Name: Anti-Hsp60 Antibody
Brand: PhosphoSolutions
SKU: 750-HSP60
Price: 415.0 USD
Availability: InStock

Our Anti-Hsp60 primary antibody from PhosphoSolutions is mouse monoclonal. It detects human, mouse, and rat Hsp60 and is Protein G purified. It is great for use in WB.

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Expected Reactivity

Bovine, Canine, Feline, Goat, Guinea Pig, Hamster, Horse, Non-Human Primate, Rabbit, Sheep, Zebrafish

Clone

1C7

Applications

WB

Host Species

Mouse

Format

Protein G Purified

Gene Name

HSPD1

Molecular Weight

60 kDa

Go to Full Product Details

Immunostaining of HeLa cells showing strong mitochondrial labeling of HSP60 (cat. 750-HSP60, 1:5000, red) and nuclear staining with DAPI (blue).

Immunofluorescence of HeLa cells showing strong mitochondrial staining of HSP60(cat. 750-HSP60, 1:500) in red.

Western blot of HeLa cell lysate showing specific immunolabeling of the ~60 kDa HSP60 protein.

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-Hsp60 Antibody

Product Details

Target

Hsp60

Target Description

HSP60 is a member of the heat shock protein family which is thought to play a key role in mitochondrial protein transport and proper refolding of proteins imported from the cytoplasm into the mitochondrial matrix (Koll et al., 1992). More recently, HSP60 has been implicated in the pathogenesis of cardiovascular disease (Rizzo et al., 2011).

Format

Protein G Purified

Clonality

Monoclonal

Clone

1C7

Isotype

IgG1

Applications

WB

Host Species

Mouse

Gene Name

HSPD1

Molecular Weight

60 kDa

Antigen

Recombinant full length human HSP60 protein.

Species Reactivity

Human, Mouse, Rat

Expected Reactivity

Bovine, Canine, Feline, Goat, Guinea Pig, Hamster, Horse, Non-Human Primate, Rabbit, Sheep, Zebrafish

Antibody Registry ID

AB_2715493

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Protein G purified culture supernatant

Buffer

PBS + 50% glycerol and 5 mM NaN3

Dilution Ranges

WB: 1:1000
IHC: 1:1000-1:5000
ICC: 1:500-1:5000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~ 60 kDa HSP60 protein.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

60 kDa chaperonin antibody, 60 kDa heat shock protein, mitochondrial antibody, CH60_HUMAN antibody, Chaperonin 60 antibody, Chaperonin, 60-KD antibody, CPN60 antibody, fa04a05 antibody, GROEL antibody, heat shock 60kDa protein 1 (chaperonin) antibody, Heat shock protein 1 (chaperonin) antibody, Heat shock protein 60 antibody, Heat shock protein 65 antibody, heat shock protein family D (Hsp60) member 1 antibody, HLD4 antibody, Hsp 60 antibody, HSP 65 antibody, HSP-60 antibody, HSP60 antibody, HSP65 antibody, HSPD1 antibody, HuCHA60 antibody, Mitochondrial matrix protein P1 antibody, P60 lymphocyte protein antibody, short heat shock protein 60 Hsp60s1 antibody, SPG13 antibody

UniProt Details

UniProt (Human): P10809
UniProt (Immunogen Species): P10809

NCBI Gene ID

3329

Product Specific Protocols

Western Blot Protocol: Download

General References

Koll, H., Guiard, B., Rassow, J., Ostermann, J., Horwich, A.L., Neupert, W. and Hartl, F.U., 1992. Antifolding activity of hsp60 couples protein import into the mitochondrial matrix with export to the intermembrane space. Cell, 68(6), pp.1163-1175. PMID: 1347713
Rizzo, M., JL Macario, A., Conway de Macario, E., Gouni-Berthold, I., K Berthold, H., Battista Rini, G., Zummo, G. and Cappello, F., 2011. Heat shock protein-60 and risk for cardiovascular disease. Current Pharmaceutical Design, 17(33), pp.3662-3668. PMID: 22074436

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Anti-Hsp60 Antibody (1C7)

Our Anti-Hsp60 primary antibody from PhosphoSolutions is mouse monoclonal. It detects human, mouse, and rat Hsp60 and is Protein G purified. It is great for use in WB.

Anti-Hsp60 Antibody
SKU: 750-HSP60

Bulk Order Anti-Hsp60 Antibody

Product Details

Product Specific Protocols

General References

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Anti-Hsp60 Antibody (1C7)

Our Anti-Hsp60 primary antibody from PhosphoSolutions is mouse monoclonal. It detects human, mouse, and rat Hsp60 and is Protein G purified. It is great for use in WB.

Anti-Hsp60 Antibody SKU: 750-HSP60

Bulk Order Anti-Hsp60 Antibody

Product Details

Product Specific Protocols

General References

Anti-Hsp60 Antibody
SKU: 750-HSP60