Name: Anti-Raf-1 (Ser642) Antibody
Brand: PhosphoSolutions
SKU: p1555-642
Price: 415.0 USD
Availability: InStock

Our Anti-Raf-1 (Ser642) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human and rat Raf-1 (Ser642) and is antigen affinity purified. It is great for use in WB.

Product Type

Primary Antibody

Species Reactivity

Human, Rat

Expected Reactivity

Bovine, Canine, Chicken, Mouse, Non-Human Primate

Applications

WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

RAF1

Molecular Weight

74 kDa

Go to Full Product Details

Western blot of HeLa lysate showing specific labeling of the ~74 kDa Raf 1 protein phosphorylated at Ser642 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-Raf-1 (Ser642) Antibody

Product Details

Target

Raf-1 (Ser642)

Target Description

The Ras pathway is a critical signal transduction cascade involved in regulating cellular proliferation, differentiation, survival, and oncogenic transformation. Members of the Raf serine/threonine kinase family are key intermediates in this cascade, functioning to relay signals from activated Ras to the downstream protein kinases MEK and ERK (Marshall, 1996). Previous studies have shown that phosphorylation is required for Raf-1 activation (Dhillon and Kolch, 2002; Chong et al., 2003). Recent work has demonstrated that phosphorylation also regulates the downregulation of Raf (Dougherty et al., 2005) with two sites participating: Ser-301 and Ser-642.

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

WB

Host Species

Rabbit

Gene Name

RAF1

Molecular Weight

74 kDa

Antigen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser642 of rat Raf-1, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Human, Rat

Expected Reactivity

Bovine, Canine, Chicken, Mouse, Non-Human Primate

Antibody Registry ID

AB_2492224

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~74 kDa Raf-1 protein phosphorylated at Ser642. Immunolabeling is completely eliminated by treatment with λ-phosphatase.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser642

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

c Raf antibody, C-raf antibody, C-Raf proto-oncogene serine/threonine kinase antibody, CMD1NN antibody, Craf 1 transforming gene antibody, cRaf antibody, Craf1 transforming gene antibody, EC 2.7.11.1 antibody, kinase Raf1 antibody, Murine sarcoma 3611 oncogene 1 antibody, NS5 antibody, Oncogene MIL antibody, Oncogene RAF1 antibody, OTTHUMP00000160218 antibody, OTTHUMP00000207813 antibody, OTTHUMP00000209389 antibody, Protein kinase raf 1 antibody, Proto-oncogene c-RAF antibody, Raf 1 antibody, Raf 1 proto oncogene serine/threonine kinase antibody, RAF antibody, Raf proto oncogene serine/threonine protein kinase antibody, RAF proto-oncogene serine/threonine-protein kinase antibody, RAF-1 antibody, RAF1 antibody, RAF1_HUMAN antibody, Similar to murine leukemia viral (V-raf-1) oncogene homolog 1 antibody, TRANSFORMING REPLICATION-DEFECTIVE MURINE RETROVIRUS 3611-MSV antibody, v raf 1 murine leukemia viral oncogene homolog 1 antibody, v-raf murine sarcoma viral oncogene homolog 1 antibody, v-raf-1 murine leukemia viral oncogene-like protein 1 antibody, vraf1 murine leukemia viral oncogene homolog 1 antibody

UniProt Details

UniProt (Human): P04049
UniProt (Immunogen Species): P11365

NCBI Gene ID

24703

Product Specific References for Applications and Species

Western Blot: Human

Western Blot: Human
PMID	Dilution	Publication
31911540	not listed	Stangl, C., et al. 2020. Diverse BRAF gene fusions confer resistance to EGFR-targeted therapy via differential modulation of BRAF activity. Molecular Cancer Research, 18(4), pp.537-548.

Product Specific Protocols

Western Blot Protocol: Download

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Anti-Raf-1 (Ser642) Antibody

Our Anti-Raf-1 (Ser642) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human and rat Raf-1 (Ser642) and is antigen affinity purified. It is great for use in WB.