Affinity purified from pooled serum. Learn more.

CaM Kinase II (Thr286) Antibody

Catalog #: p1005-286 Category: Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Tested:
Human, Mouse, Rabbit, Rat
Species Reactivity:
Zebrafish
Applications:
WB 1:1000IHC 1:1000ICC (Samikkannu, T. et al, 2015) Don't see your application?
Species:
Rabbit
Gene Name:
CAMK2A/B
Molecular Weight:
~50 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1005-286, RRID:AB_2492051
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr286 found in rat brain CaM Kinase II.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

 

Biological Significance: ExpandCollapse

Ca2+/calmodulin-dependent protein kinase II (CaM Kinase II) is a multi-functional calcium and calmodulin-dependent protein kinase that mediates cellular responses to a wide variety of intercellular signals (Kennedy, 1998; Schulman and Hanson, 1993). CaM Kinase II has been shown to regulate diverse cellular functions including synaptic plasticity, neurotransmitter synthesis and release, gene expression, ion channel function, carbohydrate metabolism, cytoskeletal function, and Ca2+-homeostasis (Gleason et al., 2003; Soderling, 2000; Hudmon and Schulman, 2002). Phosphorylation of Thr286 on the kinase produces an autonomously active form of CaM Kinase II (Meng et al., 2003; Picciotto et al., 1993). Autophosphorylation of Thr305 inhibits the activity CaM Kinase II. Phosphorylation at this site appears to reduce the association of CaM Kinase II with the PSD and reduce LTP and learning (Elgersma et al., 2002).

Synonyms: ExpandCollapse
  • Calcium/calmodulin dependent protein kinase II alpha antibody
  • Calcium/calmodulin dependent protein kinase II beta antibody
  • Calcium/calmodulin dependent protein kinase II delta antibody
  • Calcium/calmodulin dependent protein kinase II gamma antibody
  • Calcium/calmodulin-dependent protein kinase type II subunit alpha antibody
  • CaM kinase II alpha antibody
  • CaM kinase II antibody
  • CaM kinase II beta antibody
  • CaM kinase II delta antibody
  • CaM kinase II gamma antibody
  • CaM kinase II subunit alpha antibody
  • CaMK-II subunit alpha antibody
  • CAMK2 antibody
  • Camk2a antibody
  • CAMK2B antibody
  • CAMK2D antibody
  • CAMK2G antibody
  • CAMKA antibody
  • KCC2A_HUMAN antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Product Specific Protocols

Western Blotting

Click here to view our protocols page for Western blotting and lysate preparation.

Product Specific References for Applications and Species

Immunocytochemistry: Human
Immunohistochemistry: Rabbit
Western Blot: Human | Mouse | Rat


Immunocytochemistry: Human
PMID Dilution Publication
26057350 not listed Samikkannu, T., et al. 2015 HIV subtypes B and C gp120 and methamphetamine interaction: dopaminergic system implicates differential neuronal toxicity. Scientific reports, 5, p.11130.


Immunohistochemistry: Rabbit
PMID Dilution Publication
22593045 1:1000 Kothmann, W.W., et al. 2012 Nonsynaptic NMDA Receptors Mediate Activity-Dependent Plasticity of Gap Junctional Coupling in the All Amacrine Cell Network. J. Neurosci. 32(3): 6747-6759.


Western Blot: Human
PMID Dilution Publication
30143647 1:1000 Lee, S.H., et al. 2018 Reciprocal control of excitatory synapse numbers by Wnt and Wnt inhibitor PRR7 secreted on exosomes. Nature communications, 9(1), p.3434.
28318784 not listed Murakoshi, H., et al. 2017 Kinetics of Endogenous CaMKII Required for Synaptic Plasticity Revealed by Optogenetic Kinase Inhibitor. Neuron. Apr 5;94(1):37-47.e5.
26057350 not listed Samikkannu, T., et al. 2015 HIV subtypes B and C gp120 and methamphetamine interaction: dopaminergic system implicates differential neuronal toxicity. Scientific reports, 5, p.11130.
24855644 1:3000 Coultrap, S.J., et al. 2014 Nitric oxide induces Ca2+-independent activity of the Ca2+/calmodulin-dependent protein kinase II (CaMKII). J. Biol Chem. 289(28):19458-65.
23255606 1:10,000 Magupalli, V.G., et al. 2013 Ca2+-independent activation of Ca2+/calmodulin-dependent protein kinase II bound to the C-terminal domain of CaV2. 1 calcium channels. Journal of Biological Chemistry, 288(7), 4637-4648.


Western Blot: Mouse
PMID Dilution Publication
30282026 1:1000 Glasgow, S.D., et al. 2018 Activity-dependent netrin-1 secretion drives synaptic insertion of GluA1-containing AMPA receptors in the hippocampus. Cell Rep, 25(1):168-182.
27622724 not listed Huang, L., et al. 2017 Long-lasting behavioral effects in neonatal mice with multiple exposures to ketamine-xylazine anesthesia. Neurotoxicology and Teratology.
18417701 1:10,000 Sun, X., et al. 2008 Acute and Chronic Dopamine Receptor Stimulation Modulates AMPA Receptor Trafficking in Nucleus Accumbens Neurons Cocultured with Prefrontal Cortex Neurons. J. Neurosci., 28: 4216 – 4230.
17855343 not lited Townsend, M., et al. 2008 Soluble A-beta Inhibits Specific Signal Transduction Cascades Common to the Insulin Receptor Pathway. J. Biol. Chem., 282: 33305 – 33312.


Western Blot: Rat
PMID Dilution Publication
30143647 1:1000 Lee, S.H., et al. 2018 Reciprocal control of excitatory synapse numbers by Wnt and Wnt inhibitor PRR7 secreted on exosomes. Nature communications, 9(1), p.3434.
28318784 not listed Murakoshi, H., et al. 2017 Kinetics of Endogenous CaMKII Required for Synaptic Plasticity Revealed by Optogenetic Kinase Inhibitor. Neuron. Apr 5;94(1):37-47.e5.
24843070 1:3000 Barcomb, K., et al. 2014 Autonomous CaMKII requires further stimulation by Ca2+/calmodulin for enhancing synaptic strength. The FASEB Journal, 28(8), 3810-3819.
24780380 1:2000 Bernard, P.B., et al. 2014 Necessary, but not sufficient: insights into the mechanisms of mGluR mediated long-term depression from a rat model of early life seizures. Neuropharmacology, 84, 1-12.
23143515 1:1000 Shin, S.M., et al. 2012 GKAP orchestrates activity-dependent postsynaptic protein remodeling and homeostatic scaling. Nature neuroscience, 15(12), p.1655-1666.
20424167 not listed Vest, R.S., et al. 2010 Dual Mechanism of a Natural CaMKII Inhibitor. Mol. Biol. Cell, 18: 5024-5033.
20353941 not listed Coultrap, S.J., et al. 2010 CaMKII Autonomy Is Substrate-dependent and Further Stimulated by Ca2+/Calmodulin. J. Biol. Chem., 285: 17930 – 17937.
18819923 1:1000 Davies, K.D., et al. 2008 Long Term Synaptic Depression That Is Associated with GluR1 Dephosphorylation but Not Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid (AMPA) Receptor Internalization. J. Biol. Chem., 283: 33138 – 33146.
18417701 1:10,000 Sun, X., et al. 2008 Acute and Chronic Dopamine Receptor Stimulation Modulates AMPA Receptor Trafficking in Nucleus Accumbens Neurons Cocultured with Prefrontal Cortex Neurons. J. Neurosci., 28: 4216 – 4230.
17942605 1:500 Sun, X., et al. 2008 Dual Mechanism of a Natural CaMKII Inhibitor. Mol. Biol. Cell, 18: 5024 – 5033.

  • 5 – Excellent (publishable, performed ideally)
  • 4 – Good (publishable, would use again)
  • 3 – Average (publishable, might use again)
  • 2 – Poor (unpublishable, signal inconclusive)
  • 1 – No signal (unpublishable)
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CaM Kinase II thr286 Antibody
Western blot of rat brain lysate showing specific immunolabeling of the ~50 kDa α- and the ~60 kDa β-CaM Kinase II phosphorylated at Thr286 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).

Click here to view our Western blotting and lysate preparation protocols.

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