Anti-CaM Kinase II (Thr286) Antibody
Our Anti-CaM Kinase II (Thr286) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, rabbit, and rat CaM Kinase II (Thr286) and is antigen affinity purified from pooled serum. It is great for use in WB, IHC.
Primary Antibody
Human, Mouse, Rabbit, Rat
Zebrafish
ICC, IHC, WB
Rabbit
Antigen Affinity Purified from Pooled Serum
CAMK2A/B
50/60 kDa
Western blot of rat brain lysate showing specific immunolabeling of the ~50 kDa α- and the ~60 kDa β-CaM Kinase II phosphorylated at Thr286 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).
Product Specific References for Applications and Species
| Immunocytochemistry: Human | ||
| PMID | Dilution | Publication |
| 26057350 | not listed | Samikkannu, T., et al. 2015. HIV subtypes B and C gp120 and methamphetamine interaction: dopaminergic system implicates differential neuronal toxicity. Scientific reports, 5, p.11130. |
| Immunocytochemistry: Rat | ||
| PMID | Dilution | Publication |
| 32492405 | not listed | Li, B., et al. 2020. Neuronal Inactivity Co-opts LTP Machinery to Drive Potassium Channel Splicing and Homeostatic Spike Widening. Cell, S0092-8674(20), p.p. 30575-4. |
| Immunohistochemistry: Rabbit | ||
| PMID | Dilution | Publication |
| 22593045 | 1:1000 | Kothmann, W.W., et al. 2012. Nonsynaptic NMDA Receptors Mediate Activity-Dependent Plasticity of Gap Junctional Coupling in the All Amacrine Cell Network. J. Neurosci. 32(3): 6747-6759. |
| Western Blot: Human | ||
| PMID | Dilution | Publication |
| 30143647 | 1:1000 | Lee, S.H., et al. 2018. Reciprocal control of excitatory synapse numbers by Wnt and Wnt inhibitor PRR7 secreted on exosomes. Nature communications, 9(1), p.3434. |
| 28318784 | not listed | Murakoshi, H., et al. 2017. Kinetics of Endogenous CaMKII Required for Synaptic Plasticity Revealed by Optogenetic Kinase Inhibitor. Neuron. Apr 5;94(1):37-47.e5. |
| 26057350 | not listed | Samikkannu, T., et al. 2015. HIV subtypes B and C gp120 and methamphetamine interaction: dopaminergic system implicates differential neuronal toxicity. Scientific reports, 5, p.11130. |
| 24855644 | 1:3000 | Coultrap, S.J., et al. 2014. Nitric oxide induces Ca2+-independent activity of the Ca2+/calmodulin-dependent protein kinase II (CaMKII). J. Biol Chem. 289(28):19458-65. |
| 23255606 | 1:10,000 | Magupalli, V.G., et al. 2013. Ca2+-independent activation of Ca2+/calmodulin-dependent protein kinase II bound to the C-terminal domain of CaV2. 1 calcium channels. Journal of Biological Chemistry, 288(7), 4637-4648. |
| Western Blot: Mouse | ||
| PMID | Dilution | Publication |
| 34965414 | 1:2000 | Buonarati, O.R., et al. 2021. Conserved and Divergent Features of Neuronal CaMKII Holoenzyme Structure, Function, and High-Order Assembly. Cell Reports, 110168. |
| 34704002 | 1:2500 | Tullis, J.E., et al. 2021. GluN2B S1303 Phosphorylation by CaMKII or DAPK1: No Indication for Involvement in Ischemia or LTP. iScience, 103214. |
| 33881939 | 1:2000 | Goodell, D.J., et al. 2021. Young DAPK1 knockout mice have altered presynaptic function. Journal of Neurophysiology, 125(5), pp1973-1981. |
| 33853773 | 1:1000 | Cook, S.G., et al. 2021. CaMKII holoenzyme mechanisms that govern the LTP versus LTD decision. Science Advances, 7(16), p.eabe2300. |
| 33449853 | 1:1000 | Kreitmeier, K.G., et al. 2021. CaMKIIδ Met281/282 oxidation is not required for recovery of calcium transients during acidosis. American Journal of Physiology-Heart and Circulatory Physiology, January 15. |
| 31914378 | 1:2500 | Buonarati, O.R., et al. 2020. CaMKII versus DAPK1 Binding to GluN2B in Ischemic Neuronal Cell Death after Resuscitation from Cardiac Arrest. Cell Reports, 30(1), pp.1-8. |
| 30282026 | 1:1000 | Glasgow, S.D., et al. 2018. Activity-dependent netrin-1 secretion drives synaptic insertion of GluA1-containing AMPA receptors in the hippocampus. Cell Rep, 25(1):168-182. |
| 27622724 | not listed | Huang, L., et al. 2017. Long-lasting behavioral effects in neonatal mice with multiple exposures to ketamine-xylazine anesthesia. Neurotoxicology and Teratology. |
| 18417701 | 1:10,000 | Sun, X., et al. 2008. Acute and Chronic Dopamine Receptor Stimulation Modulates AMPA Receptor Trafficking in Nucleus Accumbens Neurons Cocultured with Prefrontal Cortex Neurons. J. Neurosci., 28: 4216 - 4230. |
| 17855343 | not lited | Townsend, M., et al. 2008. Soluble A-beta Inhibits Specific Signal Transduction Cascades Common to the Insulin Receptor Pathway. J. Biol. Chem., 282: 33305 - 33312. |
| Western Blot: Rat | ||
| PMID | Dilution | Publication |
| 36331254 | not listed | Kazami, M., et al. 2022. Ca2+/Calmodulin induces translocation of membrane-associated TSC2 to the nucleus where it suppresses CYP24A1 expression. Bioscience, Biotechnology, and Biochemistry, zbac174. |
| 35620430 | 1:2500 | Brown, C.N., et al. 2022. Aβ-induced Synaptic Impairments Require CaMKII Activity That is Stimulated by Indirect Signaling Events. iScience, 104368. |
| 34687697 | 1:1000 | Rumian, N.L., et al. 2021. CaMKIIα knockout protects from ischemic neuronal cell death after resuscitation from cardiac arrest. Brain Research, 1773, p147699. |
| 34667946 | not listed | Brown, C.N., et al. 2021. Characterization of Six CaMKIIα Variants Found in Patients with Schizophrenia. iScience, 103184. |
| 34544759 | 1:1000 | Murray, C.H., et al. 2021. CaMKII Modulates Diacylglycerol Lipase-α Activity in the Rat Nucleus Accumbens after Incubation of Cocaine Craving. eNeuro, 8(5). |
| 33444640 | 1:1000 | Murray, C.H., et al. 2021. mGlu5 function in the nucleus accumbens core during the incubation of methamphetamine craving. Neuropharmacology, p.108452. |
| 30143647 | 1:1000 | Lee, S.H., et al. 2018. Reciprocal control of excitatory synapse numbers by Wnt and Wnt inhibitor PRR7 secreted on exosomes. Nature communications, 9(1), p.3434. |
| 28318784 | not listed | Murakoshi, H., et al. 2017. Kinetics of Endogenous CaMKII Required for Synaptic Plasticity Revealed by Optogenetic Kinase Inhibitor. Neuron. Apr 5;94(1):37-47.e5. |
| 24843070 | 1:3000 | Barcomb, K., et al. 2014. Autonomous CaMKII requires further stimulation by Ca2+/calmodulin for enhancing synaptic strength. The FASEB Journal, 28(8), 3810-3819. |
| 24780380 | 1:2000 | Bernard, P.B., et al. 2014. Necessary, but not sufficient: insights into the mechanisms of mGluR mediated long-term depression from a rat model of early life seizures. Neuropharmacology, 84, 1-12. |
| 23143515 | 1:1000 | Shin, S.M., et al. 2012. GKAP orchestrates activity-dependent postsynaptic protein remodeling and homeostatic scaling. Nature neuroscience, 15(12), p.1655-1666. |
| 20424167 | not listed | Vest, R.S., et al. 2010. Dual Mechanism of a Natural CaMKII Inhibitor. Mol. Biol. Cell, 18: 5024-5033. |
| 20353941 | not listed | Coultrap, S.J., et al. 2010. CaMKII Autonomy Is Substrate-dependent and Further Stimulated by Ca2+/Calmodulin. J. Biol. Chem., 285: 17930 - 17937. |
| 18819923 | 1:1000 | Davies, K.D., et al. 2008. Long Term Synaptic Depression That Is Associated with GluR1 Dephosphorylation but Not Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid (AMPA) Receptor Internalization. J. Biol. Chem., 283: 33138 - 33146. |
| 18417701 | 1:10,000 | Sun, X., et al. 2008. Acute and Chronic Dopamine Receptor Stimulation Modulates AMPA Receptor Trafficking in Nucleus Accumbens Neurons Cocultured with Prefrontal Cortex Neurons. J. Neurosci., 28: 4216 - 4230. |
| 17942605 | 1:500 | Sun, X., et al. 2008. Dual Mechanism of a Natural CaMKII Inhibitor. Mol. Biol. Cell, 18: 5024 - 5033. |
Product Specific Protocols
- Western Blot Protocol: Download