Anti-Plexin A1 (Sema Domain) Antibody

Name: Anti-Plexin A1 (Sema Domain) Antibody
Brand: PhosphoSolutions
SKU: PP1471
Price: 329.0 USD
Availability: InStock

Our Plexin A1 (Sema Domain) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Plexin A1 (Sema Domain) and is antigen affinity purified. It is great for use in WB, ICC.

Immunocytochemical double labeling using anti-Myc mouse monoclonal and anti-Plexin-A1 rabbit polyclonal (PP1471) antibodies in Cos-7 cells mock transfected (A,D) or transfected with Myc-tagged mouse Plexin-A1 construct (B,E). The specificity of the binding in E was demonstrated by using Plexin-A1 peptide (PX1475) in the presence of this anti-Plexin-A1 antibody (C,F).

Western blots showing mouse brain (lanes 1 & 4), and Cos-7 cells untransfected (lanes 2 & 5) or transfected with mouse myc-tagged Plexin-A1 (lanes 3 & 6). The blots were probed with either affinity purified anti-Plexin-A1 (PP1471); lanes 1-3) or with mouse monoclonal anti-Myc (lanes 4-6).

Species Reactivity

Human, Mouse, Rat

Applications

ICC, WB

Host Species

Rabbit

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from PhosphoSolutions

SKU: PP1471

Volume: 100 µL

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Price:

$329

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Product Details

Target

Plexin A1 (Sema Domain)

Target Description

Plexins are a family of large integral membrane proteins that complex with neuropilins to form semaphorin co-receptors. The extracellular region of plexins contains a semaphorin domain, multiple glycine-rich motifs, and MET-related sequences. The cytoplasmic region contains a Sex/Plexin domain and putative tyrosine phosphorylation sites that mediate signal transduction after activation. This region in Plexin-A1 binds the RhoGTPases, Rnd1 and RhoD. Recruitment of Rnd1 has been implicated in the cytoskeletal collapse that occurs after semaphorin-mediated activation of Plexin-A1, while RhoD may block this collapsing activity through interaction with the cytoplasmic region of Plexin-A1. The expression of Plexin-A1, along with the co-receptor Neuropilin-1, is upregulated in neurons after central nervous system injury. The axons from these neurons cannot cross semaphorin 3A-containing regions at the site of injury. Thus, semaphorin 3A and its co-receptors, Plexin-A1 and Neuropilin-1, may have significant roles in axon regeneration after neuronal injury.<

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, WB

Host Species

Rabbit

Gene Name

PLXNA1

Molecular Weight

220

Antigen

Plexin-A1 synthetic peptide (coupled to carrier protein) corresponding to a region within the semaphorin domain in human Plexin-A1. This sequence is highly conserved in rat and mouse Plexin-A1, and has low homology to other Plexin family members.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:500
ICC: 1:50

Conjugate

Unconjugated

Specificity

The antibody detects a 220 kDa* protein corresponding to the apparent molecular mass of Plexin-A1 on SDS-PAGE immunoblots of adult mouse brain and Cos-7 cells transfected with mouse Plexin-A1. In immunocytochemistry, this antibody co-localizes with anti-Myc in Cos-7 cells transfected with Myc-tagged mouse Plexin-A1.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

PLXN1, NOV, Sema3A

UniProt Details

UniProt (Human): Q9UIW2
UniProt (Immunogen Species): Q9UIW2

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Anti-Plexin A1 (Sema Domain) Antibody

Our Plexin A1 (Sema Domain) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Plexin A1 (Sema Domain) and is antigen affinity purified. It is great for use in WB, ICC.

SKU: PP1471

Product Details

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