Affinity purified from pooled serum. Learn more.

14-3-3 Protein Antibody

Catalog #: 1433-P Category: Datasheet:

$109.00$365.00

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Rabbit Polyclonal Antibody

Pooled Serum
Formulation:
Affinity Purified from Pooled Serum
Specificity:
Rat, Mouse, Human, Xenopus, Bovine, Chicken, Non-human primate, Zebra fish, Canine
Applications:
WB 1:1000IHC Not recommended for use in paraffin sections
Species:
Rabbit
Gene Name:
YWHAB
Molecular Reference:
~29 kDa
Cite This Antibody:
PhosphoSolutions Cat# 1433-P, RRID:AB_2492026
Antigen/Purification: ExpandCollapse

The antigen is a peptide corresponding to amino acid residues from the N-terminal region of the rat 14-3-3 protein, conjugated to keyhole limpet hemocyanin (KLH).

The antibody is prepared from pooled rabbit serum by affinity purification via chromatography on an affinity column prepared with the N-terminal peptide used as antigen.

Biological Significance: ExpandCollapse

14-3-3 proteins are a family of highly conserved proteins that appear to have multiple roles in cell signaling (Bridges and Moorhead, 2005). The proteins are abundantly expressed in the brain and have been detected in the cerebrospinal fluid of patients with different neurological disorders (Berg et al., 2003). 14-3-3 proteins bind protein ligands that are typically phosphorylated on serine or threonine residues and regulate the functions of these binding partners by a number of different mechanisms (Silhan et al., 2004; Dougherty and Morrison, 2004). The 14-3-3 proteins affect a diverse array of cellular processes including the cell cycle and transcription, signal transduction and intracellular trafficking.

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.

Product Specific References

Irina Surgucheva, Valery I. Shestopalov, and Andrei Surguchov (2008) Effect of gamma-Synuclein Silencing on Apoptotic Pathways in Retinal Ganglion Cells J. Biol. Chem., 283: 36377 – 36385. PMID: 18936092

14-3-3 Protein Antibody
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~29 kDa 14-3-3 protein.

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