Our Sphingosine Kinase 1 (C-terminal region) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Sphingosine Kinase 1 (C-terminal region) and is antigen affinity purified. It is great for use in WB.
Western blot image of recombinant his-tagged human SK1 protein. Blots were probed with rabbit polyclonals anti-SK1 (Central Region) (SP1621) at 1:1000 (lane 1) and 1:4000 (lane 2), and anti-SK1 (C-terminal region) (SP5421) at 1:1000 (lane 3) and 1:4000 (lane 4).
Sphingolipids are metabolized into bioactive products that include ceramide, sphingosine, and sphingosine-1-phosphate (S1P). Sphingosine Kinase (SK) catalyzes the phosphorylation of the lipid sphingosine, creating S1P. S1P subsequently signals through cell surface G protein-coupled receptors, as well as intracellularly, to modulate cell proliferation, survival, motility and differentiation. Two isoforms of SK have been identified, SK1 and SK2. The mRNA for both of these isoforms is widely expressed with SK1 expression highest in brain, heart, kidney, thymus, spleen and lung, while SK2 is highest in kidney and liver. SKs can be activated through growth factor, G protein-coupled, and immunoglobulin receptor signalling. SK1 has been shown to mediate cell growth, prevention of apoptosis, and cellular transformation, and is upregulated in a variety of human tumors. Regulation of SK1 may occur through ERK mediated phosphorylation of Ser-225. This phosphorylation leads to increased activity and translocation to the plasma membrane.
Antigen Affinity Purified
Polyclonal
IgG
WB
Rabbit
SPHK1
42
SK1 synthetic peptide (coupled to carrier) corresponding to amino acids in the C-terminal region of mouse SK1. This sequence has high homology to human and rat SK1, and is not homologous to sequences in SK2.
Human, Mouse, Rat
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
WB: 1:500
Unconjugated
The antibody detects 42 kDa* full-length recombinant human and mouse SK1 proteins, as well as detects 42 and 60 kDa forms of SK1 in human HeLa cells.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
United States
After date of receipt, stable for at least 1 year at -20°C.
Tan, J, et al. 2023. Ritanserin Suppresses Acute Myeloid Leukemia by Inhibiting DGKα to Downregulate Phospholipase D and the Jak-Stat/MAPK Pathway. Discover. Oncology, 118.
Nakamura, DS, et al. 2023. Expression and Subcellular Localization of Mitochondrial Docking Protein, Syntaphilin, in Oligodendrocytes and CNS Myelin Sheath. Glia, 2343-2355.
Bulk Order Anti-Sphingosine Kinase 1 (C-terminal region) Antibody
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