Anti-LRRK2/Dardarin, N-Terminus Antibody (8G10)

Name: Anti-LRRK2/Dardarin, N-Terminus Antibody
Brand: NeuroMab
SKU: 75-308
Price: 374.0 USD
Availability: InStock

Our Anti-LRRK2/Dardarin, N-terminus mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone 8G10. It is KO validated, detects human, mouse, and rat LRRK2/Dardarin, N-terminus, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Immunoblot versus crude membranes from adult rat brain (RBM) and wild-type (WT) and LRRK2 KO mouse brains probed with 8G10 (left), N241A/34 (middle) and K89/34 (right) TC supe. Mouse brain samples provided by Xiaojie Li, Ted Dawson and Valina Dawson, Johns Hopkins University

Adult rat brain immunohistochemistry (with antigen retrieval via sodium citrate pretreatment).

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Mouse

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Data Sheet

KO Validated

SKU: 75-308

Product Details

Target

Leucine-rich repeat serine/threonine-protein kinase 2 (EC 2.7.11.1) (Dardarin)

Target Description

LRRK2 (also known as PARK8) encodes a protein with 5 putative functional domains: an N-terminal leucine-rich repeat (LRR) domain, a Roc (Ras of complex protein) domain that shares sequence homology to the Ras-related GTPase superfamily, a COR (C-terminal of Roc) domain, a mitogen-activated protein kinase kinase kinase (MAPKKK) domain, and a C-terminal WD40 repeat domain. Mutation in this gene is one of the most common causes of inherited Parkinson disease (Gandhi et al., 2008). LRRK2 was originally identified as a putative disease-causing transcript (DKFZp434H2111) within a 2.6-Mb region encompassing a locus for Parkinson disease-8 (PARK8). Northern blot analysis detected a 9-kb mRNA transcript in all tissues tested, including brain. The authors named the protein product dardarin, derived from the Basque word dardara, meaning tremor. LRRK2/dardarin is also known to positively regulate autophagy through a calcium-dependent activation of the CaMKK/AMPK signaling pathway and together with RAB29, plays a role in the retrograde trafficking pathway for recycling proteins, such as mannose 6 phosphate receptor (M6PR), between lysosomes and the Golgi apparatus in a retromer-dependent manner. LRRK2/PARK8 is also known to regulate neuronal process morphology in the intact central nervous system (CNS) and play a role in synaptic vesicle trafficking.

Format

Purified

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

8G10

Isotype

IgG1

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

LRRK2 PARK8

Molecular Weight

>200 kDa

Antigen

Fusion protein amino acids 100-500 (N-terminus) of human LRRK2 (also known as Leucine-rich repeat serine/threonine-protein kinase 2, Dardarin and PARK8, accession number Q5S007); Mouse: 82% identity (332/401 amino acids identical); Rat: 82% identity (330/401 amino acids identical); 30% identity with LRRK1; This NeuroMab antibody is considered "Restricted" and is therefore not available for commercial re-distribution on a for-profit basis.

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2315882

Storage

Store at ≤ -20 C for long term storage. For short term storage, store at 2-8 C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Buffer

10mM Tris, 50mM Sodium Chloride, 0.065 % Sodium Azide pH 7.4

Conjugate

Unconjugated

Quality Control

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from opening

Synonyms

Leucine-rich repeat serine/threonine-protein kinase 2 (EC 2.7.11.1) (Dardarin)

UniProt Details

UniProt (Human): Q5S007
UniProt (Immunogen Species): Q5S007

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Anti-LRRK2/Dardarin, N-Terminus Antibody (8G10)

SKU: 75-308

Product Details

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