Rabbit Polyclonal AntibodyPooled Serum
- Affinity Purified from Pooled Serum
- Rat, Mouse, Human, Xenopus, Bovine, Chicken, Non-human primate, Zebra fish, Canine
- WB 1:1000IHC Not recommended for use in paraffin sections
- Gene Name:
- Molecular Reference:
- ~29 kDa
- Cite This Antibody:
- PhosphoSolutions Cat# 1433-P, RRID:AB_2492026
The antigen is a peptide corresponding to amino acid residues from the N-terminal region of the rat 14-3-3 protein, conjugated to keyhole limpet hemocyanin (KLH).
The antibody is prepared from pooled rabbit serum by affinity purification via chromatography on an affinity column prepared with the N-terminal peptide used as antigen.
Biological Significance: ExpandCollapse
14-3-3 proteins are a family of highly conserved proteins that appear to have multiple roles in cell signaling (Bridges and Moorhead, 2005). The proteins are abundantly expressed in the brain and have been detected in the cerebrospinal fluid of patients with different neurological disorders (Berg et al., 2003). 14-3-3 proteins bind protein ligands that are typically phosphorylated on serine or threonine residues and regulate the functions of these binding partners by a number of different mechanisms (Silhan et al., 2004; Dougherty and Morrison, 2004). The 14-3-3 proteins affect a diverse array of cellular processes including the cell cycle and transcription, signal transduction and intracellular trafficking.