Anti-Girdin (C-terminus) Antibody (M012)

Name: Anti-Girdin (C-terminus) Antibody
Brand: PhosphoSolutions
SKU: GM0121
Price: 309.0 USD
Availability: InStock

from PhosphoSolutions Datasheet

Product Type

Primary Antibody

Species Reactivity

Human

Clone

M012

Applications

ICC, IHC, IP, WB

Host Species

Mouse

Format

Protein G Purified

Gene Name

CCDC88A

Molecular Weight

250

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Immunocytochemical labeling of Girdin in aldehyde fixed and NP-40 permeabilized human NCI-H1915 lung carcinoma cells. The cells
were labeled with mouse monoclonal anti-Girdin (GM0121). The antibody was detected using goat anti-mouse DyLight® 594.

Western blot image of human A431 cell lysates treated with pervanadate (lanes 1-4). The blot was treated with alkaline phosphatase to dephosphorylate Girdin phosphosites (lanes 2 & 4). The blot was probed with mouse monoclonal anti-Girdin (lanes 1 & 2) or rabbit polyclonal anti-Girdin (Tyr-1764), phospho-specific (lanes 3 & 4).

Volume: 100 µL

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$309

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Product Details

Target

Girdin (C-terminus)

Target Description

Girdin, a member of the CCDC88 (Hook related protein) family, is an actin binding protein involved with cell migration and maintaining cytoskeletal organization. Girdin has conserved domains at the N- and C-terminus that bind microtubules and actin, respectively. It enhances PI3-kinase dependent phosphorylation of proteins, most notably Akt. This same activity can contribute to tumor proliferation, invasion, and metastasis in breast, ovarian, prostate, and pancreatic tissues. Girdin is phosphorylated at three separate locations: Ser-1416, Ser-1674, and Tyr-1764. Ser-1416 is the primary Akt phosphorylation site, while Cyclin-dependent kinases interact with Girdin and phosphorylate Ser-1674. Multiple receptor tyrosine kinases can bind girdin and phosphorylate Tyr-1764.

Format

Protein G Purified

Clonality

Monoclonal

Clone

M012

Isotype

IgG1

Applications

ICC, IHC, IP, WB

Host Species

Mouse

Gene Name

CCDC88A

Molecular Weight

250

Antigen

Clone (M012) was generated from a recombinant protein that included amino acid residues within the C-terminal region of human Girdin.

Species Reactivity

Human

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:250
IHC: 1:50
ICC: 1:50
IP: 1:50

Conjugate

Unconjugated

Specificity

Clone M012 mouse monoclonal antibody detects a 250 kDa* protein on SDS-PAGE immunoblots of human A431 cells and human brain tissue. The antibody also works for immmunoprecipitation, immunohistochemistry, and immunocytochemistry.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

APE, Galpha, vesicle, GIV, Girders actin filament, HkRP1, GRDN, CCDC88A

UniProt Details

UniProt (Human): Q3V6T2
UniProt (Immunogen Species): Q3V6T2

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Anti-Girdin (C-terminus) Antibody (M012)

Anti-Girdin (C-terminus) Antibody
SKU: GM0121

Bulk Order Anti-Girdin (C-terminus) Antibody

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Anti-Girdin (C-terminus) Antibody (M012)

Anti-Girdin (C-terminus) Antibody SKU: GM0121

Bulk Order Anti-Girdin (C-terminus) Antibody

Product Details

Anti-Girdin (C-terminus) Antibody
SKU: GM0121