Name: Anti-Calbindin Antibody
Brand: PhosphoSolutions
SKU: 302-CALB
Price: 415.0 USD
Availability: InStock

Our Anti-Calbindin primary antibody from PhosphoSolutions is mouse monoclonal. It detects bovine, human, mouse, and rat Calbindin and is Protein G purified. It is great for use in WB, IHC.

Product Type

Primary Antibody

Species Reactivity

Bovine, Human, Mouse, Rat

Applications

IHC, WB

Host Species

Mouse

Format

Protein G Purified

Gene Name

CALB1

Molecular Weight

28 kDa

Go to Full Product Details

Immunofluorescence of a section of rat cerebellum showing specific labeling of calbindin (cat. 302-CALB, 1:1000, red) in the dendrites of Purkinje cells. Axons are labeled green with anti-Neurofilament H antibody ( cat. 1451-NFH , 1:25,000), and additional nuclear staining with DAPI.

Immunofluorescence of a section of rat cerebellum labeled with anti-calbindin (cat. 302-CALB, 1:2,000, red), colabeled with anti-GFAP ( cat. 620-GFAP , 1:5000,green), and DAPI staining of nuclear DNA. The anti-calbindin prominently labels the dendrites and perikarya of Purkinje cells in the molecular layer of the cerebellum. The anti-GFAP labels the processes of Bergmann glia in the molecular layer and the astroglia in the granular and white layers of the cerebellum.

Western blot of rat cerebellar lysate showing specific immunolabeling of the ~ 28 kDa calbindin protein.

Volume: 100 µL

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Product Details

Target

Calbindin

Target Description

Calbindin (aka. calbindin D28k) is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. It is predominantly expressed in certain types of neurons, particularly in dendrites and perikarya of the cerebellum and is thought to buffer entry of calcium upon stimulation of glutamate receptors (Andressen et al., 1993). Calbindin has recently been shown to play a critical role in mitochondrial dysfunction and loss of synaptic proteins in vivo in an Alzheimer’s disease mouse model (Kook et al., 2014).

Format

Protein G Purified

Clonality

Monoclonal

Isotype

IgG2a

Applications

IHC, WB

Host Species

Mouse

Gene Name

CALB1

Molecular Weight

28 kDa

Antigen

Recombinant full length human calbindin purified from E. coli.

Species Reactivity

Bovine, Human, Mouse, Rat

Antibody Registry ID

AB_2560939

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Protein G purified.

Buffer

PBS + 50% glycerol and 5 mM NaN3

Dilution Ranges

WB: 1:2000
WB Brain: 1:2000
IHC: 1:1000-1:5000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~28 kDa calbindin protein.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

avian-type antibody, CAB27 antibody, CALB 1 antibody, CALB antibody, CALB1 antibody, CALB1_HUMAN antibody, Calbindin 1 28kDa antibody, Calbindin antibody, Calbindin D28 antibody, D 28K antibody, D-28K antibody, D28K antibody, OTTHUMP00000166027 antibody, OTTHUMP00000225441 antibody, RTVL H protein antibody, Vitamin D dependent calcium binding protein antibody, Vitamin D dependent calcium binding protein avian type antibody, Vitamin D-dependent calcium-binding protein antibody

UniProt Details

UniProt (Human): P05937
UniProt (Immunogen Species): P05937

NCBI Gene ID

793

Product Specific Protocols

Western Blot Protocol: Download

General References

Andressen, C., Blümcke, I., & Celio, M. R. (1993). Calcium-binding proteins: selective markers of nerve cells. Cell and Tissue Research, 271(2), 181-208. PMID: 8453652
Kook, S.Y., Jeong, H., Kang, M.J., Park, R., Shin, H.J., Han, S.H., Son, S.M., Song, H., Baik, S.H., Moon, M. and Yi, E.C., 2014. Crucial role of calbindin-D 28k in the pathogenesis of Alzheimer’s disease mouse model. Cell Death and Differentiation, 21(10), p.1575. PMID: 24853300

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Anti-Calbindin Antibody

Our Anti-Calbindin primary antibody from PhosphoSolutions is mouse monoclonal. It detects bovine, human, mouse, and rat Calbindin and is Protein G purified. It is great for use in WB, IHC.