Immunocytochemical labeling of CD63 in paraformaldehyde fixed human MeWo melanoma cells. The cells were labeled with mouse monoclonal CD63 (CM0401). The antibody was detected using goat anti-mouse Ig DyLight® 594.
Anti-CD63 (Extracellular region) Antibody
SKU: CM0401
Bulk Order Anti-CD63 (Extracellular region) Antibody
Product Details
CD63 (Extracellular region)
Tetraspanins comprise a large superfamily of cell surface-associated membrane proteins with four transmembrane domains. On cell membranes, tetraspanins form networks of various proteins called tetraspanin-enriched microdomains (TEMs). CD63 was the first characterized tetraspanin and it is found in TEMs, as well as late endosomes and lysosomes. In late endosomes, CD63 is enriched on the intraluminal vesicles, and can be secreted as exosomes through fusion of endosomes with the plasma membrane. The complex localization pattern of CD63 suggests that its intracellular trafficking and distribution must be tightly regulated. CD63 contains N-link glycosylation sites that produce diverse CD63 molecules that range from 30 to 60 kDa depending on cell type. CD63 is an important exosomal marker in cancer cells, and may be involved in cancer progression and metastasis.
Protein G Purified
Monoclonal
M040
IgG2a
ICC, WB
Mouse
CD63
30-60
Clone (M040) was generated from a proprietary antigen related to the native human CD63 expressed in MeWo melanoma cell line.
Human
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
WB: 1:100
ICC: 1:500
Unconjugated
Clone M040 mouse monoclonal antibody has high reactivity to CD63 at the plasma membrane in immunocytochemical assays, and weakly detects a 30-60 kDa* protein on SDS-PAGE "Native" immunoblots of human A431, MeWo, and A549 cells. The antibody does not detect denatured CD63 in western blot assays.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
United States
After date of receipt, stable for at least 1 year at -20°C.
