Anti-Sphingosine Kinase 1 (C-terminal region) Antibody

Name: Anti-Sphingosine Kinase 1 (C-terminal region) Antibody
Brand: PhosphoSolutions
SKU: SP5421
Price: 309.0 USD
Availability: InStock

from PhosphoSolutions

2 Citations

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Applications

Host Species

Rabbit

Format

Antigen Affinity Purified

Gene Name

SPHK1

Molecular Weight

Go to Full Product Details

The protein expression levels of DGKα, SphK1 (cat. SP5421, 1:1000) and p-SphK1(Ser225) (cat. SP1641) in AML cells after ritanserin treatment for 48 h. Image from publication CC-BY-4.0. PMID: 37392305

Western blot image of recombinant his-tagged human SK1 protein. Blots were probed with rabbit polyclonals anti-SK1 (Central Region) (SP1621) at 1:1000 (lane 1) and 1:4000 (lane 2), and anti-SK1 (C-terminal region) (SP5421) at 1:1000 (lane 3) and 1:4000 (lane 4).

Volume: 100 µL

Variant

Price:

$309

Quantity:

Bulk Order Anti-Sphingosine Kinase 1 (C-terminal region) Antibody

Product Details

Target

Sphingosine Kinase 1 (C-terminal region)

Target Description

Sphingolipids are metabolized into bioactive products that include ceramide, sphingosine, and sphingosine-1-phosphate (S1P). Sphingosine Kinase (SK) catalyzes the phosphorylation of the lipid sphingosine, creating S1P. S1P subsequently signals through cell surface G protein-coupled receptors, as well as intracellularly, to modulate cell proliferation, survival, motility and differentiation. Two isoforms of SK have been identified, SK1 and SK2. The mRNA for both of these isoforms is widely expressed with SK1 expression highest in brain, heart, kidney, thymus, spleen and lung, while SK2 is highest in kidney and liver. SKs can be activated through growth factor, G protein-coupled, and immunoglobulin receptor signalling. SK1 has been shown to mediate cell growth, prevention of apoptosis, and cellular transformation, and is upregulated in a variety of human tumors. Regulation of SK1 may occur through ERK mediated phosphorylation of Ser-225. This phosphorylation leads to increased activity and translocation to the plasma membrane.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

Host Species

Rabbit

Gene Name

SPHK1

Molecular Weight

Antigen

SK1 synthetic peptide (coupled to carrier) corresponding to amino acids in the C-terminal region of mouse SK1. This sequence has high homology to human and rat SK1, and is not homologous to sequences in SK2.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:500

Conjugate

Unconjugated

Specificity

The antibody detects 42 kDa* full-length recombinant human and mouse SK1 proteins, as well as detects 42 and 60 kDa forms of SK1 in human HeLa cells.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

SK1, SPHK1, SPK1

UniProt Details

UniProt (Human): Q9NYA1
UniProt (Immunogen Species): Q8CI15

Product Specific References for Applications and Species

Western Blot: Mouse | Rat

Western Blot: Mouse
PMID	Dilution	Publication
37392305	1:1000	Tan, J, et al. 2023. Ritanserin Suppresses Acute Myeloid Leukemia by Inhibiting DGKα to Downregulate Phospholipase D and the Jak-Stat/MAPK Pathway. Discover. Oncology, 118.

Western Blot: Rat
PMID	Dilution	Publication
37272718	1:500	Nakamura, DS, et al. 2023. Expression and Subcellular Localization of Mitochondrial Docking Protein, Syntaphilin, in Oligodendrocytes and CNS Myelin Sheath. Glia, 2343-2355.

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Anti-Sphingosine Kinase 1 (C-terminal region) Antibody