Affinity purified from pooled serum. Learn more.

Androgen Receptor (AR) (Ser94) Antibody

Catalog #: p111-94 Category: Datasheet:

$109.00$365.00

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Rabbit polyclonal antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Species Reactivity:
Human, Non-human primate
Applications:
WB 1:1000
Species:
Rabbit
Gene Name:
AR
Molecular Reference:
~110 kDa
Cite This Antibody:
PhosphoSolutions Cat# p111-94, RRID:AB_2492040
Antigen/Purification: ExpandCollapse

The antigen is a synthetic phospho-peptide surrounding the phospho Ser94 of human AR.

The antibody is  prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns

Biological Significance: ExpandCollapse

The androgen receptor (AR) is a DNA-binding transcription factor that regulates genes critical for the development and maintenance of the male sexual phenotype. Defects in androgen receptor have been shown to play a role in prostate cancer, and inhibition of AR activity through modulation of signal transduction pathways may delay prostate cancer progression (Heinlein and Chang 2004). Multiple phosphorylation sites have been identified on the androgen receptor that affect cross-talk between growth factor signaling and androgen in prostate development and cancer (Gioeli et al., 2002). One of these sites, at Ser94, appears constitutively phosphorylated and exhibits no response to treatments with stimulating hormone (Gioeli et al., 2002). The site at Ser94 is unique among the AR phosphorylation sites in that it does not achieve a maximal level of phosphorylation between translation and the initial round of nuclear import, having a strong bias for androgen-independent phosphorylation in the cytoplasm (Kesler et al., 2007).

Synonyms: ExpandCollapse
  • AIS antibody
  • ANDR_HUMAN antibody
  • Androgen nuclear receptor variant 2 antibody
  • Androgen receptor (dihydrotestosterone receptor; testicular feminization; spinal and bulbar muscular atrophy; Kennedy disease) antibody
  • Androgen receptor antibody
  • androgen receptor splice variant 4b antibody
  • AR antibody
  • AR8 antibody
  • DHTR antibody
  • Dihydro testosterone receptor antibody
  • Dihydrotestosterone receptor (DHTR) antibody
  • Dihydrotestosterone receptor antibody
  • HUMARA antibody
  • HYSP1 antibody
  • KD antibody
  • Kennedy disease (KD) antibody
  • NR3C4 antibody
  • Nuclear receptor subfamily 3 group C member 4 (NR3C4) antibody
  • Nuclear receptor subfamily 3 group C member 4 antibody
  • SBMA antibody
  • SMAX1 antibody
  • Spinal and bulbar muscular atrophy (SBMA) antibody
  • Spinal and bulbar muscular atrophy antibody
  • Testicular Feminization (TFM) antibody
  • TFM antibody

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.

General References

Heinlein C, Chang C. (2004) Androgen Receptor in Prostate Cancer. Endocrine Reviews. 25(2):276-308

Gioeli D, Ficarro S, Kwiek J, Aaronson D, Hancock M, Catling A, White F, Christian R, Settlage R, Shabanowitz J, Hunt D, Weber M. (2002) Androgen Receptor Phosphorylation, Regulation and Indentification of the Phosphorylation Sites. J Biol Chem. 277(32):29304-29314

Kesler C, Gioeli D, Conaway M, Weber M, Paschal B. (2007) Subcellular Localization Modulates Activation Function 1 Domain Phosphorylation in the Androgen Receptor. Molecular Endocrinology. 21(9):2071-2084

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Androgen Receptor (AR) ser94 Antibody
Western blot of HeLa lysate showing specific immunolabeling of the ~110 kDa AR protein phosphorylated at Ser94 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding non-phosphopeptide (not shown).

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