Anti-PAK6 (Ser-165), Phosphospecific Antibody

Name: Anti-PAK6 (Ser-165), Phosphospecific Antibody
Brand: PhosphoSolutions
SKU: PP1551
Price: 309.0 USD
Availability: InStock

from PhosphoSolutions

Product Type

Primary Antibody

Species Reactivity

Human

Applications

Host Species

Rabbit

Format

Antigen Affinity Purified

Gene Name

PAK6

Molecular Weight

Go to Full Product Details

Western blots of human PAK6 recombinant protein phosphorylated by ERK2. The blot was exposed to lambda phosphatase (lanes 2 & 6) then probed with anti-PAK6 (N-terminal) (lanes 1-4) or anti-PAK6 (Ser-165) phospho-specific (lanes 5-8). The antibodies were used in the presence of unrelated (lane 3) and PAK6 (N-terminal) (lane 4) peptide or PAK6 (Ser-165) (lane 7) and unrelated phospho-serine (lane 8) peptides, respectively.

Volume: 100 µL

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Product Details

Target

PAK6 (Ser-165)

Target Description

p21-activated kinase 6 (PAK6) is a member of the PAK family of serine/threonine kinases. These kinases have a highly conserved amino-terminal Cdc42/Rac interactive binding domain and a carboxyl-terminal kinase domain. PAK kinases are implicated in the regulation of a number of cellular processes, including cytoskeleton rearrangement, apoptosis and the MAP kinase signaling pathway. PAK6 interacts with the androgen receptor, a steroid hormone transcription factor involved in male sexual differentiation and development. PAK6 is highly expressed in testis and prostate tissues. Regulation of PAK6 kinase activity occurs through multiple sites of phosphorylation. Activation of PAK6 requires autophosphorylation of Ser-560 and MKK-6 induced phosphorylation of Tyr-566. In addition, p38 MAPK can phosphorylate Ser-165, which increases PAK6 kinase activity. Thus, multiple signaling pathways may regulate the activity of PAK6 through differential phosphorylation.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

Host Species

Rabbit

Gene Name

PAK6

Molecular Weight

Antigen

PAK6 (Ser-165) synthetic peptide (coupled to carrier protein) corresponding to amino acids around serine 165 in human PAK6. The sequence used has two amino acid differences compared to rat and mouse PAK6.

Species Reactivity

Human

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

This antibody was cross-adsorbed to unphosphorylated PAK6 (Ser-165) peptide then affinity purified using phospho-PAK6 (Ser-165) peptide. The antibody detects a 75 kDa* band on SDS-PAGE immunoblots of human PAK6 recombinant protein phosphorylated with ERK2. This reactivity is specifically blocked by pre-incubation with phospho-PAK6 (Ser-165) peptide and also by treatment with lambda phosphatase.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser-165

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

p21, PAK

UniProt Details

UniProt (Human): Q9NQU5
UniProt (Immunogen Species): Q9NQU5

Product Specific Protocols

Western Blot Protocol: Download

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Anti-PAK6 (Ser-165), Phosphospecific Antibody