Anti-Caspase-3 (N-terminal region) Antibody (M377)

Name: Anti-Caspase-3 (N-terminal region) Antibody
Brand: PhosphoSolutions
SKU: CM3771
Price: 275.0 USD
Availability: InStock

1 Citation

Our Caspase-3 (N-terminal region) primary antibody from PhosphoSolutions is mouse monoclonal. It detects human Caspase-3 (N-terminal region) and is Protein A purified. It is great for use in WB, ICC.

Inhibition of protein biosynthesis as shown by puromycin Western blot analysis and induction of apoptosis in LNCaP cells as demonstrated by PARP and Caspase-3 (cat. CM3771) cleavage after combination treatment. Image from publication CC-BY-4.0. PMID: 37484018

Western blot analysis of Caspase expression in human Jurkat cells. The blot was probed with anti-Caspase-3 at 1:500 (lane 1) and 1:1000 (lane 2), anti-Caspase-6 at 1:250 (lane 3) and 1:500 (lane 4), anti-Caspase-7 at 1:500 (lane 5) and 1:1000 (lane 6), as well as anti-Caspase-8 at 1:250 (lane 7) and 1:500 (lane 8).

Species Reactivity

Human

Applications

ICC, WB

Host Species

Mouse

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SKU: CM3771

Product Details

Target

Caspase-3 (N-terminal region)

Target Description

The caspases are a group of cysteine enzymes, which cleave proteins in response to intrinsic and extrinsic pathways that cause apoptotic cell death. The caspases can be grouped into two subgroups based on their roles in apoptosis. Initiator caspases (caspases 2, 8, 9, and 10) are activated through the apoptosis-signaling pathways and activate the effector caspases (caspases 3, 6, and 7) which carry out apoptosis. Caspase cascades are initiated through assembly of multiprotein complexes that trigger activation of the initiator caspases, which are then released and are able to activate the downstream effector caspases.

Format

Protein A Purified

Clonality

Monoclonal

Clone

M377

Isotype

IgG2a

Applications

ICC, WB

Host Species

Mouse

Gene Name

CASP3

Molecular Weight

Antigen

Clone (M377) was generated from a recombinant protein containing amino acid residues in the N-terminal region of human Caspase-3.

Species Reactivity

Human

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:100

Conjugate

Unconjugated

Specificity

The antibody detects a 32 kDa* protein corresponding to the molecular weight of Caspase-3 on SDS-PAGE immunoblots of human Jurkat and K562 cells.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Caspase,-3, CPP-32, Apoptain, Yama, SCA-1, CASP-2, NEDD2, ICH-1, p18, p13, p12

UniProt Details

UniProt (Human): P42574
UniProt (Immunogen Species): P42574

Product Specific References for Applications and Species

Western Blot: Human

Western Blot: Human
PMID	Dilution	Publication
37484018	not listed	Masilamani, AP, et al. 2023. Enhanced Cytotoxicity of a Pseudomonas Exotoxin A Based Immunotoxin Against Prostate Cancer by Addition of the Endosomal Escape Enhancer SO1861. Frontiers in Pharmacology, 1211824.

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Anti-Caspase-3 (N-terminal region) Antibody (M377)

SKU: CM3771

Product Details

Product Specific References for Applications and Species

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