Affinity purified from pooled serum. Learn more.

CaM Kinase II (Thr286) Antibody

Catalog #: p1005-286 Category: Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Reactivity:
Mouse, Rat, Human, Zenopus
Applications:
WB 1:1000IF 1:1000 (Kothmann et al. 2012)
Species:
Rabbit
Gene Name:
CAMK2A/B
Molecular Reference:
~50 kDa
Cite This Antibody:
PhosphoSolutions Cat# p1005-286, RRID:AB_2492051
Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr286 found in rat brain CaM Kinase II.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

 

Biological Significance: ExpandCollapse

Ca2+/calmodulin-dependent protein kinase II (CaM Kinase II) is a multi-functional calcium and calmodulin-dependent protein kinase that mediates cellular responses to a wide variety of intercellular signals (Kennedy, 1998; Schulman and Hanson, 1993). CaM Kinase II has been shown to regulate diverse cellular functions including synaptic plasticity, neurotransmitter synthesis and release, gene expression, ion channel function, carbohydrate metabolism, cytoskeletal function, and Ca2+-homeostasis (Gleason et al., 2003; Soderling, 2000; Hudmon and Schulman, 2002). Phosphorylation of Thr286 on the kinase produces an autonomously active form of CaM Kinase II (Meng et al., 2003; Picciotto et al., 1993). Autophosphorylation of Thr305 inhibits the activity CaM Kinase II. Phosphorylation at this site appears to reduce the association of CaM Kinase II with the PSD and reduce LTP and learning (Elgersma et al., 2002).

Synonyms: ExpandCollapse
  • Calcium/calmodulin dependent protein kinase II alpha antibody
  • Calcium/calmodulin dependent protein kinase II beta antibody
  • Calcium/calmodulin dependent protein kinase II delta antibody
  • Calcium/calmodulin dependent protein kinase II gamma antibody
  • Calcium/calmodulin-dependent protein kinase type II subunit alpha antibody
  • CaM kinase II alpha antibody
  • CaM kinase II antibody
  • CaM kinase II beta antibody
  • CaM kinase II delta antibody
  • CaM kinase II gamma antibody
  • CaM kinase II subunit alpha antibody
  • CaMK-II subunit alpha antibody
  • CAMK2 antibody
  • Camk2a antibody
  • CAMK2B antibody
  • CAMK2D antibody
  • CAMK2G antibody
  • CAMKA antibody
  • KCC2A_HUMAN antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.

Product Specific References

Glasgow, S.D., Labrecque, S., Beamish, I.V., Aufmkolk, S., Gibon, J., Han, D., Harris, S.N., Dufresne, P., Wiseman, P.W., McKinney, R.A. and Seguela, P., 2018. Activity-dependent netrin-1 secretion drives synaptic insertion of GluA1-containing AMPA receptors in the hippocampus. Cell Rep, 25(1):168-182. PMID: 30282026

Lee, S.H., Shin, S.M., Zhong, P., Kim, H.T., Kim, D.I., Kim, J.M., Do Heo, W., Kim, D.W., Yeo, C.Y., Kim, C.H. and Liu, Q.S., 2018. Reciprocal control of excitatory synapse numbers by Wnt and Wnt inhibitor PRR7 secreted on exosomes. Nature communications, 9(1), p.3434. PMID: 30143647

Murakoshi, H., Shin, M.E., Parra-Bueno, P., Szatmari, E.M., Shibata, A.C. and Yasuda, R., 2017. Kinetics of Endogenous CaMKII Required for Synaptic Plasticity Revealed by Optogenetic Kinase Inhibitor. Neuron. Apr 5;94(1):37-47.e5. PMID: 28318784

Huang, L., Hayes, S. and Yang, G., 2016. Long-lasting behavioral effects in neonatal mice with multiple exposures to …-xylazine anesthesia. Neurotoxicology and Teratology. PMID: 27622724

Samikkannu, T., Rao, K.V., Salam, A.A.A., Atluri, V.S., Kaftanovskaya, E.M., Agudelo, M., Perez, S., Yoo, C., Raymond, A.D., Ding, H. and Nair, M.P., 2015. HIV subtypes B and C gp120 and methamphetamine interaction: dopaminergic system implicates differential neuronal toxicity. Scientific reports, 5, p.11130. PMID: 26057350

Barcomb, K., Buard, I., Coultrap, S. J., Kulbe, J. R., O’Leary, H., Benke, T. A., & Bayer, K. U. (2014). Autonomous CaMKII requires further stimulation by Ca2+/calmodulin for enhancing synaptic strength. The FASEB Journal, 28(8), 3810-3819. PMID: 24843070

Bernard, P. B., Castano, A. M., Bayer, K. U., & Benke, T. A. (2014). Necessary, but not sufficient: insights into the mechanisms of mGluR mediated long-term depression from a rat model of early life seizures. Neuropharmacology, 84, 1-12. PMID: 24780380

Steven J. Coultrap, K. Ulrich Bayer (2014) Nitric oxide induces Ca2+-independent activity of the Ca2+/calmodulin-dependent protein kinase II (CaMKII). J. Biol Chem. 289(28):19458-65. PMID: 24855644

Magupalli, V. G., Mochida, S., Yan, J., Jiang, X., Westenbroek, R. E., Nairn, A. C., Scheuer, T., & Catterall, W. A. (2013). Ca2+-independent activation of Ca2+/calmodulin-dependent protein kinase II bound to the C-terminal domain of CaV2. 1 calcium channels. Journal of Biological Chemistry, 288(7), 4637-4648. PMID: 23255606

Shin, S.M., Zhang, N., Hansen, J., Gerges, N.Z., Pak, D.T., Sheng, M. and Lee, S.H., 2012. GKAP orchestrates activity-dependent postsynaptic protein remodeling and homeostatic scaling. Nature neuroscience, 15(12), p.1655-1666. PMID: 23143515

W. Wade Kothmann, E. Brady Trexler, Christopher M. Whitaker, Wei Li, Stephen C. Massey, and John O’Brien (2012) Nonsynaptic NMDA Receptors Mediate Activity-Dependent Plasticity of Gap Junctional Coupling in the All Amacrine Cell Network. J. Neurosci. 32(3): 6747-6759. PMID: 22593045

Rebekah S. Vest, Heather O’Leary, Steven J. Coultrap, Mark S. Kindy, and K. Ulrich Bayer (2010) Effective Post-insult Neuroprotection by a Novel Ca2+/ Calmodulin-dependent Protein Kinase II (CaMKII) InhibitorJ. Biol. Chem., 285: 20675 – 20682. PMID: 20424167

Steven J. Coultrap, Isabelle Buard, Jaqueline R. Kulbe, Mark L. Dell’Acqua, and K. Ulrich Bayer (2010) CaMKII Autonomy Is Substrate-dependent and Further Stimulated by Ca2+/Calmodulin J. Biol. Chem., 285: 17930 – 17937. PMID: 20353941

Kurtis D. Davies, Susan M. Goebel-Goody, Steven J. Coultrap, and Michael D. Browning (2008) Long Term Synaptic Depression That Is Associated with GluR1 Dephosphorylation but Not Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid (AMPA) Receptor Internalization J. Biol. Chem., 283: 33138 – 33146. PMID: 18819923

Xiu Sun, Michael Milovanovic, Yun Zhao, and Marina E. Wolf (2008) Acute and Chronic Dopamine Receptor Stimulation Modulates AMPA Receptor Trafficking in Nucleus Accumbens Neurons Cocultured with Prefrontal Cortex Neurons. J. Neurosci., 28: 4216 – 4230. PMID: 18417701

Matthew Townsend, Tapan Mehta, and Dennis J. Selkoe (2007) Soluble A-beta Inhibits Specific Signal Transduction Cascades Common to the Insulin Receptor Pathway. J. Biol. Chem., 282: 33305 – 33312. PMID: 17855343

Rebekah S. Vest, Kurtis D. Davies, Heather O’Leary, J. David Port, and K. Ulrich Bayer (2007) Dual Mechanism of a Natural CaMKII Inhibitor. Mol. Biol. Cell, 18: 5024 – 5033. PMID: 17942605

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CaM Kinase II thr286 Antibody
Western blot of rat brain lysate showing specific immunolabeling of the ~50 kDa α- and the ~60 kDa β-CaM Kinase II phosphorylated at Thr286 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).

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