Our Anti-MARCKS (Ser152,156) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat MARCKS (Ser152,156) and is antigen affinity purified from pooled serum. It is great for use in WB.
Western blot of rat brain lysate showing specific immunolabeling of the ~87 kDa MARCKS protein phosphorylated at Ser152,156, in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).
Myristoylated Alanine-Rich C Kinase Substrate (MARCKS) is a major substrate for phosphorylation by protein kinase C (PKC) (Ouimet et al., 1990). The phosphorylation of Ser-152/156 can be used as a measure of PKC activation although these sites are also phosphorylated by PRK1 (Palmer et al., 1996) MARCKS is a member of a family of calmodulin binding proteins and phosphorylation of Ser-152/156 modulates the binding of MARCKS to calmodulin (Verghese et al., 1994)
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
WB
Rabbit
MARCKS
87 kDa
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser152/156 of rat MARCKS, conjugated to keyhole limpet hemocyanin (KLH).
Mouse, Rat
Bovine, Chicken, Human, Xenopus, Zebrafish
AB_2492143
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
Unconjugated
Specific for endogenous levels of the ~87 kDa MARCKS protein phosphorylated at Ser152 and Ser156. Immunolabeling is completely eliminated by treatment with λ-phosphatase.
Phosphorylated
Ser152,156
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
80 kDa protein antibody, 80K L antibody, 80K L protein antibody, 80K-L protein antibody, 80KL antibody,81 kDa protein, light chain antibody, light chain antibody, MACS antibody, MARCKS antibody, MARCS antibody, MARCS_HUMAN antibody, MGC52672 antibody, myristoylated alanine rich C kinase substrate antibody, Myristoylated alanine rich protein kinase C substrate (MARCKS, 80K L) antibody, Myristoylated alanine rich protein kinase C substrate antibody, Myristoylated alanine-rich C-kinase substrate antibody, Phosphomyristin antibody, PKCSL antibody, PRKCSL antibody, protein kinase C substrate 80 kDa protein light chain antibody, Protein kinase C substrate antibody
Betti, M., et al. 2011. Maternal dietary loads of α-tocopherol depress protein kinase C signaling and synaptic plasticity in rat postnatal developing hippocampus and promote permanent deficits in adult offspring. The Journal of Nutritional Biochemistry, 22(1), pp.60-70.
