Name: Anti-Tubulin, beta III Antibody
Brand: PhosphoSolutions
SKU: 2020-TUB
Price: 415.0 USD
Availability: InStock

Our Anti-Tubulin, beta III primary antibody from PhosphoSolutions is mouse monoclonal. It detects mouse and rat Tubulin, beta III and is Protein G purified. It is great for use in WB, IHC, ICC.

Product Type

Primary Antibody

Species Reactivity

Rat

Expected Reactivity

Bovine, Canine, Feline, Goat, Guinea Pig, Hamster, Horse, Human, Non-Human Primate, Rabbit, Sheep, Vole

Clone

AA10

Applications

IHC, WB

Host Species

Mouse

Format

Protein G Purified

Gene Name

TUBB3

Molecular Weight

55 kDa

Go to Full Product Details

Immunostaining of mouse dentate gyrus showing beta III tubulin (red, 1:1000). The blue is staining nuclei. Photo courtesy of Robert Wine.

Western blot of rat cortical lysate showing specific immunolabeling of the ~55 kDa beta III tubulin protein.

Immunolabelling of the CA3 subfield of mouse hippocampus labeling β-III tubulin(cat. 2020-TUB, 1:1000, red) and CNP (cat. 325-CNP , green, 1:500). The blue is DAPI staining DNA. Photo courtesy of Rob Wine.

Immunostaining of mouse dentate gyrus showing beta III tubulin (red, 1:1000). The blue is staining nuclei. Photo courtesy of Robert Wine.

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-Tubulin, beta III Antibody

Product Details

Target

Tubulin, beta III

Target Description

Tubulin is the major constituent of microtubules, existing as a heterodimer of the α and β subunits. The beta III isoform of tubulin is found almost exclusively in neuronal processes of adult tissues and is therefore an excellent marker for neurons. Neuron specific, posttranslational modifications within the C-terminal domain of beta III tubulin have been shown to be developmentally regulated suggesting that they may serve to modulate the interaction of tubulin with microtubule associated proteins (Lee et al., 1990). Additionally, beta III tubulin has been found to be highly expressed in cancer cells such as small cell lung cancer, large cell neuroendocrine carcinoma and adenocarcinomas and is correlated with an increasing histological degree of malignancy (Katsetos et al., 2003)

Format

Protein G Purified

Clonality

Monoclonal

Clone

AA10

Isotype

IgG

Applications

IHC, WB

Host Species

Mouse

Gene Name

TUBB3

Molecular Weight

55 kDa

Antigen

Synthetic peptide corresponding to amino acid residues specific to rat beta III tubulin, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Rat

Expected Reactivity

Bovine, Canine, Feline, Goat, Guinea Pig, Hamster, Horse, Human, Non-Human Primate, Rabbit, Sheep, Vole

Antibody Registry ID

AB_2492275

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Protein G purified culture supernatant.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:10000
WB Brain: 1:10000
IHC: 1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~55 kDa beta III tubulin protein. This clone is similar to the monoclonal antibody Tuj1.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

beta 3 tubulin antibody, beta-4 antibody, beta III Tubulin, CDCBM antibody, CDCBM1 antibody, CFEOM3 antibody, CFEOM3A antibody, FEOM3 antibody, M(beta)3 antibody, M(beta)6 antibody, MC1R antibody, Neuron specific beta III Tubulin antibody, Neuron-specific class III beta-tubulin antibody, QccE-11995 antibody, QccE-15186 antibody, TBB3_HUMAN antibody, Tubb 3 antibody, TUBB3 antibody, TUBB4 antibody, Tubulin beta 3 antibody, Tubulin beta 3 chain antibody, Tubulin beta 4 antibody, Tubulin beta III antibody, Tubulin beta-3 chain antibody, Tubulin beta-4 chain antibody, Tubulin beta-III antibody

UniProt Details

UniProt (Human): Q13509
UniProt (Immunogen Species): Q4QRB4

NCBI Gene ID

246118

Product Specific References for Applications and Species

Immunocytochemistry: Rat
Immunohistochemistry: Mouse
Western Blotting: Mouse

Immunocytochemistry: Rat
PMID	Dilution	Publication
25952507	not listed	Cooper, R., et al. 2015. Silver nanoparticles at sublethal concentrations disrupt cytoskeleton and neurite dynamics in cultured adult neural stem cells. Neurotoxicology, 48, 231-238.

Immunohistochemistry: Mouse
PMID	Dilution	Publication
26526844	not listed	Ka, M., et al. 2016. Microtubule-actin crosslinking factor 1 is required for dendritic arborization and axon outgrowth in the developing brain. Molecular Neurobiology, 53(9), pp.6018-6032.

Western Blotting: Mouse
PMID	Dilution	Publication
26526844	1:8000	Ghersa, F., et al. 2019. Reduced inflammatory state promotes reinnervation of endometriotic-like lesions in TNFRp55 deficient mice. Molecular Human Reproduction, 25(7), pp.385-396.

Product Specific Protocols

Tubulin, beta III Mouse Dentate Gyrus IHC Protocol: Download

Tubulin, beta III Mouse Hippocampus IHC Protocol: Download

Western Blot Protocol: Download

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Anti-Tubulin, beta III Antibody (AA10)

Our Anti-Tubulin, beta III primary antibody from PhosphoSolutions is mouse monoclonal. It detects mouse and rat Tubulin, beta III and is Protein G purified. It is great for use in WB, IHC, ICC.

Anti-Tubulin, beta III Antibody
SKU: 2020-TUB

Bulk Order Anti-Tubulin, beta III Antibody

Product Details

Product Specific References for Applications and Species

Product Specific Protocols

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Anti-Tubulin, beta III Antibody (AA10)

Our Anti-Tubulin, beta III primary antibody from PhosphoSolutions is mouse monoclonal. It detects mouse and rat Tubulin, beta III and is Protein G purified. It is great for use in WB, IHC, ICC.

Anti-Tubulin, beta III Antibody SKU: 2020-TUB

Bulk Order Anti-Tubulin, beta III Antibody

Product Details

Product Specific References for Applications and Species

Product Specific Protocols

Anti-Tubulin, beta III Antibody
SKU: 2020-TUB