Our Anti-Synaptotagmin (Ser309) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects rat Synaptotagmin (Ser309) and is antigen affinity purified from pooled serum. It is great for use in WB, ICC.
Immunostaining of 14 DIV rat cortical neurons showing synaptotagmin when phosphorylated at Ser309 in red and PSD95 in green. Photo courtesy of Gang Liu.
Anti-Synaptotagmin (Ser309) Antibody
Synaptotagmin is widely regarded as the primary calcium sensor for synaptic vesicle exocytosis (Fernandez-Chacon et al., 2001; Wang et al., 2003). Moreover, recent studies indicate that the protein also plays a key role in endocytosis (Poskanzer et al., 2003). Synaptotagmin can be phosphorylated by multiple protein kinases and this may play a key role in modulation of synaptotagmin’s ability to influence both the exocytotic and endocytotic components of synaptic transmission (Hilfiker et al., 1999; Lee et al., 2004).
Antigen Affinity Purified from Pooled Serum
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser309 of rat synaptotagmin, conjugated to keyhole limpet hemocyanin (KLH).