Our FXYD5/Dysadherin (Extracellular region) mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects human FXYD5/Dysadherin (Extracellular region) and is Protein G purified. It is great for use in WB, ICC, IP.
Immunocytochemical labeling of FXYD5 in paraformaldehyde fixed human MDA-MB-231 cells. The cells were labeled with mouse monoclonal FXYD5 (FM0311). The antibody was detected using goat anti-mouse Ig DyLight® 594.
The Na+/K+ ATPase is an integral membrane heterodimer belonging to the P-type ATPase family. This ion channel uses the energy derived from ATP hydrolysis to maintain membrane potential by driving Na+ export and K+ import across the plasma membrane. It is composed of a large catalytic α subunit and a membrane-spanning auxiliary β subunit. In humans, the Na+/K+ ATPase is a binary complex of an α subunit that has four isoforms (α1-α4) and a β-subunit that has three isoforms (β1, β2, β3). In addition, the Na+/K+ ATPase can interact with a group of regulatory subunits, the FXYD type I membrane protein family. This family contains FXYD1-7, and each member contains the conserved F-X-Y-D motif in the trans-membrane domain. FXYD5 (Dysadherin) is expressed in a variety of cells and tissue, as well as upregulated in carcinomas. FXYD5 contains extensive O-glycosylation, and is expressed as molecules that range from 35 to 55 kDa depending on cell type. FXYD5 can reduce E-cadherin mediated cell-cell adhesions, and may be involved in carcinogenesis.
Protein G Purified
Monoclonal
M031
IgG1
ICC, IP, WB
Mouse
FXYD5
35-55
Clone (M031) was generated from a proprietary antigen related to the native human FXYD5 protein expressed in MDA-MB-231 breast cancer cell line.
Human
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
WB: 1:500
ICC: 1:100
IP: 1:50
Unconjugated
Clone M031 mouse monoclonal antibody detects a 35-55 kDa* band on SDS-PAGE immunoblots of human A431, A549, MeWo, MDA-MB-231 cell, as well as human breast, lung, and skin tissues. The antibody works for western blot, immunoprecipitation, ELISA, and immunocytochemistry, as well as detects the FXYD5 protein on live cells.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
United States
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
FXYD domain-containing ion transport regulator 5, Dysadherin, FXYD5, DYSAD, IWU1, HSPC113, UNQ2561/PRO6241
