Anti-β-Dystroglycan (Tyr-892), Phosphospecific Antibody (M117)

Name: Anti-β-Dystroglycan (Tyr-892), Phosphospecific Antibody
Brand: PhosphoSolutions
SKU: DM1171
Price: 309.0 USD
Availability: InStock

from PhosphoSolutions Datasheet

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Clone

M117

Applications

Host Species

Mouse

Format

Protein A Purified

Gene Name

DAG1

Molecular Weight

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Western blot analysis of HepG2 cells untreated (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 min (lanes 2 & 4). Blots were probed with anti-β-Dystroglycan (lanes 1 & 2) and anti-β-Dystroglycan (Tyr-892) (lanes 3 & 4).

Volume: 100 µL

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Product Details

Target

β-Dystroglycan (Tyr-892)

Target Description

Dystroglycans are essential elements of the neuromuscular junction (NMJ). The gene for dystroglycan is expressed as a precursor protein that is post-translationally cleaved into a 156 kDa extracellular peripheral membrane protein called α-dystroglycan and a 43 kDa transmembrane protein, β-Dystroglycan. The latter protein contains a PPxY motif that promotes binding to WW domain-containing proteins, such as utrophin and dystrophin. Phosphorylation at tyrosine 892 within the PPxY motif may regulate c-Src interactions with β-Dystroglycan, as well as inhibit interactions with WW domain proteins. In skeletal muscle, β-Dystroglycan is normally localized to the plasma membrane, however phosphorylation of Tyr-892 leads to localization of β-Dystroglycan to endosomal compartments along with c-Src. Thus, phosphorylation at Tyr-892 may have important roles in altering the localization of β-Dystroglycan during NMJ formation.

Format

Protein A Purified

Clonality

Monoclonal

Clone

M117

Isotype

IgG1

Applications

Host Species

Mouse

Gene Name

DAG1

Molecular Weight

Antigen

Clone (M117) was generated from a synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 892 of human dystroglycan. This peptide sequence has high homology to the conserved tyrosine site in rat and mouse dystroglycan.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

The antibody detects a 43 kDa* protein on SDS-PAGE immunoblots of human HepG2 or HeLa cells treated with pervanadate, but not in control cells.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Tyr-892

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

UniProt Details

UniProt (Human): Q14118
UniProt (Immunogen Species): Q14118

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Anti-β-Dystroglycan (Tyr-892), Phosphospecific Antibody (M117)

Anti-β-Dystroglycan (Tyr-892), Phosphospecific Antibody
SKU: DM1171

Bulk Order Anti-β-Dystroglycan (Tyr-892), Phosphospecific Antibody

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Anti-β-Dystroglycan (Tyr-892), Phosphospecific Antibody (M117)

Anti-β-Dystroglycan (Tyr-892), Phosphospecific Antibody SKU: DM1171

Bulk Order Anti-β-Dystroglycan (Tyr-892), Phosphospecific Antibody

Product Details

Anti-β-Dystroglycan (Tyr-892), Phosphospecific Antibody
SKU: DM1171