Anti-Fascin (Ser-39), Phosphospecific Antibody (M315)

Name: Anti-Fascin (Ser-39), Phosphospecific Antibody
Brand: PhosphoSolutions
SKU: FM3151
Price: 309.0 USD
Availability: InStock

from PhosphoSolutions

Product Type

Primary Antibody

Species Reactivity

Chicken, Human, Mouse, Rat

Clone

M315

Applications

IHC, WB

Host Species

Mouse

Format

Antigen Affinity Purified

Gene Name

FSCN1

Molecular Weight

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Western blot analysis of mouse C2C12 cells treated with Calyculin A (100 nM) for 30 min (lanes 1 & 3) before treatment with lambda phosphatase (lanes 2 & 4). The blots were probed with anti-Fascin (clone 55K2) (lanes 1 & 2) and anti-Fascin (Ser-39) mouse monoclonal (lanes 3 & 4).

Volume: 100 µL

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Product Details

Target

Fascin (Ser-39)

Target Description

Fascin is an actin filament bundling protein localized to lamellipodia and filopodia where it has important roles in cell motility. Regulation of fascin occurs through PKC-mediated phosphorylation of Ser-39 in the F-actin binding site. Cell permeant peptides that block PKC phosphorylation of Ser-39 increase cell migration, while peptides that block fascin binding to F-actin alter lamellipodial morphology and cause aberrant cell motility. Studies using RNA interference of fascin show that fibroblasts have reduced number and abnormal morphology of filopodia, while Ser-39 phosphorylation status may determine filopodial frequency. In Drosophila neurons, fascin deficiency causes alterations in actin filaments and leads to abnormal morphology of developing neurons. Thus, fascin is a critical element of actin-based motility in various cell types.

Format

Antigen Affinity Purified

Clonality

Monoclonal

Clone

M315

Isotype

IgG1

Applications

IHC, WB

Host Species

Mouse

Gene Name

FSCN1

Molecular Weight

Antigen

Clone M315 was generated from a phospho-Fascin (Ser-39) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding serine 39 in human fascin. This sequence has high homology to the conserved site in rat and mouse fascin, as well as to the conserved region in fascin-2, but has low homology to the conserved region in fascin-3.

Species Reactivity

Chicken, Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:500
IHC: 1:100

Conjugate

Unconjugated

Specificity

The antibody detects a 55 kDa* band corresponding to fascin on SDS-PAGE immunoblots of mouse C2C12 cells treated with Calyculin A. This band is not observed after lambda phosphatase treatment.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser-39

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

p55

UniProt Details

UniProt (Human): Q16658
UniProt (Immunogen Species): Q16658

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Anti-Fascin (Ser-39), Phosphospecific Antibody (M315)

Anti-Fascin (Ser-39), Phosphospecific Antibody
SKU: FM3151

Bulk Order Anti-Fascin (Ser-39), Phosphospecific Antibody

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Anti-Fascin (Ser-39), Phosphospecific Antibody (M315)

Anti-Fascin (Ser-39), Phosphospecific Antibody SKU: FM3151

Bulk Order Anti-Fascin (Ser-39), Phosphospecific Antibody

Product Details

Anti-Fascin (Ser-39), Phosphospecific Antibody
SKU: FM3151