Our Cdc42 mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Cdc42 and is antigen affinity purified. It is great for use in WB, ICC.
Immunocytochemical labeling in paraformaldehyde fixed and NP-40 permeabilized rat A7r5 cells. The cells were labeled with mouse monoclonal Anti-Cdc42 (CM4301) in the absence (Left) or presence of full length Cdc42 recombinant protein (Right), then the antibody was detected using Goat anti-Mouse secondary antibody conjugated to DyLight® 594.
Cdc42 proteins are Rho GTPase family members, which act as molecular switches in regulating a variety of biological response pathways including cell motility, cell-cycle progression, gene transcription, and cell transformation. Cdc42 GTPases regulate molecular events by cycling from the inactive GDP-bound state to active GTP-bound forms. The ratio of GDP versus GTP, which is bound to the GTPase, is determined by the opposing effects of guanine nucleotide exchange factors and GTPase activating proteins. Active (GTP-bound) Cdc42 binds to the p21-binding domain of p21-activated protein kinase 1 (PAK1) to regulate downstream signaling cascades.
Antigen Affinity Purified
Clone (M430) was generated from a rat recombinant Cdc42 protein. This sequence is highly conserved in human and mouse Cdc42.
Human, Mouse, Rat
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
The antibody detects a 21 kDa* protein in human Jurkat cells, rat A7r5 cells, and mouse brain. The antibody also detects recombinant full length rat Cdc42 fusion protein, but does not recognize a full length human RhoA GST fusion protein.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.