), and Ca2+/calmodulin-dependent protein kinases (CaMKs) phosphorylate CREB at serine 133 (Ser133), and phosphorylation of Ser133 are required for CREB-mediated transcription (Johannessen et al., 2004; Kornhauser et al., 2002).

Synonyms: ExpandCollapse
  • Active transcription factor CREB antibody
  • cAMP response element binding protein 1 antibody
  • cAMP response element binding protein antibody
  • cAMP responsive element binding protein 1 antibody
  • cAMP-responsive element-binding protein 1 antibody
  • CREB antibody
  • CREB-1 antibody
  • CREB1 antibody
  • CREB1_HUMAN antibody
  • Cyclic AMP-responsive element-binding protein 1 antibody
  • MGC9284 antibody
  • OTTHUMP00000163864 antibody
  • OTTHUMP00000163865 antibody
  • OTTHUMP00000206660 antibody
  • OTTHUMP00000206662 antibody
  • OTTHUMP00000206667 antibody
  • Transactivator protein antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Immunohistochemistry: Mouse
Western Blotting: Mouse


Immunohistochemistry: Mouse
PMID Dilution Publication
27766068 not listed Bu, W., et al. 2016. Mild traumatic brain injury produces neuron loss that can be rescued by modulating microglial activation using a CB2 receptor inverse agonist. Frontiers in neuroscience, 10, p.449.


Western Blotting: Mouse
PMID Dilution Publication
31216095 1:500 Abdurakhmanova, S., et al. 2019. Abnormal behavior, striatal dopamine turnover and opioid peptide gene expression in histamine‐deficient mice. Genes, Brain and Behavior, p.e12595.
29367010 not listed Yau, S.Y., et al. 2018. Chronic minocycline treatment improves hippocampal neuronal structure, NMDA receptor function, and memory processing in Fmr1 knockout mice. Neurobiology of disease, 113, pp.11-22.
28650315 1:300 Du, J., et al. 2016. Transient acidosis while retrieving a fear-related memory enhances its lability. eLife, 6, p.e22564.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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CREB ser133 Antibody
Western blot of rat hippocampal lysate stimulated with forskolin showing specific immunolabeling of the ~45 kDa CREB phosphorylated at Ser133 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (λ-Ptase, 800 units/1mg protein for 30 min).

Click here to view our Western blotting and lysate preparation protocols.

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