Name: Anti-NMDA NR2B Subunit (Tyr1472) Antibody
Brand: PhosphoSolutions
SKU: p1516-1472
Price: 415.0 USD
Availability: InStock

Our Anti-NMDA NR2B Subunit (Tyr1472) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat NMDA NR2B Subunit (Tyr1472) and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Type

Primary Antibody

Species Reactivity

Mouse, Rat

Expected Reactivity

Bovine, Canine, Chicken, Human, Non-Human Primate, Zebrafish

Applications

WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

GRIN2B

Molecular Weight

180 kDa

Go to Full Product Details

Western blot of rat hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2B subunit of the NMDAR phosphorylated at Tyr1472 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (400 units/100uL lysate for 30 min).

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-NMDA NR2B Subunit (Tyr1472) Antibody

Product Details

Target

NMDA NR2B Subunit (Tyr1472)

Target Description

The ion channels activated by glutamate that are sensitive to N-methyl-Daspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989; Alvestad et al., 2003; Snell et al., 1996). Channels with physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits (Ishii et al., 1993). Overexpression of the NR2B-subunit of the NMDA Receptor has been associated with increases in learning and memory while aged, memory impaired animals have deficiencies in NR2B expression (Clayton et al., 2002a; Clayton et al., 2002b). Recent work suggests that phosphorylation of Tyr-1472 on NR2B may regulate the functional expression the receptor in LTP and other forms of plasticity (Nakazawa et al., 2001; Roche et al., 2001).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

WB

Host Species

Rabbit

Gene Name

GRIN2B

Molecular Weight

180 kDa

Antigen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr1472 of the NR2B subunit of the rat NMDA receptor, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Mouse, Rat

Expected Reactivity

Bovine, Canine, Chicken, Human, Non-Human Primate, Zebrafish

Antibody Registry ID

AB_2492182

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000
ICC: 1:100

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~180 kDa NMDAR NR2B-subunit protein phosphorylated at Tyr1472. Immunolabeling is completely eliminated by treatment with λ-phosphatase.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Tyr1472

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

EPND antibody, FESD antibody, GluN2A antibody, Glutamate [NMDA] receptor subunit epsilon-1 antibody, Glutamate receptor antibody, Glutamate receptor ionotropic N methyl D aspartate 2A antibody, GRIN 2A antibody, GRIN2A antibody, hNR2A antibody, LKS antibody, N methyl D aspartate receptor channel subunit epsilon 1 antibody, N Methyl D Aspartate Receptor Subtype 2A antibody, N methyl D aspartate receptor subunit 2A antibody, N-methyl D-aspartate receptor subtype 2A antibody, NMDA receptor subtype 2A antibody, NMDAR 2A antibody, NMDAR2A antibody, NMDE1_HUMAN antibody, NR2A antibody, OTTHUMP00000160135 antibody, OTTHUMP00000174531 antibody

UniProt Details

UniProt (Human): Q13224
UniProt (Immunogen Species): Q00960

NCBI Gene ID

24410

Product Specific References for Applications and Species

Immunocytochemistry: Rat
Western Blot: Human | Mouse | Rat

Immunocytochemistry: Rat
PMID	Dilution	Publication
32651756	1:100	Akkuratov, E.E., et al. 2020. Ouabain Modulates the Functional Interaction Between Na, K-ATPase and NMDA Receptor. Molecular Neurobiology, pp.1-13.

Western Blot: Mouse
PMID	Dilution	Publication
31135041	1:1000	Dedek, A., et al. 2019. Loss of STEP61 couples disinhibition to N-methyl-d-aspartate receptor potentiation in rodent and human spinal pain processing. Brain, 142(6), pp.1535-1546.

Western Blot: Mouse
PMID	Dilution	Publication
34044147	1:1000	Zhang, L., et al. 2021. Tyrosine phosphatase PTP1B impairs presynaptic NMDA receptor-mediated plasticity in a mouse model of Alzheimer's disease. Neurobiology of Disease, 156, p.105402.
31543505	1:1000	Ajit, D., et al. 2020. A unique tau conformation generated by an acetylation-mimic substitution modulates P301S-dependent tau pathology and hyperphosphorylation. Journal of Biological Chemistry, 294(45), pp.16698-16711.
30518596	1:1000	Salazar, S.V., et al. 2019. Alzheimer's disease risk factor Pyk2 mediates amyloid-β-induced synaptic dysfunction and loss. Journal of Neuroscience, 39(4), pp.758-772.
31452242	1:1000	Liu, G., et al. 2019. Loss of tau and Fyn reduces compensatory effects of MAP2 for tau and reveals a Fyn‐independent effect of tau on calcium. Journal of neuroscience research. Aug 26.
30551522	not listed	Zhang, X., et al. 2019. Effects of 5-Aza on p-Y1472 NR2B related to learning and memory in the mouse hippocampus. Biomedicine & Pharmacotherapy, 109, pp.701-707.
27335408	not listed	Xiao, X., et al. 2016. Disruption of Coordinated Presynaptic and Postsynaptic Maturation Underlies the Defects in Hippocampal Synapse Stability and Plasticity in Abl2/Arg-Deficient Mice. The Journal of Neuroscience, 36(25), 6778-6791.
27094400	1:1000	Zamzow, D.R., et al. 2016. Higher levels of phosphorylated Y1472 on GluN2B subunits in the frontal cortex of aged mice are associated with good spatial reference memory, but not cognitive flexibility. AGE, 38(3), 1-17.
22544749	1:1000	Xu, J., et al. 2012. Striatal-enriched protein-tyrosine phosphatase (STEP) regulates Pyk2 kinase activity. J Biol Chem. Jun 15;287(25):20942-56.
22523092	1:500	Gladding, C.M., et al. 2012. Calpain and Striatal-Enriched protein tyrosine phosphatase (STEP) activation contribute to extrasynaptic NMDA receptor localization in a Huntington’s disease mouse model. Hum Mol Genet. Sep 1;21(17):3739-52.
21464302	1:1000	Hinklin, T.R., et al. 2011. Alcohol inhibition of the NMDA receptor function, long-term potentiation, and fear learning requires striatal-enriched protein tyrosine phosphatase. PNAS, Apr 2011; 108: 6650 - 6655.
20427654	1:1000	Kurup, P., et al. 2010. Aβ-mediated NMDA receptor endocytosis in Alzheimer's disease involves ubiquitination of the tyrosine phosphatase STEP61. Journal of Neuroscience, 30(17), pp.5948-5957.

Western blot: Rat
PMID	Dilution	Publication
34653560	1:1500	Su, L., et al. 2021. . P2Y1 purinergic receptor inhibition attenuated remifentanil-induced postoperative hyperalgesia via decreasing NMDA receptor phosphorylation in dorsal root ganglion. Brain Research Bulletin, 177, pp.352-362.
34166321	1:1000	Su, L., et al. 2021. . P2Y1 Purinergic Receptor Contributes to Remifentanil-Induced Cold Hyperalgesia via Transient Receptor Potential Melastatin 8–Dependent Regulation of N-methyl-d-aspartate Receptor Phosphorylation in Dorsal Root Ganglion. Anesthesia & Analgesia, 133(3), pp.794-810.
33257326	not listed	Higginbotham, J.A., et al. 2021. . CB1 receptor signaling modulates amygdalar plasticity during context-cocaine memory reconsolidation to promote subsequent cocaine seeking. Journal of Neuroscience, 41(4), pp.613-629.
32437889	1:500	Zhu, D., et al. 2020. . Angiotensin (1-7) through modulation of the NMDAR–nNOS–NO pathway and serotonergic metabolism exerts an anxiolytic-like effect in rats. Behavioral Brain Research, 390, p.112671.
27127657	not listed	Jang, S.S., et al. 2016. Seizure-Induced Regulations of Amyloid-β, STEP61, and STEP61 Substrates Involved in Hippocampal Synaptic Plasticity. Neural plasticity, 2016.
26391783	not listed	Jang, S.S., et al. 2015. Regulation of STEP61 and tyrosine-phosphorylation of NMDA and AMPA receptors during homeostatic synaptic plasticity. Molecular brain, 8.
26277342	not listed	Mao, L.M., et al. 2015. Dopaminergic and cholinergic regulation of Fyn tyrosine kinase phosphorylation in the rat striatum in vivo. Neuropharmacology, 99, 491-499.
24611998	1:1000	Chen, W., et al. 2014. BDNF released during neuropathic pain potentiates NMDA receptors in primary afferent terminals. European Journal of Neuroscience, 39(9), 1439-1454.
21464302	1:1000	Hinklin, T.R., et al. 2011. Alcohol inhibition of the NMDA receptor function, long-term potentiation, and fear learning requires striatal-enriched protein tyrosine phosphatase. PNAS, Apr 2011; 108: 6650 - 6655.
21215796	1:1000	Castillo, C., et al. 2011. The N-methyl-D-aspartate-evoked cytoplasmic calcium increase in adult rat dorsal root ganglion neuronal somata was potentiated by substance P pretreatment in a protein kinase C-dependent manner. Neuroscience, 177, pp.308-320.
20427654	1:1000	Kurup, P., et al. 2010. Aβ-mediated NMDA receptor endocytosis in Alzheimer's disease involves ubiquitination of the tyrosine phosphatase STEP61. Journal of Neuroscience, 30(17), pp.5948-5957.
19625523	1:1000	Xu, J., et al. 2009. Extrasynaptic NMDA receptors couple preferentially to excitotoxicity via calpain-mediated cleavage of STEP. Journal of Neuroscience, 29(29), pp.9330-9343.
12536146	1:1000	Alvested, R.M., et al. 2003. Tyrosine dephosphorylation and ethanol inhibition of N-methyl-D-aspartate receptor function. Journal of Biological Chemistry, 278(13), pp.11020-11025.

Product Specific Protocols

Western Blot Protocol: Download

Recover password

Create my account

Anti-NMDA NR2B Subunit (Tyr1472) Antibody

Our Anti-NMDA NR2B Subunit (Tyr1472) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat NMDA NR2B Subunit (Tyr1472) and is antigen affinity purified from pooled serum. It is great for use in WB.