Name: Anti-Munc-18 (Ser 513) Antibody
Brand: PhosphoSolutions
SKU: p1305-515
Price: 415.0 USD
Availability: InStock

Our Anti-Munc-18 (Ser 513) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects rat Munc-18 (Ser 513) and is antigen affinity purified from pooled serum. It is great for use in WB, ICC.

Product Type

Primary Antibody

Species Reactivity

Rat

Expected Reactivity

Bovine, Canine, Chicken, Human, Mouse, Non-Human Primate, Xenopus

Applications

WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

STXBP2

Molecular Weight

65 kDa

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Immunostaining of 14 DIV rat cortical neurons showing specific labeling of Munc-18 phosphorylated at Ser513 (cat. p1305-515, 1:400,m red) and PSD95 (green).

Western blot of rat cortical lysate showing specific immunolabeling of the ~65 kDa Munc-18 protein phosphorylated at Ser513 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).

Volume: 100 µL

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Price:

$415

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Product Details

Target

Munc-18 (Ser 513)

Target Description

Munc-18 (mammalian homologue of Unc-18) is a protein that is thought to be involved in regulating exocytosis due, at least in part, to it ability to bind syntaxin (Ciufo et al., 2005). Munc18-1 is a neuron-specific member of the Sec1/Munc18 protein family that binds to syntaxin1A and is thought to stabilize the complex (Liu et al., 2004). The function of Unc-18 is thought to be regulated by PKC phosphorylation of Ser-515 in C. Elegans (Sassa et al., 1996). Ser-513 is the mammalian homologue of Ser-515 in C. Elegans.

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

WB

Host Species

Rabbit

Gene Name

STXBP2

Molecular Weight

65 kDa

Antigen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser513 of rat Munc-18, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Rat

Expected Reactivity

Bovine, Canine, Chicken, Human, Mouse, Non-Human Primate, Xenopus

Antibody Registry ID

AB_2492156

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000
ICC: 1:400

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~65 kDa Munc-18 protein phosphorylated at Ser513. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser513

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

FLJ37475 antibody, Munc 18 1 antibody, Munc 18a antibody, MUNC18 1 antibody, N-Sec1 antibody, Neuronal SEC1 antibody, NSec1 antibody, p67 antibody, Protein unc-18 homolog 1 antibody, Protein unc-18 homolog A antibody, Rb sec1 antibody, RBSEC1 antibody, STXB1_HUMAN antibody, STXBP1 antibody, Syntaxin binding protein 1 antibody, Syntaxin-binding protein 1 antibody, Unc 18 homolog antibody, Unc 18A antibody, Unc-18A antibody, Unc18 1 antibody, UNC18 antibody, Unc18-1 antibody

UniProt Details

UniProt (Human): Q15833
UniProt (Immunogen Species): Q62753

NCBI Gene ID

81804

Product Specific References for Applications and Species

Western Blot: Rat

Western Blot: Rat
PMID	Dilution	Publication
18519737	not listed	Leal-Ortiz, S., et al. 2008. Piccolo modulation of Synapsin1a dynamics regulates synaptic vesicle exocytosis. Journal of Cell Biology, 181: 831- 846.

Product Specific Protocols

Western Blot Protocol: Download

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Anti-Munc-18 (Ser 513) Antibody

Our Anti-Munc-18 (Ser 513) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects rat Munc-18 (Ser 513) and is antigen affinity purified from pooled serum. It is great for use in WB, ICC.