Our Anti-Tubulin, beta III primary antibody from PhosphoSolutions is mouse monoclonal. It detects mouse and rat Tubulin, beta III and is Protein G purified. It is great for use in WB, IHC, ICC.
Immunostaining of mouse dentate gyrus showing beta III tubulin (red, 1:1000). The blue is staining nuclei. Photo courtesy of Robert Wine.
Tubulin is the major constituent of microtubules, existing as a heterodimer of the α and β subunits. The beta III isoform of tubulin is found almost exclusively in neuronal processes of adult tissues and is therefore an excellent marker for neurons. Neuron specific, posttranslational modifications within the C-terminal domain of beta III tubulin have been shown to be developmentally regulated suggesting that they may serve to modulate the interaction of tubulin with microtubule associated proteins (Lee et al., 1990). Additionally, beta III tubulin has been found to be highly expressed in cancer cells such as small cell lung cancer, large cell neuroendocrine carcinoma and adenocarcinomas and is correlated with an increasing histological degree of malignancy (Katsetos et al., 2003)
Protein G Purified
Monoclonal
AA10
IgG
IHC, WB
Mouse
TUBB3
55 kDa
Synthetic peptide corresponding to amino acid residues specific to rat beta III tubulin, conjugated to keyhole limpet hemocyanin (KLH).
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Protein G purified culture supernatant.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:10000
WB Brain: 1:10000
IHC: 1:1000
Unconjugated
Specific for endogenous levels of the ~55 kDa beta III tubulin protein. This clone is similar to the monoclonal antibody Tuj1.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Cooper, R., et al. 2015. Silver nanoparticles at sublethal concentrations disrupt cytoskeleton and neurite dynamics in cultured adult neural stem cells. Neurotoxicology, 48, 231-238.
Ka, M., et al. 2016. Microtubule-actin crosslinking factor 1 is required for dendritic arborization and axon outgrowth in the developing brain. Molecular Neurobiology, 53(9), pp.6018-6032.
Ghersa, F., et al. 2019. Reduced inflammatory state promotes reinnervation of endometriotic-like lesions in TNFRp55 deficient mice. Molecular Human Reproduction, 25(7), pp.385-396.
Product Specific Protocols
Tubulin, beta III Mouse Dentate Gyrus IHC Protocol: Download
Tubulin, beta III Mouse Hippocampus IHC Protocol: Download
