Our Anti-Trk B rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects rat Trk B and is affinity purified. It is great for use in WB.
Western blot of rat synaptic plasma membrane (SPM) lysate showing the specific immunolabeling of the ~93 kDa TrkB protein.
Tropomyosin-receptor-kinase (TRK) receptors are members of the receptor tyrosine kinase family and include TrkA, TrkB and TrkC. Each of these receptor types has different binding affinities to the neurotrophins nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF) and neurotrophin 3 (NT-3). TrkB has the highest affinity to binding BDNF which plays critical roles in the function and survival of neurons in the CNS (Soppet et al., 1991; Klein et al., 1991). Additionally, alterations in expression of TrkB have been associated with Alzheimer’s disease (Ferrer et al., 1999; Chen et al., 2008).
Affinity Purified
Polyclonal
IgG
WB
Rabbit
Ntrk2
93 kDa
Synthetic peptide corresponding to amino acid residues from the C-terminal region of rat TrkB, conjugated to keyhole limpet hemocyanin (KLH).
Rat
AB_2492266
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from rabbit serum by affinity purification on a column made with the C-terminal peptide used as antigen.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
WB Brain: 1:1000
Unconjugated
Specific for endogenous levels of the ~93 kDa TrkB protein in western blots.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Soppet D, Escandon E, Maragos J, Middlemas DS, Reid SW, Blair J, Burton LE, Stanton BR, Kaplan DR, Hunter T, Nikolics K, Parada LF (1991)The neurotrophic factors brain-derived neurotrophic factor and neurotrophin-3 are ligands for the trkB tyrosine kinase receptor. Cell, May 31;65(5):895-903. PMID: 1645620
Klein R, Nanduri V, Jing SA, Lamballe F, Tapley P, Bryant S, Cordon-Cardo C, Jones KR, Reichardt LF, Barbacid M (1991)The trkB tyrosine protein kinase is a receptor for brain-derived neurotrophic factor and neurotrophin-3. Cell, Jul 26;66(2):395-403. PMID: 1649702
Ferrer I, Marín C, Rey MJ, Ribalta T, Goutan E, Blanco R, Tolosa E, Martí E (1999) BDNF and full-length and truncated TrkB expression in Alzheimer disease. Implications in therapeutic strategies. Journal of Neuropathology and Experimental Neurology, Jul;58(7):729-39. PMID: 10411343
Chen Z, Simmons MS, Perry RT, Wiener HW, Harrell LE, Go RC. (2008) Genetic association of neurotrophic tyrosine kinase receptor type 2 (NTRK2) With Alzheimer's disease.American Journal of Medical Genetics Part B: Neuropsychiatric Genetics, Apr 5;147(3):363-9. PMID: 17918233
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