Anti-Crk II (Tyr-251), Phosphospecific Antibody

Name: Anti-Crk II (Tyr-251), Phosphospecific Antibody
Brand: PhosphoSolutions
SKU: CP3091
Price: 329.0 USD
Availability: InStock

1 Citation

Our Crk II (Tyr-251) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, rat, and Xenopus Crk II (Tyr-251) and is antigen affinity purified. It is great for use in WB, IHC.

Western blot of human K562 cells stimulated with pervanadate (1 mM) for 30 min. (lanes 1-6). The blot was treated with akaline phosphatase to dephosphorylate Crk (lanes 2, 4 & 6), then the blot was probed with rabbit polyclonals CrkL (C-terminus) CP3081 (lanes 1 & 2), Crk (Tyr-251) phospho-specific CP3091 (lanes 3 & 4), and mouse monoclonal anti-Crk II (C-terminal region) (lanes 5 & 6).

Species Reactivity

Chicken, Frog, Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Rabbit

Go to Full Product Details

from PhosphoSolutions

SKU: CP3091

Volume: 100 µL

Variant

Price:

$329

Quantity:

Product Details

Target

Crk II (Tyr-251)

Target Description

The Crk family of adaptor proteins (Crk I, Crk II and CrkL) are Src Homology 2 (SH2) and Src Homology 3 (SH3) domain-containing proteins that form protein complexes important for transmiting signals downstream of tyrosine kinases. Both Crk II and CrkL are composed of a single SH2 domain, followed by two tandem SH3 domains. Crk II is also alternatively spliced to a minor product, Crk I, which is structurally and functionally more similar to the v-Crk oncogene. Both Crk II and CrkL are ubiquitously expressed and their SH domains are highly homologous, however both are required for mouse development and have distinct non-overlapping phenotypes in knockout mice. Phosphorylation may be important for regulating Crk activity. Crk II Tyr-221 (CrkL Tyr-207) phosphorylation is a negative regulatory site, while Crk Tyr-251 phosphorylation in the SH3 domain is a positive regulatory site. EGF stimulation induces phosphorylation of Tyr-251, which increases binding of Crk to the SH2 domain of Abl, and promotes transactivation of Abl.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, IHC, WB

Host Species

Rabbit

Gene Name

CRK

Molecular Weight

Antigen

Phospho-Crk (Tyr-251) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding Tyr-251 in human Crk II. This peptide sequence is well conserved in mouse and rat Crk II, as well as in CrkL (Tyr-251). The site is not found in Crk I.

Species Reactivity

Chicken, Frog, Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:100

Conjugate

Unconjugated

Specificity

The antibody detects a 38 and 40 kDa* proteins corresponding to the molecular mass of Crk II and CrkL on SDS-PAGE immunoblots of human K562 cells stimulated with pervanadate and A431 cells stimulated with EGF. This reactivity is not observed after akaline phosphatase treatment.

Post Translational Modification Type

Phosphorylated

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

v-crk sarcoma virus CT10 oncogene homolog, CRKII, CRKL

UniProt Details

UniProt (Human): P46108
UniProt (Immunogen Species): P46108

Product Specific References for Applications and Species

Immunohistochemistry: Human

Immunohistochemistry: Human
PMID	Dilution	Publication
26473374	1:100	Kumar, S, et al. 2015. Reciprocal regulation of Abl kinase by Crk Y251 and Abi1 controls invasive phenotypes in glioblastoma. Oncotarget, 37792-37807.

Recover password

Create my account

Anti-Crk II (Tyr-251), Phosphospecific Antibody

Our Crk II (Tyr-251) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, rat, and Xenopus Crk II (Tyr-251) and is antigen affinity purified. It is great for use in WB, IHC.

SKU: CP3091

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-Crk II (Tyr-251), Phosphospecific Antibody

Related Products

Recently Viewed