Anti-Cdc42 Antibody (M430)

Name: Anti-Cdc42 Antibody
Brand: PhosphoSolutions
SKU: CM4301
Price: 255.0 USD
Availability: InStock

from PhosphoSolutions

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Clone

M430

Applications

ICC, WB

Host Species

Mouse

Format

Antigen Affinity Purified

Gene Name

CDC42

Molecular Weight

Go to Full Product Details

Immunocytochemical labeling in paraformaldehyde fixed and NP-40 permeabilized rat A7r5 cells. The cells were labeled with mouse monoclonal Anti-Cdc42 (CM4301) in the absence (Left) or presence of full length Cdc42 recombinant protein (Right), then the antibody was detected using Goat anti-Mouse secondary antibody conjugated to DyLight® 594.

Volume: 100 µL

Variant

Price:

$255

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Product Details

Target

Cdc42

Target Description

Cdc42 proteins are Rho GTPase family members, which act as molecular switches in regulating a variety of biological response pathways including cell motility, cell-cycle progression, gene transcription, and cell transformation. Cdc42 GTPases regulate molecular events by cycling from the inactive GDP-bound state to active GTP-bound forms. The ratio of GDP versus GTP, which is bound to the GTPase, is determined by the opposing effects of guanine nucleotide exchange factors and GTPase activating proteins. Active (GTP-bound) Cdc42 binds to the p21-binding domain of p21-activated protein kinase 1 (PAK1) to regulate downstream signaling cascades.

Format

Antigen Affinity Purified

Clonality

Monoclonal

Clone

M430

Isotype

IgM

Applications

ICC, WB

Host Species

Mouse

Gene Name

CDC42

Molecular Weight

Antigen

Clone (M430) was generated from a rat recombinant Cdc42 protein. This sequence is highly conserved in human and mouse Cdc42.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:500
ICC: 1:200

Conjugate

Unconjugated

Specificity

The antibody detects a 21 kDa* protein in human Jurkat cells, rat A7r5 cells, and mouse brain. The antibody also detects recombinant full length rat Cdc42 fusion protein, but does not recognize a full length human RhoA GST fusion protein.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

CDC-42, G25K

UniProt Details

UniProt (Human): P60953
UniProt (Immunogen Species): Q8CFN2

Product Specific Protocols

Western Blot Protocol: Download

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Anti-Cdc42 Antibody (M430)

Anti-Cdc42 Antibody
SKU: CM4301

Bulk Order Anti-Cdc42 Antibody

Product Details

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