Anti-MeCP2 (Ser-421), Phosphospecific Antibody

Name: Anti-MeCP2 (Ser-421), Phosphospecific Antibody
Brand: PhosphoSolutions
SKU: MP4611
Price: 309.0 USD
Availability: InStock

from PhosphoSolutions Datasheet

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Applications

ICC, WB

Host Species

Rabbit

Format

Antigen Affinity Purified

Gene Name

MECP2

Molecular Weight

Go to Full Product Details

Immunocytochemical labeling of MeCP2 phosphorylation in rat PC12 cells differentiated with NGF. The cells were probed with MeCP2 (Ser-421) rabbit polyclonal antibody (MP4611) in the absence (left) or presence (right) of blocking peptide (MX4615). The antibody was detected using appropriate secondary antibody conjugated to DyLight® 594.

Western blot of human PC3 cells treated with calyculin A (25 mM) for 15 min. The blot lanes were untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4) then probed with rabbit polyclonals anti-MeCP2 (C-terminus) (lanes 1 & 2) or anti-MeCP2 (Ser-421) (lanes 3 & 4).

Volume: 100 µL

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$309

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Product Details

Target

MeCP2 (Ser-421)

Target Description

Methyl-CpG Binding Protein 2 (MeCP2) was identified based on its affinity for methylated cytosines within DNA. As a chromatin-associated multifunctional protein, MeCP2 has been implicated in regulation of transcription and chromatin structure. Mutations of MeCP2 cause Rett syndrome, which results from neuronal dysfunction and impairment in cognitive and motor functions. Regulation of MeCP2 activity may involve phosphorylation at multiple sites. Ser-421 in MeCP2 is phosphorylated in response to neuronal activity, calcium influx, and is dependent on Cam-KII. Alanine mutation of Ser-421 leads to defects in synapse development and activity. Ser-80 in MeCP2 is phosphorylated in HeLa nuclear extracts and neurons. Alanine mutation of Ser-80 attenuates MeCP2 chromatin association and leads to locomotor deficits in transgenic knock-in mice. Thus, phosphorylation of MeCP2 may be important for altering its function during neuronal activity.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, WB

Host Species

Rabbit

Gene Name

MECP2

Molecular Weight

Antigen

phospho-MeCP2 (Ser-421) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding serine 421 in mouse MeCP2. This peptide sequence is highly conserved in rat and human MeCP2, and has low homology to other nuclear proteins.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:500
ICC: 1:250

Conjugate

Unconjugated

Specificity

The antibody detects a 75 kDa* protein corresponding to the molecular mass of MeCP2 on SDS-PAGE immunoblots of human PC3 cells treated with calyculin A and in mouse brain tissue. These reactivities are not observed after lambda phosphatase treatment.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser-421

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Methyl-CpG-Binding2

UniProt Details

UniProt (Human): P51608
UniProt (Immunogen Species): Q9Z2D6

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Anti-MeCP2 (Ser-421), Phosphospecific Antibody